FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1163908
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39630.34049082917206513No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29570.25405788086343595No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29240.25122260522309325No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28310.24323228296394558No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22530.19357200053612486No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21590.18549576083333047No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21230.18240273286204753No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21150.18171539331287354No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20040.1721785570680844No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19940.17131938263161695No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19620.16857002443492097No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGCGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA19370.16642208834375224No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGACCATTGGAAGTTATTTACATTGGTATCAGCAGAAA19310.16590658368187175No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18940.16272763826694206No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18930.1626417208232953No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18890.16229805104870831No Hit
GTAGTAAGTTGCAGCATCCTCAGGCTGCAGACTGGTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA17930.15404997645862042No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17690.15198795781109847No Hit
GAACAGGGGAGGTGCCTGTATGTGAGCAGCAGTAATAATCAGCTTCATCC16500.14176378201713538No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16500.14176378201713538No Hit
AGTCAGACCATTGGAAGTTATTTACATTGGTATCAGCAGAAACCAGGAAAAGCCCCTAAGTTCCTGATCTATG16450.14133419479890164No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16090.1382411668276187No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16000.13746790983479795No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15760.13540589118727597No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15560.133687542314341No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15510.13325795509610724No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCGTGGCCGAATGTC15410.13239878065963978No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGATCCAGTC15410.13239878065963978No Hit
ACTTACTACTGTCAACAGAGTTATCGGCCCCCCCTGACATTCGGCCACGGGACCAGGGTGGAAATCAAACGAA15300.13145368877952554No Hit
GGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC15260.13111001900493852No Hit
ATCCAGTCCCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCAT15130.1299930922375308No Hit
CCCCTGTTCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC14770.12690006426624786No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14750.12672822937895434No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14530.12483804561872588No Hit
CACCATCACCAGTCTGCAGCCTGAGGATGCTGCAACTTACTACTGTCAACAGAGTTATCGGCCCCCCCTGACATT14210.12208868742202993No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14010.12037033854909494No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13970.12002666877450796No Hit
GTCCCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTT13520.11616038381040425No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13450.11555896170487702No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13420.11530120937393677No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13220.1135828605010018No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13110.11263776862088755No Hit
GCATAGATCAGGAACTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAATG12980.11152084185347982No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACGCCA12960.11134900696618633No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12710.10920107087501763No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT12600.10825597899490338No Hit
GATTTCACTCTCACCATCACCAGTCTGCAGCCTGAGGATGCTGCAACTTACTACTGTCAACAGAGTTATCGGCCC12300.10567845568550092No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12180.10464744636173993No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12020.10327276726339195No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11850.10181217072139721No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGAACAGGGGAGGTGCCTGTATGTGAGCAG11830.10164033583410373No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11810.10146850094681023No Hit
GTGTCCGGGTCTCCCGGACAGTCAGTCACCATCTCCTGCACTGGAATCAG11800.10138258350316348No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCC11730.10078116139763624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3300.051.066631
GTATCAA33050.039.0578651
ATCAACG37850.033.9820253
TAACCCA850.00284240933.8654870
TCAACGC38000.033.843524
CAACGCA39650.032.604975
AACGCAG40450.031.9601256
AATGGTA7250.031.7634870
TATCAAC40700.031.6974352
CGCAGAG41850.030.9714158
ACGCAGA42000.030.940967
TTAACGG450.0048722130.33881835
AGAGTAC42600.030.10873411
TTCGGAC450.005206849429.9294853
GCAGAGT44750.028.8890979
TGGTCAG11750.028.78565470
CGAGGGA500.00650930128.59265556
CCACGTA500.006649690728.46800654
GAGTTTA3200.028.4377331
GACAGGT2500.028.3113421
Produced by FastQC (version 0.11.5)