FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655564
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23390.3567920142045628No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22350.34092781177733983No Hit
GACTTACACTACCCCTCGCACTTTTGGCCAGGGGACCAAGTTGGAGATTAAACGAACTGTGGCTGCACCAT17470.26648809269575513No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17250.2631322037207656No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15120.23064109682654935No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15030.22926823315496275No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14920.227590288667468No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14700.22423439969247852No Hit
GTGTAAGTCTGTTGACAGTGGTAGGTTGCAAAATCTTCAGGTTGCAGAGTGTTGATGGTGAGAGTGAAATCTGTC14620.223014076428846No Hit
GTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT13970.21309894991183165No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13960.21294640950387755No Hit
ACACTACCCCTCGCACTTTTGGCCAGGGGACCAAGTTGGAGATTAAACGA13910.21218370746410725No Hit
CCATCAACACTCTGCAACCTGAAGATTTTGCAACCTACCACTGTCAACAG13060.19921777278801156No Hit
GGGTAGTGTAAGTCTGTTGACAGTGGTAGGTTGCAAAATCTTCAGGTTGCAGAGTGTTGATGGTGAGAGTGAAAT12740.1943364797334814No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12550.19143821198235414No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12350.18838740382327276No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12030.18350611076874265No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11930.18198070668920197No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11560.1763367115949015No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11220.1711503377244632No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11200.17084525690855507No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11150.17008255486878474No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10880.16596396385402495No Hit
CTATTTAAGTTGGTATCAGCAGAAACCAGGGAAAGCCCCTGAACTCCTCATCTACGCAGTCTCCACTTTACAAA10750.16398093855062207No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10600.16169283243131105No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9760.14887943816316943No Hit
ACCTACCACTGTCAACAGACTTACACTACCCCTCGCACTTTTGGCCAGGGGACCAAGTTGGAGATTAAACGAA9730.14842181693930723No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC9560.14582863000408808No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9460.14430322592454742No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9090.13865923083024695No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAACTTGGTCC8830.1346931802234412No Hit
GTTCAGGGGCTTTCCCTGGTTTCTGCTGATACCAACTTAAATAGTTGCCA8540.13026950839277324No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8510.12981188716891104No Hit
CCCCTGAACTCCTCATCTACGCAGTCTCCACTTTACAAACTGGAGTCCCA8350.12737124064164598No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8200.12508313452233497No Hit
GTCTGCATCTGTGGGAGACAGAGTCAGCATCACTTGTCGGGCAAGTCAGA8200.12508313452233497No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8190.1249305941143809No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8040.1226424879950699No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7970.12157470513939142No Hit
CTTTTGGCCAGGGGACCAAGTTGGAGATTAAACGAACTGTGGCTGCACCA7960.12142216473143737No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAACTTGGTCCCCTGGCCAAAAGTG7950.1212696243234833No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7910.12065946269166701No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7770.11852389698031009No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7770.11852389698031009No Hit
CATCTACGCAGTCTCCACTTTACAAACTGGAGTCCCACCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA7690.11730357371667756No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7610.116083250453045No Hit
GTGGTAGGTTGCAAAATCTTCAGGTTGCAGAGTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA7560.11532054841327467No Hit
GATTTCACTCTCACCATCAACACTCTGCAACCTGAAGATTTTGCAACCTACCACTGTCAACAGACTTACACTACC7520.11471038678145841No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT7370.1124222806621474No Hit
AGTCAGACCATTGGCAACTATTTAAGTTGGTATCAGCAGAAACCAGGGAA7100.1083036896473876No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7090.10815114923943352No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7080.10799860883147945No Hit
GTCTCCACTTTACAAACTGGAGTCCCACCAAGGTTCAGTGGCAGTGGATC7040.10738844719966319No Hit
GGCCAAAAGTGCGAGGGGTAGTGTAAGTCTGTTGACAGTGGTAGGTTGCA6980.10647320475193878No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC6790.10357493700081152No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6780.10342239659285744No Hit
GTCTCCGTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCAGCATCACTTGTCGGGCAAGTCAGACCATTGGCAA6760.10311731577694933No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6730.10265969455308711No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6670.1017444521053627No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6620.10098175006559237No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG6570.10021904802582204No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6560.10006650761786796No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGATT200.00679441552.80258651
TCGACGA200.007048730352.31424746
CGCGGGA350.001297729639.73080444
GTATCAA23400.038.7409821
GCAATGT650.001504870838.54686470
CGTCGGA851.2624635E-436.84626870
ATAGCGG857.678682E-834.1857363
TATACTG400.002809281733.9475865
CGGCATA450.003312301132.8250866
TATCAAC28450.031.9787482
TACTCCG851.3907993E-731.9506725
ATCAACG28850.031.8883743
TCAACGC28950.031.7782294
ACGAACC901.6414924E-731.35242752
ACGAGAC1950.031.345822
CAACGCA29350.031.3451375
AACGCAG29500.031.3008276
CAAACGA901.7112689E-731.20402349
AATCGTT902.0199877E-730.61946139
ATCTCCG2000.030.5481710
Produced by FastQC (version 0.11.5)