FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1231255
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC50700.41177497756354287No Hit
GCACTGGACCGGTCACCTTTGGTTACTATCCAGCCTGGTTCCAGCAGAAA43850.35614068572310364No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCCCCTGAGAACCATCATAATAGAGCAGG40360.32779562316498206No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG38270.31082107280782617No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37530.3048109449301729No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33770.2742729978761508No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA33650.2732983825446394No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC30200.24527819176368826No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29880.24267921754632468No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29580.24024267921754633No Hit
GACTATTACTGCCTGCTCTATTATGATGGTTCTCAGGGGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT28490.23138992328965163No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28340.23017165412526244No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG28130.22846607729511756No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT27860.22627319279921707No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27790.22570466718916876No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCCCCTGAGAACCATCATAATAGAGCAGGCAGTAATAGT27660.22464883391336482No Hit
CTCATTTACAGTTCAACCAAAAAACACTCGTGGACACCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAGG27270.22148133408595294No Hit
CCTTTGGTTACTATCCAGCCTGGTTCCAGCAGAAACCTGGACAAGCACCC26660.21652703948410362No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACAGAGTCAGGGCAG26640.2163646035955184No Hit
CATTTACAGTTCAACCAAAAAACACTCGTGGACACCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAGGCTGC25930.21059812955074295No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25290.2054001811160158No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23480.190699733199053No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23180.18826319487027465No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22600.18355255410130314No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22440.18225306699262134No Hit
GTCCAATGCTCAGATCGTGGTGACTCAGGAGCCCTCAGTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC22360.18160332343828045No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG21640.17575563144921239No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG21520.17478101611770103No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21220.17234447778892267No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21150.1717759521788744No Hit
GGATATACAGTGGCAACGGCAACACAAAATATTCTCAGAAGTTCCAGGGCCGCGTCACCCTCACCAGGGACACGT20780.16877088824004777No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAGGCTGCCCTGACTCTGTCAGGTG20410.1657658243012211No Hit
GTAAATGAGTGCCCTGGGTGCTTGTCCAGGTTTCTGCTGGAACCAGGCTG20220.16422268335966148No Hit
GTCCAGTGCTGGAGGCACAGGTGAGAGTGACTGTCCCTCCTGGGGACACAGTCACTGAGGGCTCCTGAGTCACCA20010.16251710652951662No Hit
CTATTACTGCCTGCTCTATTATGATGGTTCTCAGGGGGCATTCGGCGGAG19990.1623546706409314No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19890.1615424911980053No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19800.1608115296993718No Hit
CTCAGTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCCTCCAGCACTGGACCGGTCACCTTTGGTTA19440.15788768370483774No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19370.15731915809478947No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19200.15593845304181506No Hit
ATTATGATGGTTCTCAGGGGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT17920.14554255617236073No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17830.14481159467372723No Hit
CTCCAGCACTGGACCGGTCACCTTTGGTTACTATCCAGCCTGGTTCCAGC17350.14091313334768182No Hit
TCATAATAGAGCAGGCAGTAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACAGAGTCAGGGCAGCCTTGCCC17060.13855781296319608No Hit
GCCTGAGGACGAGGCTGACTATTACTGCCTGCTCTATTATGATGGTTCTC16960.13774563352026997No Hit
GGGCAGCCTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGTGTCCAC16800.13644614641158817No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16700.13563396696866206No Hit
CAGTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCCTCCAGC16630.13506544135861379No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16360.13287255686271326No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16330.1326289030298354No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16200.13157306975403146No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16170.1313294159211536No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15960.12962383909100877No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15750.12791826226086392No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATGCTCAGAT15740.1278370443165713No Hit
CAGTAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACAGAGTCAGGGCAGCCTTGCCCCCAAGGAGGGAGC15640.12702486487364517No Hit
CTATTATGATGGTTCTCAGGGGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC15620.12686242898505995No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15190.12337005738047764No Hit
AGGCTGCCCTGACTCTGTCAGGTGTGCAGCCTGAGGACGAGGCTGACTATTACTGCCTGCTCTATTATGATGGTT15150.1230451856033072No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14950.12142082671745494No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC14790.12012133960877316No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCCC14430.11719749361423913No Hit
CCTGACAGAGTCAGGGCAGCCTTGCCCCCAAGGAGGGAGCCTGAGAACCG14200.11532948089550904No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14160.1150046091183386No Hit
CCTTGGGGGCAAGGCTGCCCTGACTCTGTCAGGTGTGCAGCCTGAGGACG14090.11443608350829033No Hit
GCTTGGTCCCTCCGCCGAATGCCCCCTGAGAACCATCATAATAGAGCAGG13800.11208076312380456No Hit
CTCTCACCTGTGCCTCCAGCACTGGACCGGTCACCTTTGGTTACTATCCA13660.11094371190370801No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13460.10931935301785577No Hit
TCCTCAGGCTGCACACCTGACAGAGTCAGGGCAGCCTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGTGTC13410.1089132632963927No Hit
GTCTGGGGCTGAGGTGAAGACGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCCTCTGGATACACCTTCACGAG13280.10785743002058874No Hit
ATCCAGCCTGGTTCCAGCAGAAACCTGGACAAGCACCCAGGGCACTCATT13220.10737012235483306No Hit
CCTGAGAACCGGGCAGGTGTCCACGAGTGTTTTTTGGTTGAACTGTAAAT13120.10655794291190696No Hit
CTGTATATCCTGCCCATCCACTCAAGAGCCTGTCCGGGGGCCTGGCGCAC13040.10590819935756607No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12980.10542089169181038No Hit
GCCCTGACTCTGTCAGGTGTGCAGCCTGAGGACGAGGCTGACTATTACTG12930.10501480197034734No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12900.10477114813746949No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCCCCTGAGAAC12880.10460871224888427No Hit
CTGTAAATGAGTGCCCTGGGTGCTTGTCCAGGTTTCTGCTGGAACCAGGCTGGATAGTAACCAAAGGTGACCGGT12800.10395896869454338No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12770.10371531486166553No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12710.10322800719590987No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)