FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1163908
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40450.34753605955109856No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37530.3224481660062479No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31680.27218646147289993No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28060.2410843468727769No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23390.20096090068974526No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23040.197953790162109No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22160.19039305512119514No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22070.1896197981283744No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21920.18833103647367316No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21920.18833103647367316No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21740.1867845224880317No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGCGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA21270.1827464026366345No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21250.182574567749341No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGACCATTGGAAGTTATTTACATTGGTATCAGCAGAAA21240.18248865030569425No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20850.17913787000347106No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18840.16186846383047457No Hit
GTAGTAAGTTGCAGCATCCTCAGGCTGCAGACTGGTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA18640.1601501149575396No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18430.15834584864095785No Hit
AGTCAGACCATTGGAAGTTATTTACATTGGTATCAGCAGAAACCAGGAAAAGCCCCTAAGTTCCTGATCTATG18060.1551669032260282No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18040.15499506833873467No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17940.1541358939022672No Hit
GAACAGGGGAGGTGCCTGTATGTGAGCAGCAGTAATAATCAGCTTCATCC17880.1536203892403867No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGATCCAGTC17460.15001185660722324No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG16940.14554414953759232No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16600.14262295645360287No Hit
GGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC16410.14099052502431464No Hit
CACCATCACCAGTCTGCAGCCTGAGGATGCTGCAACTTACTACTGTCAACAGAGTTATCGGCCCCCCCTGACATT16040.13781157960938492No Hit
ATCCAGTCCCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCAT15870.1363509830673902No Hit
ACTTACTACTGTCAACAGAGTTATCGGCCCCCCCTGACATTCGGCCACGGGACCAGGGTGGAAATCAAACGAA15790.13566364351821622No Hit
CCCCTGTTCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC15760.13540589118727597No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCGTGGCCGAATGTC15400.132312863215993No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15220.13076634923035155No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15050.1293057526883568No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15020.12904800035741656No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14650.12586905494248687No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14590.12535355028060638No Hit
GTCCCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTT14470.12432254095684539No Hit
GCATAGATCAGGAACTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAATG14140.12148726531650267No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14060.1207999257673287No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14030.12054217343638846No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13970.12002666877450796No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT13960.1199407513308612No Hit
CCCTGGTCCCGTGGCCGAATGTCAGGGGGGGCCGATAACTCTGTTGACAGTAGTAAGTTGCAGCATCCTCAGG13750.11813648501427948No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13680.11753506290875225No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13510.11607446636675751No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGAACAGGGGAGGTGCCTGTATGTGAGCAG13500.11598854892311077No Hit
GTGTCCGGGTCTCCCGGACAGTCAGTCACCATCTCCTGCACTGGAATCAG13480.11581671403581727No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCC13290.11418428260652903No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13260.11392653027558879No Hit
GATTTCACTCTCACCATCACCAGTCTGCAGCCTGAGGATGCTGCAACTTACTACTGTCAACAGAGTTATCGGCCC13200.11341102561370829No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12920.11100533719159934No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACGCCA12870.11057574997336558No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12780.10980249298054486No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12200.10481928124903342No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGA12060.10361643703797896No Hit
TCCTCAGGCTGCAGACTGGTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACG11840.10172625327775048No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11800.10138258350316348No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11700.10052340906669599No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11670.10026565673575574No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11650.10009382184846226No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAT200.006699463452.99349251
GCCGTTA200.00802508750.62815515
CGTATGC1600.045.3576841
ACGAGAC1850.043.79968622
GTGGTAT3250.043.6218531
TCGTATG1750.041.3679940
CGAGACT2050.039.5316423
ATCTCGT1500.038.8295437
TCTCCGA2100.038.5771511
TCCGAGC2100.038.57383313
ATCTCCG2150.037.68000810
CCCACGA2150.037.67838719
GTATCAA34150.037.659321
GTATGCC2350.036.8402442
CACGAGA2200.036.82680521
CCGTCTT2000.036.7157247
ATGCCGT2100.036.43302544
GCCCACG2400.035.15844318
GCTAATC1850.034.96624833
CTTATAC2550.033.0931431
Produced by FastQC (version 0.11.5)