FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886858
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29620.3339880792640987No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28070.3165106477023379No Hit
GTAGTACACAGCCGTGTCTTCAGCTCTCAGGCTGTTCATTTGCAGAAACA22490.25359189407999927No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21850.24637540620933676No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCACCTCCTGG21590.24344370801188014No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19390.21863703095647782No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18120.2043168128381319No Hit
GTGTACTACTGTGCGAAAGGTCAGAAGCAATGGCTGGTAGGAAGGTTTGA17870.20149787226365437No Hit
ATATTACTGTCAACAGTATGAAAATCTGCCCCTCACTTTCGGCCCTGGGA17860.20138511464067527No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17390.1960855063606575No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17270.19473241488490828No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16940.19101141332659796No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16890.19044762521170244No Hit
GCTTATGGTGACACTAAAATCTGTCCCAAATCCACTTCCACTGAACCTTG16520.18627559316147568No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16440.18537353217764285No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16420.18514801693168467No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16210.18278010684912352No Hit
GATTTTAGTGTCACCATAAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAGTATGAAAATCTG15990.1802994391435833No Hit
GTATGAAAATCTGCCCCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT15030.16947470733758957No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTACCAACTATTTAAATTGGTATCAGCAGAAA14970.16879816159971495No Hit
AGTCAGGACATTACCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAA14950.16857264635375674No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTACCAA14560.16417509905757177No Hit
GCATGGACATGAGGGTCCCTGCTCACCTCCTGGGGCTCCTGCTGCTCTGG14540.1639495838116136No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14440.16282200758182258No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14170.15977755176138683No Hit
CCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14040.1583117026626585No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTTATGGTGACACTAAAATCTGTCCCAAATCCACTTCCA13930.1570713688098884No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG13670.15413967061243175No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC13620.15357588249753626No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13450.15165900290689152No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTTATGGTGACACTAAAATCTGTCCCAAATCCACTTCCA13430.1514334876609333No Hit
CACTAAAATCTGTCCCAAATCCACTTCCACTGAACCTTGATGGGACCCCT13280.14974212331624678No Hit
ACATATTACTGTCAACAGTATGAAAATCTGCCCCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA13170.14850178946347667No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12940.14590836413495734No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12720.1434276964294171No Hit
GGACATTACCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGTATC12370.13948117962514855No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12360.13936842200216945No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12070.13609845093577552No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGGCATTGCTAC12050.13587293568981731No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11580.13057332740979954No Hit
CAGCATGGACATGAGGGTCCCTGCTCACCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT11280.1271905987204265No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10920.12313132429317883No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCACCTCCTGGGGCTC10840.12222926330934603No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCACCTCCTGGGGCTCCT10770.12143995994849233No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10720.12087617183359681No Hit
GTCCTGACTCGCCTGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCAT10530.11873377699699389No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10510.11850826175103567No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10470.11805723125911927No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10420.11749344314422377No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10410.11738068552124466No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10290.11602759404549545No Hit
GATCTACGATGTATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTG10220.11523829068464174No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10130.11422347207782982No Hit
TTTTCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTTATGGTGACACTAAAATCTGTC10060.1134341687169761No Hit
GTAATGTCCTGACTCGCCTGGCAAGTGATGGTGACTCTGTCTCCTACAGA9970.11241935011016421No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9780.11027695527356127No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9760.11005144002760306No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCACCTCCTGGGGCTCCTGCT9680.10914937904377026No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9620.10847283330589563No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9580.10802180281397923No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9530.10745801469908374No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9440.10644319609227182No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9390.10587940797737631No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9040.10193289117310775No Hit
CCATAAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAG9040.10193289117310775No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG9000.10148186068119135No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCA8960.10103083018927494No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8920.10057979969735854No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCCG150.00246820168.1905065
CGTGTTA200.005959660354.5833866
ACCGTAC2150.047.6257927
CGTATGC2200.047.12587441
GACCGTA2250.045.48598526
GTACTAG2400.044.14922330
CGTACTA2400.044.1217929
GTGGTAT2250.043.9449961
TCGTATG2200.043.93644340
CTAGATC2200.043.59303333
ACGAGAC2450.043.153122
TACTAGA2550.041.5804131
GCCGTCT2300.040.81178346
ATGCCGT2550.040.7904944
ATCTCGT2300.040.3754237
CTTATAC2800.040.1836931
TATACAC2800.040.1836933
GATCTCG2400.040.0783236
AGACCGT2600.039.3584325
TCTCCGA2600.039.3451111
Produced by FastQC (version 0.11.5)