FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906907
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATTCTGGTCATGTATCAAGATAACAAGCGGCCCTCAGGGATCCCT27350.3015744723549383No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC25620.2824986464984833No Hit
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG24870.27422877979770804No Hit
GAATAGGGGACTGGCCTGGCCTCTGTTGATACCAACAAGTATATTTATCC21890.2413698427732943No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20680.22802779116271019No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19730.21755262667506153No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19300.21281123643328367No Hit
CCTATGACCTGACTCAGACACCCGCAGTGTCCGTCTACCCAGGACAGACA19110.21071620353575396No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCC19090.21049567375706657No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16940.1867887225481775No Hit
GTAATAGACGGCCGTGTCCTCGCTTTTCAGGCTGTTCATTTGCAGGTACA16870.1860168683227718No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16810.18535527898670978No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16150.1780777962900275No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT16050.17697514739659084No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15960.1759827633924978No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15950.17587249850315412No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15780.17399799538431174No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14500.15988408954832192No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14460.15944302999094725No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC14160.15613508331063713No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCCCGTGCTGCTGTCCCACACCTGACAGTAATAGTCAG14120.15569402375326247No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC13830.152496341962296No Hit
GGCCAGGCCAGTCCCCTATTCTGGTCATGTATCAAGATAACAAGCGGCCC13740.15150395795820298No Hit
GACTATTACTGTCAGGTGTGGGACAGCAGCACGGGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG13520.14907813039264226No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13460.14841654105658023No Hit
ACCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGAT13400.1477549517205182No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13380.14753442194183086No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13220.14577018371233214No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13180.14532912415495747No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12720.14025693924514862No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12520.1380516414582752No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC12420.13694899256483853No Hit
GTCCATGTAGTGGTCACTGAAGGTGAATCCAGAGGCTACACAGGAGAGTC12070.13308972143781006No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGACTGAACTGGGTTTTC12020.1325383969910917No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11790.13000230453618727No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11780.1298920396468436No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11690.12889965564275058No Hit
GTCCCACACCTGACAGTAATAGTCAGCCTCATCCACAGCCTGGGCCCCGC11330.12493011962637846No Hit
GTAATAGTCAGCCTCATCCACAGCCTGGGCCCCGCTGATGGTCAGAGTGG11250.12404800051162908No Hit
CTATTACTGTCAGGTGTGGGACAGCAGCACGGGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC10860.11974766982722596No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC10690.11787316670838356No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGTTCCGTGG10690.11787316670838356No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10630.11721157737232153No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10310.11368310091332409No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT10090.11125727334776332No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC9940.10960330000760828No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT9770.10772879688876588No Hit
GGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCA9770.10772879688876588No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9700.1069569426633602No Hit
GTATCAACAGAGGCCAGGCCAGTCCCCTATTCTGGTCATGTATCAAGATA9590.10574402888057982No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9550.10530296932320514No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9500.10475164487648678No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9480.10453111509779944No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTCAGTGACCACTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGG9440.10409005554042475No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9260.1021052875322387No Hit
GAGTGGCTGTGTTCTCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT9220.10166422797486402No Hit
CAGCCACTCTGACCATCAGCGGGGCCCAGGCTGTGGATGAGGCTGACTATTACTGTCAGGTGTGGGACAGCAGCA9170.10111290352814567No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC9170.10111290352814567No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9160.10100263863880199No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG9120.10056157908142731No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)