FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1231255
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC53960.43825202740293445No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCCCCTGAGAACCATCATAATAGAGCAGG45250.36751119792406933No Hit
GCACTGGACCGGTCACCTTTGGTTACTATCCAGCCTGGTTCCAGCAGAAA45210.3671863261468989No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38770.31488197002245677No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36560.2969328043337895No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCCCCTGAGAACCATCATAATAGAGCAGGCAGTAATAGT33310.27053697243869057No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC33280.27029331860581274No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA31840.25859793462767666No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT31430.25526799891167956No Hit
GACTATTACTGCCTGCTCTATTATGATGGTTCTCAGGGGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT30250.24568428148515134No Hit
CTCATTTACAGTTCAACCAAAAAACACTCGTGGACACCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAGG29020.23569447433716006No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACAGAGTCAGGGCAG29010.23561325639286745No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28980.23536960255998962No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT28680.23293306423121124No Hit
CATTTACAGTTCAACCAAAAAACACTCGTGGACACCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAGGCTGC27650.22456761596907218No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27080.2199381931443933No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26150.2123849243251804No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25880.21019203982927986No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG25810.2096235142192316No Hit
CCTTTGGTTACTATCCAGCCTGGTTCCAGCAGAAACCTGGACAAGCACCC25770.20929864244206114No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25310.205562617004601No Hit
GTCCAGTGCTGGAGGCACAGGTGAGAGTGACTGTCCCTCCTGGGGACACAGTCACTGAGGGCTCCTGAGTCACCA25100.20385704017445616No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24040.19524793807943927No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22850.18558300270861844No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG22690.18428351559993666No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22580.18339011821271792No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22530.18298402849125486No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22280.18095357988393956No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22260.18079114399535434No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22210.18038505427389126No Hit
GGATATACAGTGGCAACGGCAACACAAAATATTCTCAGAAGTTCCAGGGCCGCGTCACCCTCACCAGGGACACGT21970.17843582361086857No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21690.17616172117067544No Hit
GTAAATGAGTGCCCTGGGTGCTTGTCCAGGTTTCTGCTGGAACCAGGCTG21610.17551197761633455No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21590.17534954172774933No Hit
CTCAGTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCCTCCAGCACTGGACCGGTCACCTTTGGTTA21110.17145108040170395No Hit
GTCCAATGCTCAGATCGTGGTGACTCAGGAGCCCTCAGTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC20900.1697455035715591No Hit
CTATTACTGCCTGCTCTATTATGATGGTTCTCAGGGGGCATTCGGCGGAG20340.16519729869117283No Hit
CTCCAGCACTGGACCGGTCACCTTTGGTTACTATCCAGCCTGGTTCCAGC19220.15610088893040028No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAGGCTGCCCTGACTCTGTCAGGTG19170.15569479920893722No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19040.15463896593313325No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18760.15236486349294012No Hit
TCATAATAGAGCAGGCAGTAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACAGAGTCAGGGCAGCCTTGCCC18550.15065928666279527No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18540.15057806871850268No Hit
GCCTGAGGACGAGGCTGACTATTACTGCCTGCTCTATTATGATGGTTCTC17790.14448672289655679No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATGCTCAGAT17650.14334967167646018No Hit
ATTATGATGGTTCTCAGGGGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT17600.14294358195499715No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17270.14026338979334094No Hit
CAGTAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACAGAGTCAGGGCAGCCTTGCCCCCAAGGAGGGAGC16950.13766441557597736No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16870.13701467202163647No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT16870.13701467202163647No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16730.1358776208015399No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16590.13474056958144331No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16330.1326289030298354No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16130.13100454414398316No Hit
CTATTATGATGGTTCTCAGGGGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC16040.13027358264534966No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15850.12873044170379003No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15750.12791826226086392No Hit
CAGTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCCTCCAGC15620.12686242898505995No Hit
GGGCAGCCTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGTGTCCAC15610.12678121104076737No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15520.12605024954213384No Hit
CCTGACAGAGTCAGGGCAGCCTTGCCCCCAAGGAGGGAGCCTGAGAACCG15430.12531928804350034No Hit
AGGCTGCCCTGACTCTGTCAGGTGTGCAGCCTGAGGACGAGGCTGACTATTACTGCCTGCTCTATTATGATGGTT15270.12401980093481853No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCCC15210.12353249326906286No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCCCCTGAGAACCAT14980.12166448055033278No Hit
CCTGAGAACCGGGCAGGTGTCCACGAGTGTTTTTTGGTTGAACTGTAAAT14880.12085230110740668No Hit
CTCTCACCTGTGCCTCCAGCACTGGACCGGTCACCTTTGGTTACTATCCA14750.11979646783160272No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14670.11914672427726183No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14450.11735992950282435No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14420.1171162756699465No Hit
CCTTGGGGGCAAGGCTGCCCTGACTCTGTCAGGTGTGCAGCCTGAGGACG14350.11654775005989823No Hit
GCTTGGTCCCTCCGCCGAATGCCCCCTGAGAACCATCATAATAGAGCAGG14270.11589800650555733No Hit
GCCCTGACTCTGTCAGGTGTGCAGCCTGAGGACGAGGCTGACTATTACTG14190.11524826295121644No Hit
TCCTCAGGCTGCACACCTGACAGAGTCAGGGCAGCCTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGTGTC14090.11443608350829033No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14060.11419242967541249No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13980.1135426861210716No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13900.11289294256673069No Hit
ATCCAGCCTGGTTCCAGCAGAAACCTGGACAAGCACCCAGGGCACTCATT13130.10663916085619955No Hit
CTGTAAATGAGTGCCCTGGGTGCTTGTCCAGGTTTCTGCTGGAACCAGGCTGGATAGTAACCAAAGGTGACCGGT13090.10631428907902911No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13070.10615185319044389No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCCCCTGAGAAC13060.10607063524615129No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12980.10542089169181038No Hit
GTCTGGGGCTGAGGTGAAGACGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCCTCTGGATACACCTTCACGAG12830.1042026225274212No Hit
CTGTATATCCTGCCCATCCACTCAAGAGCCTGTCCGGGGGCCTGGCGCAC12730.10339044308449509No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12530.10176608419864286No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12500.10152243036576501No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG12460.10119755858859457No Hit
GCCTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGTGTCCACGAGTG12350.10030416120137584No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG12340.10022294325708321No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTTCG200.007623729351.28972627
GTACGGT200.00765317751.2397414
GGTATCA12450.048.588251
TCGTATG1650.041.81167240
GTCGTAT350.001354330139.3901239
TCTCGTA1550.037.74304638
GACAGGT1750.037.1058921
ATCTCGT1600.036.5277137
GTGGTAT2650.036.1109961
CGTATGC1950.035.4183241
AGGGGGG3450.035.2414470
TAACCGT601.2076376E-535.0377653
GTAGGTC1004.2382453E-1034.1584420
GTATCAA30650.033.6747061
GCCGTCT2000.032.98633646
TATACAC2650.032.2367253
CGGTACC652.2375498E-532.01845645
CACGAGA2250.031.88250421
CCCACGA2250.031.88120819
CTTATAC2600.031.5475221
Produced by FastQC (version 0.11.5)