FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1642705
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATGGGTAGTGGCATTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC130390.793751769185581No Hit
CCCATATACAGTACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTC95480.5812364362438782No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC70040.42636992034479715No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAATGCCACTACCCATATACAGTACA53300.32446483087346784No Hit
GTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG49130.29907987130982133No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAATGCCACTACCCATATACAGTACACAGTAATAAT48200.29341847745030303No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG47370.2883658356186899No Hit
GATTATTACTGTGTACTGTATATGGGTAGTGGCATTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGT46250.2815478129061517No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG45510.27704304789965334No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42780.26042411753784156No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT42010.25573672692297156No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAACTCTGG38620.23510003317698552No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC38140.2321780234430406No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC36910.22469037349980675No Hit
GTACTGTATATGGGTAGTGGCATTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGG35830.21811585159843064No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC35530.21628959551471508No Hit
GTAGTACAGAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA35480.21598521950076244No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG35330.21507209145890469No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA35240.21452421463379No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT35030.2132458353751891No Hit
CTACTAGTTACTCCCCCACCTGGTACCAGCAGACCCCGGGCCAGACTCCACGCACGCTCATCTACGGCACAAACA34570.2104455760468252No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAT33800.20575818543195523No Hit
CCCCAGAAGAGCGAGTGTTTGTGCCGTAGATGAGCGTGCGTGGAGTCTGG33210.2021665484673146No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT32820.19979241555848434No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGG32290.19656602981058682No Hit
ATACAGTACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGA32000.19480064892966176No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG31590.19230476561525045No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG31560.19212214000687888No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG31230.19011325831479176No Hit
CAGTAGTACAGAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA30880.18798262621712358No Hit
GTCTCTACTAGTTACTCCCCCACCTGGTACCAGCAGACCCCGGGCCAGAC30810.18755649979758995No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG30390.18499974128038815No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC29570.18000797465156554No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28930.17611196167297233No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA28840.17556408484785763No Hit
CTCATCTACGGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAG27650.16831993571578585No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA27500.16740680767392807No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27450.1671024316599755No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG27390.16673718044323235No Hit
ACCCATATACAGTACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTT26810.16320641868138225No Hit
AGTAGAGACTGAGCCAGAGTTCAAGCCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGGCTC26600.16192803942278133No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26040.15851902806651225No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGG25960.1580320264441881No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC25640.15608401995489146No Hit
GTCCTGTGCAGCGTCCGGATTCACCTTCAGTGGCTCTGGCTTCCACTGGGTCCGCCAGGCTCCAGGCAAGGGG25300.15401426306001384No Hit
GTACTACTGTGCGAGAGCGGTGGGAGTTGTTCCGGGGTACTTTGACAACTGGGGCCAGGGAACCCTGGTCACCGT25170.15322288542373705No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24890.15151837974560253No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24740.15060525170374472No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT24620.1498747492702585No Hit
ACTGTGTACTGTATATGGGTAGTGGCATTTGGGTGTTCGGCGGAGGGACC24260.14768324196979982No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT23750.1445786066274833No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23620.14378722899120658No Hit
GATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACA23160.1409869696628427No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT23150.14092609446005216No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA23120.14074346885168063No Hit
AACTAGTAGAGACTGAGCCAGAGTTCAAGCCACAAGTGAGTGTGACTGTC22820.13891721276796504No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22210.13520382539774337No Hit
GTAGATGAGCGTGCGTGGAGTCTGGCCCGGGGTCTGCTGGTACCAGGTGG22190.13508207499216232No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT21670.1319165644470553No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21510.130942561202407No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATT21500.1308816859996165No Hit
CCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAACTCTGGCTCAGTC21430.13045555958008287No Hit
CTGTACTACTGTGCGAGAGCGGTGGGAGTTGTTCCGGGGTACTTTGACAA21420.13039468437729235No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACAC21250.1293598059298535No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAATGCCACTAC20990.1277770506573No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC20550.12509854173451715No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20310.1236375368675447No Hit
CTAGTAGAGACTGAGCCAGAGTTCAAGCCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGG20300.12357666166475417No Hit
CACTTGTGGCTTGAACTCTGGCTCAGTCTCTACTAGTTACTCCCCCACCTGGTACCAGCAGACCCCGGGCCAGA20190.12290703443405845No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC19840.12077640233639028No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC19840.12077640233639028No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAG19560.11907189665825574No Hit
GGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTC19400.11809789341360744No Hit
GTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATC19070.1160890117215203No Hit
CTCCACGCACGCTCATCTACGGCACAAACACTCGCTCTTCTGGGGTCCCT19000.11566288530198666No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT18940.11529763408524354No Hit
CCCAAATGCCACTACCCATATACAGTACACAGTAATAATCAGATTCATCA18840.11468888205733835No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGG18650.11353225320431849No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18380.11188862272897447No Hit
CATCTACGGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGC18230.11097549468711666No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18080.11006236664525888No Hit
CCTTGGTCCCTCCGCCGAACACCCAAATGCCACTACCCATATACAGTACACAGTAATAATCAGATTCATCATCTG17880.1088448625894485No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG17790.10829698576433383No Hit
GTATTGATGACTTCCATCATGCCATATAACTGCCACCCACTCCAGCCCCT17680.10762735853363811No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17380.10580110244992254No Hit
CACCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTACTGTGTAC17210.1047662240024837No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG17190.10464447359690267No Hit
GTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGA17160.10446184798853111No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC17150.10440097278574059No Hit
GCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCC17120.10421834717736904No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17100.10409659677178801No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA17040.10373134555504487No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC17000.10348784474388281No Hit
CCCTGAGACTGTCCTGTGCAGCGTCCGGATTCACCTTCAGTGGCTCTGGC16890.1028182175131871No Hit
ACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACC16700.10166158866016722No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG16440.10007883338761372No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG150.002140025170.6911554
AGGGGGG1850.055.92370670
GGTATCA9050.049.1914981
GGATGGG12650.045.2547370
TGGTATC2000.039.0809562
CGTATGC1750.037.45266741
TCGTATG1850.037.23645840
GTATCAA22100.036.1362341
GTGGTAT2200.035.5281451
CCGTCAG400.00230584935.34557754
TATACAC2600.035.2904933
TCTGGAG15850.034.81265670
ACGAGAC2250.034.7428622
CTTATAC2650.033.3422431
TCTCGTA1650.033.2808738
CGAGACA2700.032.73077423
TCCGAGA11400.032.6712270
ATCTCGT1800.032.37436337
ATGCCGT2200.031.5088544
TTATACA2700.031.466152
Produced by FastQC (version 0.11.5)