FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906907
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC23550.25967381440434356No Hit
CCCCTATTCTGGTCATGTATCAAGATAACAAGCGGCCCTCAGGGATCCCT23390.25790957617484483No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21960.24214169699869997No Hit
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG21590.2380618960929842No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21100.23265891651514434No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20680.22802779116271019No Hit
GAATAGGGGACTGGCCTGGCCTCTGTTGATACCAACAAGTATATTTATCC20220.22295560625290134No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA17320.19097878834323695No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17080.1883324309989889No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16880.18612713321211546No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCC16870.1860168683227718No Hit
CCTATGACCTGACTCAGACACCCGCAGTGTCCGTCTACCCAGGACAGACA16580.18281918653180537No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15490.1708003135933453No Hit
GTAATAGACGGCCGTGTCCTCGCTTTTCAGGCTGTTCATTTGCAGGTACA14960.16495627445813077No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14940.16473574467944344No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14740.16253044689257004No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14520.16010461932700926No Hit
GGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCA13920.15348872596638907No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13540.1492986601713296No Hit
GGCCAGGCCAGTCCCCTATTCTGGTCATGTATCAAGATAACAAGCGGCCC13400.1477549517205182No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG13350.14720362727379985No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCCCGTGCTGCTGTCCCACACCTGACAGTAATAGTCAG13210.14565991882298845No Hit
ACCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGAT13090.14433674015086442No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12870.14191091258530367No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12770.14080826369186697No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12610.13904402546236824No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC12070.13308972143781006No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12000.13231786721240438No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC11980.13209733743371702No Hit
GACTATTACTGTCAGGTGTGGGACAGCAGCACGGGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG11780.1298920396468436No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11320.12481985473703479No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11300.12459932495834744No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11180.1232761462862234No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11140.12283508672884871No Hit
GTCCCACACCTGACAGTAATAGTCAGCCTCATCCACAGCCTGGGCCCCGC11020.12151190805672467No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10680.11776290181903988No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC10260.11313177646660572No Hit
GTCCATGTAGTGGTCACTGAAGGTGAATCCAGAGGCTACACAGGAGAGTC10050.11081621379038864No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9900.1091622404502336No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGACTGAACTGGGTTTTC9840.10850065111417156No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC9720.10717747244204753No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9720.10717747244204753No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGTTCCGTGG9540.10519270443386146No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9420.10386952576173741No Hit
GTAATAGTCAGCCTCATCCACAGCCTGGGCCCCGCTGATGGTCAGAGTGG9390.10353873109370641No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAC9370.10331820131501906No Hit
GTGTGGGACAGCAGCACGGGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC9280.10232581731092605No Hit
GAGTGGCTGTGTTCTCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT9260.1021052875322387No Hit
GTATCAACAGAGGCCAGGCCAGTCCCCTATTCTGGTCATGTATCAAGATA9240.10188475775355135No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT9210.10155396308552035No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT9120.10056157908142731No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)