FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791922
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCAGGACAGGCCCCTATTCTTGTCATCTACGGTAAAAACAGGCGGCCC39460.4982813964001505No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35700.4508019729215756No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCAG32140.4058480506918611No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC31650.3996605726321532No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG29670.3746581102684355No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCAGATGGTTATCA28960.3656925808349812No Hit
GGACAGTAGTGATAACCATCTGCTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC27470.3468775965309715No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27380.34574112096898435No Hit
CTACTGTCCCGGGCGTTACAGTAATAATCAGCCTCATCTTCCGCCTGAGC25020.31594020623243196No Hit
ACTCAGGACCCTGCCGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGTGTCAGAAGG24200.3055856511121045No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24200.3055856511121045No Hit
ATGTAAGCTGGTTCCAGCAGAACCCAGGACAGGCCCCTATTCTTGTCATC24100.3043229004876743No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23620.2982616974904094No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22560.2848765408714495No Hit
GTGTCAGAAGGCATGATGTAAGCTGGTTCCAGCAGAACCCAGGACAGGCC22130.2794467131863997No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC20410.2577274024462005No Hit
GTCCTGGGTTCTGCTGGAACCAGCTTACATCATGCCTTCTGACACTGTCTCCTTGGCATGTGATCCTGACTGT20410.2577274024462005No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCCGTGTCTGTGGCCTTGGGA19710.24888814807518925No Hit
CCCCTATTCTTGTCATCTACGGTAAAAACAGGCGGCCCTCAGGGATCCCC19590.247372847325873No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19120.24143791939105114No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18950.23929124332951984No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA18640.23537671639378624No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18490.233482590457141No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18180.2295680635214074No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17800.22476961114857275No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17450.22034998396306707No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCC17070.21555153159023235No Hit
GATTATTACTGTAACGCCCGGGACAGTAGTGATAACCATCTGCTTTTCGG17020.21492015627801725No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16990.21454133109068824No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15510.1958526218491215No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15370.19408477097491925No Hit
GTAGTGATAACCATCTGCTTTTCGGCGGAGGGACCAAGCTGACCGTCCTA15300.1932008455378181No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14980.18916004353964153No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCAGATGGTTATCAC14930.18852866822742642No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14210.17943686373152912No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13970.17640626223289668No Hit
GTAATAATCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG13850.17489096148358046No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13800.17425958617136536No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13660.1724917352971631No Hit
GAATAGGGGCCTGTCCTGGGTTCTGCTGGAACCAGCTTACATCATGCCTT13640.17223918517227707No Hit
TATCACTACTGTCCCGGGCGTTACAGTAATAATCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG13390.1690823086112016No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13150.16605170711256917No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13080.16516778167546806No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAG11950.15089869961940697No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11740.14824692330810357No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11630.1468578976212304No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11610.14660534749634432No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11510.14534259687191414No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGTGTCAGAAGGCATGATGTAAGCTGGTT11510.14534259687191414No Hit
GCCCTCAGGGATCCCCGACCGATTCTCTGGCTCCAGCTCCGGAAACACAG11450.14458494649725603No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11260.1421857203108387No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGATTATTACTGTAACGCCCGG11200.1414280699361806No Hit
GGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCCGTGTCTGTGG10880.137387267938004No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10500.1325888155651693No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10460.13208371531539723No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10300.13006331431630894No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10030.12665388763034743No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9910.12513858688103122No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG9890.12488603675614518No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC9800.123749561194158No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC9740.12299191081949991No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9610.12135033500774066No Hit
GACTCAGGACCCTGCCGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCA9550.12059268463308255No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9330.11781463325933614No Hit
GTCAGGATCACATGCCAAGGAGACAGTGTCAGAAGGCATGATGTAAGCTGGTTCCAGCAGAACCCAGGACAGGCC9260.11693070782223501No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9200.11617305744757692No Hit
ACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACT9110.11503658188558974No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9010.11377383126115956No Hit
AGCTTACATCATGCCTTCTGACACTGTCTCCTTGGCATGTGATCCTGACTGTCTGTCCCAAGGCCACAGACACGG8850.11175343026207127No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8710.109985579387869No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA8710.109985579387869No Hit
GTTATCACTACTGTCCCGGGCGTTACAGTAATAATCAGCCTCATCTTCCG8680.10960675420053996No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT8660.10935420407565391No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8600.10859655370099582No Hit
CCTCAGGGATCCCCGACCGATTCTCTGGCTCCAGCTCCGGAAACACAGCTTCCTTGACCATCACTGGGGCTCAGG8480.10708125295167958No Hit
TTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCCGTGT8390.10594477738969242No Hit
CTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAG8270.10442947664037619No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC8260.10430320157793317No Hit
TTCTGACACTGTCTCCTTGGCATGTGATCCTGACTGTCTGTCCCAAGGCC8260.10430320157793317No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8240.10405065145304715No Hit
GATGTAAGCTGGTTCCAGCAGAACCCAGGACAGGCCCCTATTCTTGTCAT8240.10405065145304715No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8200.10354555120327506No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT8130.10266162576617394No Hit
CTTCTGAGCTGACTCAGGACCCTGCCGTGTCTGTGGCCTTGGGACAGACA8090.10215652551640186No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCAGATGGTTAT8060.1017777003290728No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC8060.1017777003290728No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT8020.10127260007930074No Hit
GCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGTGTCAGAAG7960.10051494970464263No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAT200.00777230551.0382271
CGACGAG305.782448E-446.82238451
GTAGTTT902.4563742E-644.17314570
CCAACCG350.001315092339.62460343
AATGGTA6300.037.86269470
GGTATCA7100.037.380111
GTATCAA13950.033.415711
GTGGTAT2050.031.5358181
GCACGCG550.00428730131.1402669
CGAAATT450.00434857431.05096448
CGAACTA553.4873048E-430.99882929
CGCACCT553.521089E-430.94789715
CCACGAT703.7728468E-430.58418769
AGCACGA450.004933534730.26016619
ACGGGAT450.004938110730.25443317
GTCTATG450.00494574530.2448791
TATCAAC15550.029.7511062
GTGGCCG2951.8189894E-1229.19919670
GTAATAG704.230948E-529.1647031
CTAGTCA500.006201413528.87652264
Produced by FastQC (version 0.11.5)