FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1303221
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64670.49623202818248013No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49270.37806327553039737No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44550.3418453201720967No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42610.32695912665618493No Hit
GTAATAAACAGCCGTGTCCGCGGCGGTCACAGAACTCAGCCTCAGGGAGA39710.3047065693385849No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37990.2915085008605601No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37890.29074117129788424No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGTCTCCTG36550.28045895515802766No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34450.2643450343418346No Hit
GTTTATTACTGTGCGAGGGCAGGTGCCTACGGGAACGGCGGCTACTACTA32840.2519910283827532No Hit
GTCTCTGGTGGCTCCGTCAAAAGTAGTAGTTACTACTGGGCCTGGATCCG31660.24293653954317804No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG31480.24155534633036146No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31100.2386394939921932No Hit
GATTTTACACTGAGAATCACCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTAGAAACT30530.23426571548494077No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30470.23380531774733526No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG30150.2313498631467725No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29670.22766668124592834No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29270.22459736299522493No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGGTGATTCTCAGTGTAAAATCTGTGCCTGATCCACTGCCA28720.22037705040050767No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28200.2163869366745932No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26770.20541412392832836No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT25480.19551557256980973No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAG25130.19282991910044422No Hit
GGTCTAGTCAGAGTCTCCTGCAAAGTAATGGAAACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC25050.1922160554503035No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24650.18914673719960007No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT24640.18907000424333248No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24490.1879190098993187No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24330.1866912825990373No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC23920.18354523139206627No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT23660.18155017452910904No Hit
GTATTATAGTGGGACCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGGA23460.1800155154037573No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC23100.1772531289781242No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22710.17426054368368835No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22510.1727258845583366No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22240.17065409473911178No Hit
AGCCAGGGCAGTCTCCACAGTTGCTGATCTATTTGGGTTCTAATCGGGCC22070.16934963448256282No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGACTGGAGTTT21780.16712437875080283No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGCAACTGTGGAGACTGCCCTGGCTTCTGCAGGTA21490.1648991230190428No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21300.1634411968499587No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21200.1626738672872828No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA20970.16090900929312835No Hit
CACCTGCACTGTCTCTGGTGGCTCCGTCAAAAGTAGTAGTTACTACTGGG20840.15991148086164972No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20830.15983474790538213No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG20630.1583000887800304No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGGACCAGTTCTCCCTGAGG20450.15691889556721383No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20250.15538423644186214No Hit
GTTTATTACTGCATGCAAGCTCTAGAAACTCCAGTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA20170.15477037279172146No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20030.15369611140397524No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAGAATCACCAGAG19820.15208471932235593No Hit
GTCTCCACAGTTGCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCC19690.1510871908908773No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG19670.15093372497834212No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19630.1506267931532718No Hit
TTATTACTGCATGCAAGCTCTAGAAACTCCAGTCACTTTCGGCCCTGGGA19600.15039659428446903No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19550.15001292950313108No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19520.14978273063432834No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18920.14517875325827315No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18140.13919358266940143No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC17950.1377356565003173No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17770.13635446328750073No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGGTGATTCTCAGTGTAAAATCTGTGCCTGATCCACTGCCA17610.13512673598721936No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC17480.13412920755574073No Hit
GAGAATCACCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTAGAAACTCCAGTCACTTT17330.13297821321172695No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17130.1314435540863752No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17050.13082969043623452No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16530.12683957671032003No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16340.1253816505412359No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16290.12499798575989797No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG16040.12307966185320832No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTAGAAACTCCAGTCACTTTCGGCCCTGGGA15910.1220821334217297No Hit
CTCCACAGTTGCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT15890.12192866750919452No Hit
CTAGAAACTCCAGTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACG15800.12123807090278625No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC15790.12116133794651865No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15720.12062420725264557No Hit
GTAGTAGGTGGTCCCACTATAATACATACTTCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGGC15630.11993361064623728No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15550.1193197469960966No Hit
GTAATGGAAACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGTTGCTGATCTATTTGGGTT15370.11793855378328004No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15320.1175548890019421No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTTTCCATTACTTTGC15240.11694102535180141No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15180.11648062761419592No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15100.11586676396405521No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15040.1154063662264497No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15000.11509943440137936No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG14910.11440883779497107No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT14870.11410190596990073No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCA14840.11387170710109797No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14750.11318111049468971No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14650.11241378093201383No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA14650.11241378093201383No Hit
ACTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTTTCCATTACTTTGCAGGAGACTCTGA14620.11218358206321108No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC14530.1114929854568028No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14430.11072565589412695No Hit
CTACTACTCCGGTATGGACGTCTGGGGCCAAGGGATCACGGTCACCGTCT14230.10919099676877521No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14190.10888406494370487No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13890.10658207625567728No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGTTGCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGA13880.1065053432994097No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG13870.1064286103431421No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG13800.105891479649269No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13790.10581474669300141No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT13760.10558454782419865No Hit
CTATAATACATACTTCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGGCCCAGTAGTAACTACTA13650.1047404853052552No Hit
GAGTAGTAGTAGCCGCCGTTCCCGTAGGCACCTGCCCTCGCACAGTAATA13640.10466375234898763No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC13550.10397315574257936No Hit
GGTGATTCTCAGTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGT13240.10159443409828417No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)