FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259217
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52980.4207376488722754No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48150.38238047929784935No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG39730.3155135294393262No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC39590.31440172742267614No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37210.2955010931396257No Hit
CTTCTGCATCCACCTTGCAAAGGGGGGTCCCATCACGGTTCAGTGGCAGCGGATCTGGGACAGATTTCACTTTGA34600.2747739269720787No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34350.27278856622806075No Hit
GTCTCCACCCTCCCTGTCTGCATCTTTAGGTGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGGCCA33920.26937374574835No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTCAAAGTGAAATCTGTCCCAGATCCGCTGCCA31920.25349085979620667No Hit
GTTTGGGGCTTTGCCTGGTGTCTGCTGAAACCAAGCCAAAAAGTGGCCAA31490.25007603931649586No Hit
ATGCAGAAGAGATCACGAGGTTTGGGGCTTTGCCTGGTGTCTGCTGAAACCAAGCCAAAAAGTGGCCAATGCT31310.248646579580803No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30990.24610531782846007No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT30780.24443761480348503No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30660.24348464164635641No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30460.2418963530511421No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30410.24149928090233852No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG29490.23419315336435262No Hit
ACTTACTACTGTCTACAAAATTACATTGTCCCGTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAA29190.23181072047153115No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29060.23077833288464178No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC28910.22958711643823104No Hit
GCCCCAAACCTCGTGATCTCTTCTGCATCCACCTTGCAAAGGGGGGTCCCATCACGGTTCAGTGGCAGCGGAT28130.22339279091689515No Hit
CTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCA27150.21561017680034494No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26620.211401212023027No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT26530.21068648215518057No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT25520.20266562474934824No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25000.19853607440179097No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24550.19496242506255873No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24460.19424769519471227No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23770.18876809954122284No Hit
AGTCAGAGCATTGGCCACTTTTTGGCTTGGTTTCAGCAGACACCAGGCAAAGCCCCAAACCTCGTGATCTCTT23600.18741805423529065No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23050.18305026059845128No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22840.1813825575734762No Hit
GTCCCGTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC22400.1778883226640047No Hit
ATGTAATTTTGTAGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTCAAAGTGAAATCTGTC21870.17367935788668673No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21820.17328228573788315No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG21280.16899390653080446No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTG21030.16700854578678656No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21020.16692913135702583No Hit
GTTCAGTGGCAGCGGATCTGGGACAGATTTCACTTTGACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA21010.1668497169272651No Hit
GTACGGGACAATGTAATTTTGTAGACAGTAGTAAGTTGCAAAATCTTCAG19190.15239629071081473No Hit
ACATTGTCCCGTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGA18860.1497756145287111No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18710.14858439808230034No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT18680.14834615479301821No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCACCCTCC18300.14532840646211098No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18090.14366070343713594No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT17760.1410400272550323No Hit
GGCCAAAAGTGTACGGGACAATGTAATTTTGTAGACAGTAGTAAGTTGCA17290.13730754905627862No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17240.13691047690747504No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17240.13691047690747504No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17200.1365928191884322No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17170.13635457589915004No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA16800.13341624199800353No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT16620.13198678226231064No Hit
AAATTACATTGTCCCGTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCAT16530.13127205239446418No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCC16420.1303984936670963No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC16010.1271425020469069No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15620.12404533928623898No Hit
GTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCTGTAGCACTGTC15370.12205997854222107No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC15250.12110700538509249No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15230.12094817652557105No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15200.12070993323628891No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAA15090.11983637450892103No Hit
ACCCAGTCTCCACCCTCCCTGTCTGCATCTTTAGGTGACAGAGTCACCAT15060.11959813121963887No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGT14950.11872457249227099No Hit
GTCTGCATCTTTAGGTGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA14860.11800984262442454No Hit
CTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAACATCCGAATTATTGTTCCT14850.11793042819466383No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14720.11689804060777452No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14530.11538916644232089No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14370.11411853556614945No Hit
CCACTGAACCGTGATGGGACCCCCCTTTGCAAGGTGGATGCAGAAGAGAT14140.11229200368165296No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14000.11118020166500293No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13990.11110078723524221No Hit
GTCCCATCACGGTTCAGTGGCAGCGGATCTGGGACAGATTTCACTTTGAC13970.1109419583757208No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCACCCTCCCTGTCTGCATCTTTAGGTGACAGAGTCACCATCA13920.1105448862269172No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13860.1100683996483529No Hit
TTTCTGGGGAGTCCTGGCGGTTTTTATTAAGGCTGTTCATGTGAAAGCCC13770.10935366978050645No Hit
GATCTGGGACAGATTTCACTTTGACCATCAGCAGTCTGCAACCTGAAGAT13760.10927425535074574No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13560.10768596675553141No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13500.1072094801769671No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCTACAAAATTACATTGTCCCGTACACTTTTGGCCAGGGGA13410.10649475030912066No Hit
GTGATGGGACCCCCCTTTGCAAGGTGGATGCAGAAGAGATCACGAGGTTT13390.10633592144959925No Hit
GTGTAGCAGGTCTCTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAG13230.10506529057342777No Hit
CTTTTTGGCTTGGTTTCAGCAGACACCAGGCAAAGCCCCAAACCTCGTGA13190.10474763285438492No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13050.10363583083773488No Hit
CCACCCTCCCTGTCTGCATCTTTAGGTGACAGAGTCACCATCACTTGCCG12910.10252402882108486No Hit
CAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTCAAAGTGAAATCTGTCCCAGATCCGCTGCCA12900.10244461439132414No Hit
GATTTCACTTTGACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCTACAAAATTACATTGTC12850.10204754224252055No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12840.10196812781275984No Hit
CACCATCACTTGCCGGGCAAGTCAGAGCATTGGCCACTTTTTGGCTTGGT12780.10149164123419553No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12700.10085632579610981No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12670.10061808250682765No Hit
GCTCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCACCTAAAGATGCAGACAGGGAGGGTGGAGACTGGGTCAT12640.10037983921754551No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)