FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269852
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACA60550.4768272208099842No Hit
GTCTATTACTGTGCGAGGAGCGAGGGTGAATATAGTGGCTATAGTGATTA44950.3539782588837124No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39070.30767365015765613No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33930.26719649218964103No Hit
CCATTAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTTCTGTCAACAG31120.24506792917599846No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29610.2331767796562119No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG27210.21427693935986242No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25240.19876332044994222No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC24330.19159713100424303No Hit
GATCTATGTTGCATCCAATCTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAGATCTGGGACAGAATTCA23930.1884471576215181No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23480.18490343756595257No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22830.17978473081902457No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGCCATTAGCAACTTTTTAAATTGGTATCAGCAAAAA22800.1795484828153202No Hit
GTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC22460.17687100544000403No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22300.17561101608691407No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21710.1709648053473948No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21610.1701773120017136No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21360.16820857863751051No Hit
GAATTCACTCTCACCATTAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTTCTGTCAACAGAGTTTCAGTAGC21340.16805107996837426No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20400.1606486425189707No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20310.15993989850785761No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20110.15836491181649515No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19890.15663242645599645No Hit
CTGCTAATGGTGAGAGTGAATTCTGTCCCAGATCTACTGCCACTGAACCT19890.15663242645599645No Hit
ACATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATT19870.1564749277868602No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19440.1530887064004309No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19430.1530099570658628No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG19300.1519862157164772No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19150.15080497569795534No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18960.149308738341161No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG18780.1478912503189348No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGCCATTAGCAA18070.14230004756459808No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17830.14041006353496313No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17690.13930757285100942No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17290.13615759946828448No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16890.13300762608555958No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16760.13198388473617398No Hit
CTCCTGTGCAGCCTCTGGGTTCACCTTCAGTAGATACACCATGAAGTGGGTCCGCCAGGCTCCAGGGCAGGGG16730.13174763673246961No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG16600.13072389538308402No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16340.12867641268431282No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACGTCCAGATGACCCAGTC16280.1282039166769041No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16140.12710142599295038No Hit
GTAGATACACCATGAAGTGGGTCCGCCAGGCTCCAGGGCAGGGGCTGGAG16040.12631393264726912No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15940.1255264393015879No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15850.12481769529047479No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCGGAGGGCTA15840.12473894595590668No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15690.12355770593738484No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15690.12355770593738484No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15480.12190396991145425No Hit
ATGTTGCATCCAATCTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAGATCTGGGACAGAATTCACTCTCA15470.12182522057688613No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15290.12040773255465992No Hit
CTCCAGAGACGACGCCAAGAACTCACTGTTTCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTCTATTA15210.11977773787811494No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15210.11977773787811494No Hit
GAGTGAATTCTGTCCCAGATCTACTGCCACTGAACCTTGATGGGACCCCA14560.11465903113118693No Hit
CACCATTAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTTCTGTCAACAGAGTTTCAGTAGCCCTCCGACGTT14490.11410778578921008No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14490.11410778578921008No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC14450.11379278845093758No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14390.11332029244352886No Hit
ATATTCACCCTCGCTCCTCGCACAGTAATAGACAGCCGTGTCCTCGGCTC14300.11261154843241573No Hit
CAGTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAA14050.11064281506821268No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13990.11017031906080395No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13920.10961907371882709No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC13860.10914657771141834No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACGT13830.10891032970771397No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13800.1086740817040096No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13680.10772908968919212No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC13500.10631160166696592No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13060.1028466309459685No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12850.10119289492003791No Hit
CTCCAGCCCCTGCCCTGGAGCCTGGCGGACCCACTTCATGGTGTATCTAC12770.10056290024349294No Hit
TACATATTCTACGCAGACTCACTGAAGGGCCGATTCACCATCTCCAGAGACGACGCCAAGAACTCACTGTTTCTG12730.10024790290522045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG17700.047.82220570
CGTATGC751.6370905E-1146.03293241
GTGGTAT2150.041.1671371
ATCTCGT955.456968E-1239.77422737
GTATCAA20050.036.3341641
CGTTTAG400.002075513136.11111563
GTCACCG18400.035.97217670
TATACAC1051.8189894E-1135.663113
TCGTCTC15300.034.73758365
CTCTGGA17800.034.39237269
CGTCGTC15750.033.24515563
GTCGTCT15900.033.03224664
TCGTATG1056.4574124E-1032.8478740
GCGTCGT15850.032.71737362
CGTCTCT16550.032.32351766
GGCGTCG16550.031.45810561
TATCAAC23000.031.2298892
TGTGCGA12700.030.82548510
GTGAGTT17000.030.7773751
TGGCGTC16900.030.7007760
Produced by FastQC (version 0.11.5)