FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259217
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52190.41446390892117874No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTCAAAGTGAAATCTGTCCCAGATCCGCTGCCA43040.3417997056901233No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38990.3096368616370332No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37750.29978947234670433No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37700.29939240019790075No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC37280.29605699414795067No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG37020.29399221897417205No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36640.2909744706432648No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32890.2611940594829962No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT32340.25682626584615675No Hit
GTTTGGGGCTTTGCCTGGTGTCTGCTGAAACCAAGCCAAAAAGTGGCCAA31400.24936130944864945No Hit
ATGCAGAAGAGATCACGAGGTTTGGGGCTTTGCCTGGTGTCTGCTGAAACCAAGCCAAAAAGTGGCCAATGCT29360.23316076577746328No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29070.23085774731440253No Hit
CTTCTGCATCCACCTTGCAAAGGGGGGTCCCATCACGGTTCAGTGGCAGCGGATCTGGGACAGATTTCACTTTGA28900.22950770200847034No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28220.22410752078474164No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27950.22196333118120226No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27710.2200573848669451No Hit
ACTTACTACTGTCTACAAAATTACATTGTCCCGTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAA27650.2195808982883808No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27230.21624549223843073No Hit
GTCTCCACCCTCCCTGTCTGCATCTTTAGGTGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGGCCA27170.2157690056598664No Hit
CTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCA26880.21346598719680565No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC25780.20473039992312683No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG25670.20385684119575898No Hit
ATGTAATTTTGTAGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTCAAAGTGAAATCTGTC25630.2035391834767161No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24310.19305647874830154No Hit
GTACGGGACAATGTAATTTTGTAGACAGTAGTAAGTTGCAAAATCTTCAG24120.19154760458284792No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23700.1882121985328978No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTG23480.18646508107816206No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23190.1841620626151013No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22530.178920710250894No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22470.1784442236723297No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22420.17804715152352613No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT22140.17582354749022608No Hit
GCCCCAAACCTCGTGATCTCTTCTGCATCCACCTTGCAAAGGGGGGTCCCATCACGGTTCAGTGGCAGCGGAT22060.17518823205214035No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21770.17288521358907957No Hit
GTCCCGTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC21750.17272638472955812No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20640.16391138302611863No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20560.16327606758803287No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG20420.16216426557138286No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19840.1575582286452613No Hit
GGCCAAAAGTGTACGGGACAATGTAATTTTGTAGACAGTAGTAAGTTGCA19410.1541434081655505No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19160.15215804742153258No Hit
GTTCAGTGGCAGCGGATCTGGGACAGATTTCACTTTGACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA18760.14898147023110392No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT18750.14890205580134322No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18030.14318421685857163No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17850.14175475712287874No Hit
AGTCAGAGCATTGGCCACTTTTTGGCTTGGTTTCAGCAGACACCAGGCAAAGCCCCAAACCTCGTGATCTCTT17720.14072236953598943No Hit
ACATTGTCCCGTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGA17130.13603691818010716No Hit
CAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTCAAAGTGAAATCTGTCCCAGATCCGCTGCCA17100.135798674890825No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT17090.1357192604610643No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA16990.13492511616345712No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCACCCTCC16540.13135146682422488No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16430.13047790809685703No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCC16280.12928669165044626No Hit
GTCCCAGATCCGCTGCCACTGAACCGTGATGGGACCCCCCTTTGCAAGGT16200.12865137621236053No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16030.12730133090642837No Hit
AAATTACATTGTCCCGTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCAT15970.12682484432786406No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15970.12682484432786406No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC15920.12642777217906048No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14860.11800984262442454No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14810.11761277047562096No Hit
GTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCTGTAGCACTGTC14770.11729511275657811No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAA14710.1168186261780138No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14600.11594506745064592No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGT14470.11491267986375661No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT14240.11308614797926012No Hit
TTTCTGGGGAGTCCTGGCGGTTTTTATTAAGGCTGTTCATGTGAAAGCCC14120.11213317482213153No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14110.11205376039237082No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13970.1109419583757208No Hit
CTGCTGATGGTCAAAGTGAAATCTGTCCCAGATCCGCTGCCACTGAACCG13820.10975074192931004No Hit
CTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAACATCCGAATTATTGTTCCT13740.10911542649122431No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13740.10911542649122431No Hit
GTGTAGCAGGTCTCTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAG13740.10911542649122431No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCTACAAAATTACATTGTCCCGTACACTTTTGGCCAGGGGA13710.10887718320194216No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC13630.10824186776385643No Hit
GGACAATGTAATTTTGTAGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTCAAAGTGAAAT13620.10816245333409572No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13540.10752713789600998No Hit
GGTTGCAGACTGCTGATGGTCAAAGTGAAATCTGTCCCAGATCCGCTGCC13280.10546236272223136No Hit
CCACTGAACCGTGATGGGACCCCCCTTTGCAAGGTGGATGCAGAAGAGAT13280.10546236272223136No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13260.10530353386270991No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13200.10482704728414563No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13180.10466821842462419No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12860.10212695667228128No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCACCCTCCCTGTCTGCATCTTTAGGTGACAGAGTCACCATCA12850.10204754224252055No Hit
GCTCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCACCTAAAGATGCAGACAGGGAGGGTGGAGACTGGGTCAT12820.1018092989532384No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12800.10165047009371697No Hit
ACCCAGTCTCCACCCTCCCTGTCTGCATCTTTAGGTGACAGAGTCACCAT12750.10125339794491339No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)