FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1303221
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65590.5032914601590981No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55800.4281698959731312No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44390.34061759287181526No Hit
GTAATAAACAGCCGTGTCCGCGGCGGTCACAGAACTCAGCCTCAGGGAGA41000.31460512069710356No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40330.30946401262717527No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGTCTCCTG38260.2935802906797849No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG37660.28897631330372975No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37610.28859264852239186No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36070.27677577325718355No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35140.26963960832429806No Hit
GTTTATTACTGTGCGAGGGCAGGTGCCTACGGGAACGGCGGCTACTACTA34890.26772128441760834No Hit
GATTTTACACTGAGAATCACCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTAGAAACT31730.24347367023705113No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG31020.2380256303420525No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30730.23580037461029252No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG30540.23434244844120836No Hit
GTCTCTGGTGGCTCCGTCAAAAGTAGTAGTTACTACTGGGCCTGGATCCG30300.2325008574907863No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29860.2291246074150125No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28520.21884239127515592No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGGTGATTCTCAGTGTAAAATCTGTGCCTGATCCACTGCCA28460.21838199353755042No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAG28310.2172309991935366No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28070.21538940824311456No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG27920.21423841389910078No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27340.20978790243558076No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26420.20272847045896283No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT26240.2013472772461463No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC26050.19988935107706213No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT25740.19751062943276698No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25150.19298338501297937No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25040.19213932249403592No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23940.18369869730460145No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23420.17970858357868696No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGCAACTGTGGAGACTGCCCTGGCTTCTGCAGGTA23350.17917145288481384No Hit
GGTCTAGTCAGAGTCTCCTGCAAAGTAATGGAAACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC23210.17809719149706765No Hit
GTATTATAGTGGGACCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGGA23020.17663926532798352No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22980.17633233350291316No Hit
CACCTGCACTGTCTCTGGTGGCTCCGTCAAAAGTAGTAGTTACTACTGGG22970.17625560054664557No Hit
AGCCAGGGCAGTCTCCACAGTTGCTGATCTATTTGGGTTCTAATCGGGCC22290.17103775952044972No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGACTGGAGTTT22070.16934963448256282No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21570.16551298666918351No Hit
TTATTACTGCATGCAAGCTCTAGAAACTCCAGTCACTTTCGGCCCTGGGA21560.16543625371291593No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGGACCAGTTCTCCCTGAGG21460.16466892415024006No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21190.16259713433101525No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20990.1610624752056635No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20740.15914415129897386No Hit
GTTTATTACTGCATGCAAGCTCTAGAAACTCCAGTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA20620.15822335582376282No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAGAATCACCAGAG20520.15745602626108696No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG20500.15730256034855178No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG20460.15699562852348145No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20140.1545401739229187No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20090.15415650914158074No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20020.15361937844770762No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA19860.15239165114742625No Hit
GTCTCCACAGTTGCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCC19850.1523149181911587No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC19810.15200798636608834No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19510.14970599767806073No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19500.14962926472179316No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18640.14303023048278074No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGGTGATTCTCAGTGTAAAATCTGTGCCTGATCCACTGCCA18480.14180250318249937No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18150.13927031562566902No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18090.1388099178880635No Hit
GAGAATCACCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTAGAAACTCCAGTCACTTT17990.13804258832538763No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17710.13589406554989522No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC17690.13574059963736004No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17350.13313167912426213No Hit
CTCCACAGTTGCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT17340.13305494616799451No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC17300.1327480143429242No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17200.13198068478024833No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC17090.13113662226130487No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG17060.13090642339250214No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16650.12776037218553107No Hit
GTAGTAGGTGGTCCCACTATAATACATACTTCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGGC16640.12768363922926348No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG16530.12683957671032003No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTTTCCATTACTTTGC16450.12622571306017935No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA16390.12576531532257385No Hit
CTAGAAACTCCAGTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACG16300.12507471871616557No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16290.12499798575989797No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTAGAAACTCCAGTCACTTTCGGCCCTGGGA16210.12438412210975729No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16210.12438412210975729No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16110.12361679254708141No Hit
ACTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTTTCCATTACTTTGCAGGAGACTCTGA16030.12300292889694073No Hit
GTAATGGAAACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGTTGCTGATCTATTTGGGTT15850.12162173568412418No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT15760.12093113907771591No Hit
CTACTACTCCGGTATGGACGTCTGGGGCCAAGGGATCACGGTCACCGTCT15760.12093113907771591No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCA15740.12077767316518073No Hit
CTATAATACATACTTCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGGCCCAGTAGTAACTACTA15720.12062420725264557No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15670.12024054247130762No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15610.1197801447337021No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC15560.11939647995236417No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15540.11924301403982902No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15460.11862915038968831No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15110.11594349692032281No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14890.11425537188243591No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGTTGCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGA14800.11356477527602762No Hit
GAGTAGTAGTAGCCGCCGTTCCCGTAGGCACCTGCCCTCGCACAGTAATA14770.11333457640722486No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG14540.11156971841307038No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14520.1114162525005352No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14390.11041872406905659No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14290.10965139450638073No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14110.10827020129356418No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG13910.10673554216821245No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13880.1065053432994097No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13790.10581474669300141No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG13790.10581474669300141No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13730.10535434895539592No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13700.10512415008659315No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13550.10397315574257936No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG13520.10374295687377659No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13510.10366622391750899No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13480.10343602504870625No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13420.10297562731110073No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13340.10236176366096005No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13270.10182463296708694No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC13230.1015177011420166No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13150.10090383749187588No Hit
CTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTTTC13110.10059690566680554No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)