FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419585
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16470.3925307148730293No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16020.38180583195300116No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13060.31125993541237174No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13030.3105449432177032No Hit
AGTCAGGACAGTCGCAGAAATTTAAATTGGTATCAGCAAAAACCAGGGAA12730.30339502127101775No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12040.2869502007936413No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11580.275986987142057No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11300.26931372665848397No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9990.23809240082462435No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9820.23404077838816928No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGG9720.23165747107260745No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9390.2237925569312535No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9100.21688096571612428No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG8640.20591775206453997No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7890.18804294719782644No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7860.18732795500315788No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7850.1870896242716017No Hit
ATATTACTGTCAACAGTATGCTAATCTCCCGCTCACTTTCGGCGGAGGGA7810.18613630134537698No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7770.18518297841915227No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAATCACCATCACTT7380.17588807988846122No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7050.16802316574710724No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6580.15682162136396677No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6510.15515330624307352No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6460.1539616525852926No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6380.15205500673284317No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6230.14848004575950044No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG6170.14705006137016338No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA6090.14514341551771393No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC5810.1384701550341409No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC5560.13251188674523634No Hit
ACATATTACTGTCAACAGTATGCTAATCTCCCGCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA5550.13227355601368018No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5440.1296519179665622No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5440.1296519179665622No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5310.12655361845633184No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTG5220.12440864187232624No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTACTGATGGTGAAAGTAAAATCTGTCCCAGACCCACTTCCA5190.12369364967765768No Hit
GTCCTGACTCGCCTGGCAAGTGATGGTGATTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCAT5190.12369364967765768No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5140.12250199601987678No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT5140.12250199601987678No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC5080.1210720116305397No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC5050.12035701943587115No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC4950.11797371212030935No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT4900.11678205846252845No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT4830.11511374334163518No Hit
ATGCTAATCTCCCGCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAA4790.11416042041541047No Hit
GTGGAAGTGGGTCTGGGACAGATTTTACTTTCACCATCAGTAGCCTGCAGCCTGAAGATATTGCAACATATTA4760.11344542822074194No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTACTGATGGTGAAAGTAAAATCTGTCCCAGACCCACTTCCA4710.11225377456296103No Hit
GTATGCTAATCTCCCGCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT4680.11153878236829248No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA4650.11082379017362395No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG4640.11058545944206774No Hit
GTAGGAGACAGAATCACCATCACTTGCCAGGCGAGTCAGGACAGTCGCAGAAATTTAAATTGGTATCAGCAAAAA4630.11034712871051157No Hit
GTCTGCATCTGTAGGAGACAGAATCACCATCACTTGCCAGGCGAGTCAGG4620.1101087979789554No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG4600.10963213651584303No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT4580.10915547505273068No Hit
TCAGTAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAGTAT4580.10915547505273068No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC4550.10844048285806214No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC4510.1074871599318374No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA4510.1074871599318374No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG4490.10701049846872504No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG4490.10701049846872504No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT4480.10677216773716887No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG4460.10629550627405651No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA4440.10581884481094415No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT4430.10558051407938797No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC4290.10224388383760143No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC4260.1015288916429329No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGCGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC4250.10129056091137671No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG350.005580766655.4833870
CGCTCAG200.00590779654.69857862
TCGATCA200.00803359850.60890217
GAAGGAT650.001279399339.8342270
GTATCAA16850.036.842881
TACGATG4650.036.1934670
CGCCTAC903.3560354E-936.0613759
CTTATAA951.9989116E-434.0687470
CCGCCTA955.8935257E-934.04191658
CCGCATT601.5552443E-533.75939621
CTTAGAC400.00290262433.7191663
GTAAAAC601.567995E-533.7191663
CAGTTCG4350.032.7373270
CGTTCAT450.003577622132.30986861
CGCATTT652.6863978E-531.16623722
CCTACCT1051.3975296E-831.15594361
ATCAACG19750.031.072853
TCAACGC19900.030.8386334
TATCAAC19750.030.7387162
TACGGGC1650.030.66474715
Produced by FastQC (version 0.11.5)