FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954202
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48190.5050293334115837No Hit
GTATTACTGTCAGCGGTATAGTGGCTCACCGACCCCCATGTACACTTTTG37530.3933129463153504No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30120.3156564333338224No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA27770.29102852435857396No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23850.24994707619560638No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22900.23999111299284637No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21780.22825355637485564No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCT19100.20016726018180636No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18940.19849046637923626No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17800.18654331053592424No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17630.18476171712069353No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17310.18140812951555332No Hit
GTGTATTACTGTCAGCGGTATAGTGGCTCACCGACCCCCATGTACACTTT17180.1800457345509651No Hit
GTATAGTGGCTCACCGACCCCCATGTACACTTTTGGCCAGGGGACCAAGC16830.17637774810784299No Hit
CCACTATACCGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTACTGATGGTGAGAGTGAAGTCTGTC15510.16254419923663962No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15490.16233460001131836No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT15120.15845701434287499No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTACTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA14710.1541602302237891No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14370.1505970433933276No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14250.14933944804140004No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGTAGACTGGAGCCTGAAGATT14170.148501051140115No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13940.14609066004892046No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13790.14451866585901096No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13730.1438898681830472No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13680.14336587011974403No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCACAG13670.14326107050708342No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA13400.14043148096524635No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13290.13927868522597942No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13270.13906908600065812No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG13110.13739229219808805No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12960.13582029800817857No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12870.13487710149423288No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC12790.13403870459294784No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12770.13382910536762657No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12060.12638833286872173No Hit
CTCCCAGACTCCTCATCCATGGTGTATCCAGCAGGGCCACTGGCATCCCA11440.11989075688376256No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA11430.11978595727110193No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11420.11968115765844128No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11300.11842356230651371No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11120.11653716927862234No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA10490.10993479368100255No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACATGGGGGT10350.10846759910375373No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTCACAGCAACTACTTATCCTGGTATC10260.10752440258980803No Hit
GTCTGGGAGCCTGGCCAGGTTTCTTCTGATACCAGGATAAGTAGTTGCTG10230.10721000375182614No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT10110.10595240839989856No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAG10110.10595240839989856No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10100.10584760878723794No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9970.1044852138226497No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9890.10364681692136465No Hit
GGCCAAAAGTGTACATGGGGGTCGGTGAGCCACTATACCGCTGACAGTAA9850.10322761847072212No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG9720.10186522350613392No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9690.10155082466815203No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG200.005740689655.102559
GTATCAA17750.038.2109261
ACGGGTT559.159268E-636.47916
GATCAAA18250.032.236370
ATCAACG21500.031.5313553
TATCAAC21550.031.4614962
TCAACGC22000.030.9665324
GTGGTAT2050.030.9663261
CAACGCA22100.030.5257935
AACGCAG22250.030.474896
GCGAACT553.8953032E-430.41991623
CTATAGA450.00517829829.96258731
CGCAGAG23150.029.1657078
AGAGTAC23250.028.61947811
ACGCAGA23750.028.4169647
GCAGAGT24150.027.9594829
AGAGTCG606.5102166E-427.8644411
GCGTAGA16900.025.80138670
CCTATAC650.001046672325.6886773
CCGACAC750.001123954525.37639666
Produced by FastQC (version 0.11.5)