FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791922
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCAGGACAGGCCCCTATTCTTGTCATCTACGGTAAAAACAGGCGGCCC41350.5221473832018809No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38040.48035033753324197No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCAG35320.44600352054874093No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCAGATGGTTATCA34600.43691171605284357No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG33380.4215061584347954No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC31530.39814527188283694No Hit
CTACTGTCCCGGGCGTTACAGTAATAATCAGCCTCATCTTCCGCCTGAGC29060.3669553314594114No Hit
GGACAGTAGTGATAACCATCTGCTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC28350.3579898020259571No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26760.3379120670975172No Hit
ATGTAAGCTGGTTCCAGCAGAACCCAGGACAGGCCCCTATTCTTGTCATC25130.3173292319193052No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24620.31088920373471124No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC24220.3058382012369905No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC23630.29838797255285243No Hit
GTCCTGGGTTCTGCTGGAACCAGCTTACATCATGCCTTCTGACACTGTCTCCTTGGCATGTGATCCTGACTGT23490.2966201216786502No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22860.28866479274474No Hit
GTGTCAGAAGGCATGATGTAAGCTGGTTCCAGCAGAACCCAGGACAGGCC21770.274900810938451No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21050.26580900644255373No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20300.2563383767593273No Hit
CCCCTATTCTTGTCATCTACGGTAAAAACAGGCGGCCCTCAGGGATCCCC19470.24585754657655678No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA18790.23727084233043155No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18460.23310376526981194No Hit
GATTATTACTGTAACGCCCGGGACAGTAGTGATAACCATCTGCTTTTCGG18450.23297749020736888No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCAGATGGTTATCAC18400.23234611489515383No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18100.2285578630218633No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17280.21820330790153575No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCCGTGTCTGTGGCCTTGGGA17030.2150464313404603No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16860.21289975527892896No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16800.21214210490427088No Hit
GTAATAATCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG16440.20759620265632223No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16340.20633345203189205No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15940.2012824495341713No Hit
GAATAGGGGCCTGTCCTGGGTTCTGCTGGAACCAGCTTACATCATGCCTT15670.1978730228482098No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15620.1972416475359947No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15280.1929482954129321No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCC15190.19181181985094492No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14970.18903376847719852No Hit
ACTCAGGACCCTGCCGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGTGTCAGAAGG14880.18789729291521134No Hit
TATCACTACTGTCCCGGGCGTTACAGTAATAATCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG14880.18789729291521134No Hit
GTAGTGATAACCATCTGCTTTTCGGCGGAGGGACCAAGCTGACCGTCCTA14830.18726591760299627No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14060.17754273779488383No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13600.171734084922505No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13560.17122898467273293No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13290.16781955798677142No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12800.16163207992706352No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12700.16036932930263334No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAG12280.15506577668002655No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGATTATTACTGTAACGCCCGG12270.15493950161758355No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11510.14534259687191414No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGTGTCAGAAGGCATGATGTAAGCTGGTT11500.14521632180947114No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11260.1421857203108387No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11240.14193317018595264No Hit
GCCCTCAGGGATCCCCGACCGATTCTCTGGCTCCAGCTCCGGAAACACAG11150.1407966946239655No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC11130.14054414449907945No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10930.1380186432502191No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10650.13448294150181458No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10380.13107351481585308No Hit
GGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCCGTGTCTGTGG10350.13069468962852404No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG10140.12804291331722065No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10130.12791663825477761No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9990.12614878738057536No Hit
GTTATCACTACTGTCCCGGGCGTTACAGTAATAATCAGCCTCATCTTCCG9910.12513858688103122No Hit
AGCTTACATCATGCCTTCTGACACTGTCTCCTTGGCATGTGATCCTGACTGTCTGTCCCAAGGCCACAGACACGG9730.12286563575705689No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9700.12248681056972782No Hit
GTCAGGATCACATGCCAAGGAGACAGTGTCAGAAGGCATGATGTAAGCTGGTTCCAGCAGAACCCAGGACAGGCC9580.12097150982041162No Hit
TTCTGACACTGTCTCCTTGGCATGTGATCCTGACTGTCTGTCCCAAGGCC9380.11844600857155124No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9360.1181934584466652No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9200.11617305744757692No Hit
ACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACT9100.11491030682314672No Hit
GATGTAAGCTGGTTCCAGCAGAACCCAGGACAGGCCCCTATTCTTGTCAT9080.11465775669826068No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA8960.11314245594894445No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8930.11276363076161541No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC8920.1126373556991724No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8900.11238480557428637No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT8890.11225853051184333No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCAGATGGTTAT8700.109859304325426No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8660.10935420407565391No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8530.10771262826389469No Hit
GCTTGGTCCCTCCGCCGAAAAGCAGATGGTTATCACTACTGTCCCGGGCGTTACAGTAATAATCAGCCTCATCTT8460.10682870282679355No Hit
GCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGTGTCAGAAG8400.10607105245213544No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGT8390.10594477738969242No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC8380.1058185023272494No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8370.1056922272648064No Hit
GTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCTGTAGCACTGTC8280.10455575170281921No Hit
TCCCTGAGGGCCGCCTGTTTTTACCGTAGATGACAAGAATAGGGGCCTGTCCTGGGTTCTGCTGGAACCAGCTTA8230.10392437639060413No Hit
TTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCCGTGT8210.10367182626571808No Hit
CCTCAGGGATCCCCGACCGATTCTCTGGCTCCAGCTCCGGAAACACAGCTTCCTTGACCATCACTGGGGCTCAGG8140.10278790082861697No Hit
CTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAG8050.10165142526662979No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT8020.10127260007930074No Hit
CTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAACATCCGAATTATTGTTCCT7960.10051494970464263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC501.0913936E-955.33731541
CGTACTA606.1390892E-945.53077729
ACCGTAC606.199116E-945.48184627
GACCGTA651.2607416E-841.96997526
GTATCAA14650.039.7732731
AATGGTA6350.038.35550770
GGTATCA6200.037.9219171
TATCAAC16250.035.0183752
TCGTATG709.606047E-734.55702640
GTACTAG807.710878E-834.1718730
TCAACGC16700.034.0747644
ATCAACG16850.033.771433
CCGTCTT957.965355E-933.01521347
TAGTCAC1154.7293724E-1132.9616738
CAACGCA17300.032.8929825
ATGCCGT958.278221E-932.8856644
TATACAC1155.4569682E-1132.5932543
AACGCAG17550.032.4244236
ATCTCCG959.929863E-932.27728310
TCTCGTA652.3390927E-531.80916438
Produced by FastQC (version 0.11.5)