FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004687940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655564
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22200.3386397056580288No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19210.2930301236797628No Hit
GACTTACACTACCCCTCGCACTTTTGGCCAGGGGACCAAGTTGGAGATTAAACGAACTGTGGCTGCACCAT18750.2860132649138757No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18530.28265737593888624No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17550.26770841595938766No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15100.23033601601064121No Hit
GTGTAAGTCTGTTGACAGTGGTAGGTTGCAAAATCTTCAGGTTGCAGAGTGTTGATGGTGAGAGTGAAATCTGTC14320.21843786419022398No Hit
GTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT14300.21813278337431585No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13680.2086752780811637No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13470.2054719295141283No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13450.20516684869822016No Hit
GGGTAGTGTAAGTCTGTTGACAGTGGTAGGTTGCAAAATCTTCAGGTTGCAGAGTGTTGATGGTGAGAGTGAAAT13340.2034889042107254No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12620.1925059948380326No Hit
CCATCAACACTCTGCAACCTGAAGATTTTGCAACCTACCACTGTCAACAG12250.1868619997437321No Hit
ACACTACCCCTCGCACTTTTGGCCAGGGGACCAAGTTGGAGATTAAACGA11960.18243832791306416No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11920.1818281662812479No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11810.18015022179375317No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11670.17801465608239622No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11310.17252320139604982No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11220.1711503377244632No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10790.16459110018243833No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10500.1601674283517704No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10490.16001488794381633No Hit
CTATTTAAGTTGGTATCAGCAGAAACCAGGGAAAGCCCCTGAACTCCTCATCTACGCAGTCTCCACTTTACAAA10450.15940472631200006No Hit
ACCTACCACTGTCAACAGACTTACACTACCCCTCGCACTTTTGGCCAGGGGACCAAGTTGGAGATTAAACGAA10270.15665899896882685No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9760.14887943816316943No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9660.14735403408362877No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9050.13804906919843069No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8880.13545588226321154No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC8850.1349982610393493No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8790.13408301859162494No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG8790.13408301859162494No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAACTTGGTCCCCTGGCCAAAAGTG8730.13316777614390052No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8700.13271015492003832No Hit
GTGGTAGGTTGCAAAATCTTCAGGTTGCAGAGTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA8500.12965934676095697No Hit
GTCTGCATCTGTGGGAGACAGAGTCAGCATCACTTGTCGGGCAAGTCAGA8290.12645599819392156No Hit
GTTCAGGGGCTTTCCCTGGTTTCTGCTGATACCAACTTAAATAGTTGCCA8040.1226424879950699No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAACTTGGTCC8020.12233740717916175No Hit
CCCCTGAACTCCTCATCTACGCAGTCTCCACTTTACAAACTGGAGTCCCA8000.12203232636325363No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7940.12111708391552922No Hit
CTTTTGGCCAGGGGACCAAGTTGGAGATTAAACGAACTGTGGCTGCACCA7760.118371356572356No Hit
GTGGGAGACAGAGTCAGCATCACTTGTCGGGCAAGTCAGACCATTGGCAACTATTTAAGTTGGTATCAGCAGAAA7760.118371356572356No Hit
GATTTCACTCTCACCATCAACACTCTGCAACCTGAAGATTTTGCAACCTACCACTGTCAACAGACTTACACTACC7700.11745611412463162No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT7540.11501546759736656No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7500.11440530596555026No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7350.11211719984623926No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7300.11135449780646893No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7110.10845623005534166No Hit
GGCCAAAAGTGCGAGGGGTAGTGTAAGTCTGTTGACAGTGGTAGGTTGCA6960.10616812393603066No Hit
AGTCAGACCATTGGCAACTATTTAAGTTGGTATCAGCAGAAACCAGGGAA6840.10433763904058185No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6770.10326985618490338No Hit
CATCTACGCAGTCTCCACTTTACAAACTGGAGTCCCACCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA6760.10311731577694933No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6760.10311731577694933No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6680.10189699251331677No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC6670.1017444521053627No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT6590.10052412884173018No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6570.10021904802582204No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6560.10006650761786796No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)