FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1042313
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGTCTGAATGGTGTGGTGTTCGG79880.7663724812028632No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG71640.6873175332169895No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC57630.5529049335468329No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT50860.4879532347768856No Hit
GCCTTGGGCTGACCTAGGACGGCCAGCTTGGTCCCTCCGCCGAACACCACACCATTCAGACTGTCATCCCATG47730.4579238673987564No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG42840.411008977149858No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGCCAGCTTGGTCCCTC38890.3731124911614841No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC38160.3661088367889492No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC37450.35929706335812756No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT36920.3542122184027255No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG33810.32437473196630956No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32510.311902470754946No Hit
GTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT32190.3088323756875334No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30910.29655199541788313No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG30610.29367378129218386No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT29410.2821609247893867No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG28880.27707607983398463No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28790.2762126155962748No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT28760.2759247941837049No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC28360.27208717534943916No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG27850.2671942113357504No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG27060.2596149141380756No Hit
GACCTAGGACGGCCAGCTTGGTCCCTCCGCCGAACACCACACCATTCAGA26320.252515319294684No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25670.24627918868900225No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT25520.24484008162615262No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25420.2438806769175862No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25400.2436887959758729No Hit
CCCCTGAGGGCCGCTGATTATTATTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGATGCTGGTACCAGTTTA24500.23505415359877502No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG24140.23160029664793588No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23980.2300652491142296No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23890.22920178487651982No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23790.2282423801679534No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGTCTGAATGGTGTGGTGTTCGGCGGAGGGAC23510.2255560469839674No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC23470.22517228510054083No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC22440.2152904166023066No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA21980.21087715494290102No Hit
GGATGACAGTCTGAATGGTGTGGTGTTCGGCGGAGGGACCAAGCTGGCCGTCCTAGGTCAGCCCAAGGCTGCCCC21840.20953398835090806No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT21180.2032019172743696No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC20840.19993994126524375No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19660.18861896570415987No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19540.18746768005388018No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19400.18612451346188716No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC19390.18602857299103054No Hit
GTCTGAATGGTGTGGTGTTCGGCGGAGGGACCAAGCTGGCCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA19290.1850691682824641No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT19270.18487728734075082No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19230.18449352545732425No Hit
GTGTGGTGTTCGGCGGAGGGACCAAGCTGGCCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC19080.1830544183944746No Hit
GAAGTAATACTGTAAACTGGTACCAGCATCTCCCAGGAACGGCCCCCAAA18460.17710610920136274No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC18210.17470759742994668No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC18110.17374819272138023No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG17380.1667445383488453No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG17170.1647297884608558No Hit
GTGCAGCATGGGATGACAGTCTGAATGGTGTGGTGTTCGGCGGAGGGACC17030.1633866218688628No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16940.16252315763115302No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC16900.16213939574772643No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC16860.1617556338642999No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16660.15983682444716704No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGCCAGCTTGGTCCCTCCGCCGAACACCACAC16660.15983682444716704No Hit
GCATGGGATGACAGTCTGAATGGTGTGGTGTTCGGCGGAGGGACCAAGCTGGCCGTCCTAGGTCAGCCCAAGG16510.15839771738431738No Hit
CCATTCAGACTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTC16510.15839771738431738No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA16500.15830177691346073No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC16490.15820583644260408No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16450.15782207455917752No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT16210.1555195032586181No Hit
ACACCACACCATTCAGACTGTCATCCCATGCTGCACAGTAATAATCAGCC15850.15206564630777894No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15820.15177782489520902No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC15570.14937931312379296No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC15540.14909149171122302No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15420.14794020606094332No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15370.1474605037066601No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15200.14582951570209715No Hit
GTTTGGGGGCCGTTCCTGGGAGATGCTGGTACCAGTTTACAGTATTACTT14980.14371882534325103No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG14680.14084061121755173No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14290.13709893285414268No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14240.13661923049985947No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14220.13642734955814614No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG14220.13642734955814614No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTAAACTGGTACCAGCATCTCCCAGGAACGGCCCC14210.13633140908728952No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC14190.13613952814557623No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGCCAGCTTGGTC14120.13546794484957972No Hit
TGGGATGACAGTCTGAATGGTGTGGTGTTCGGCGGAGGGACCAAGCTGGCCGTCCTAGGTCAGCCCAAGGCTGCC13730.13172626648617064No Hit
ACCTAGGACGGCCAGCTTGGTCCCTCCGCCGAACACCACACCATTCAGAC13550.1299993380107511No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13530.1298074570690378No Hit
ATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATG13450.12903993330218466No Hit
GTTCCTGGGAGATGCTGGTACCAGTTTACAGTATTACTTCCGATGTTGGA13370.1282724095353315No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC13320.1277927071810483No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGACCTCAGCTTCAGGGAGA13240.12702518341419514No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC13210.12673736200162522No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13070.12539419540963223No Hit
GAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACGCTGAGGGTGG12960.12433885023020916No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT12860.12337944552164273No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC12590.12078905280851336No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA12480.1197337076290903No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG12410.11906212433309381No Hit
CTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAG12190.11695143397424765No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG12160.11666361256167773No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG12160.11666361256167773No Hit
TTACTGTGCAGCATGGGATGACAGTCTGAATGGTGTGGTGTTCGGCGGAG11880.11397727937769173No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC11860.11378539843597846No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC11850.1136894579651218No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC11840.11359351749426515No Hit
GATTATTATTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGATGCTGG11840.11359351749426515No Hit
GTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCC11770.11292193419826867No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG11750.11273005325655536No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA11730.11253817231484209No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC11720.11244223184398545No Hit
GTTTACAGTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGA11710.1123462913731288No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG11690.11215441043141551No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA11550.11081124383942251No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG11510.11042748195599596No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG11380.10918025583485959No Hit
GAATGGTGTGGTGTTCGGCGGAGGGACCAAGCTGGCCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT11240.10783708924286659No Hit
GGTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGA11090.10639798218001695No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC11070.10620610123830367No Hit
GTCAGGGACCCCTGAGGGCCGCTGATTATTATTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGATGCTGGTA10940.1049588751171673No Hit
GGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGTCTGAATGGTGTGGTGTTCGGCGGAGGGACCAAG10930.10486293464631066No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC10900.10457511323374072No Hit
GTGTTCGGCGGAGGGACCAAGCTGGCCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC10840.10399947040860087No Hit
GCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAG10820.10380758946688759No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATAATAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG10780.10342382758346103No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT10760.10323194664174772No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10680.10246442287489459No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG10650.10217660146232466No Hit
GACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC10650.10217660146232466No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG10520.10092937534118832No Hit
ATAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG10460.10035373251604844No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAG10440.10016185157433516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACCG300.002113768670.9043570
GTATAGG150.002494053268.012381
TAGTGAG200.00779931350.994615
GGTATCA8350.048.8711741
GCCGTCT1100.047.42805546
TCGTATG1100.046.99674640
TGATAGC306.4721657E-445.76542337
GTGGTAT1950.045.3415831
CGTATGC1150.045.0344441
TATTGAT5400.044.6434870
ACGAGAC1300.044.46536622
CACGAGA1300.044.46323421
TGGTATC2100.042.10292
TCTCGTA1000.041.24881438
ATCTCGT1000.041.1888837
CATCTCG1000.041.13508636
GTATCAA23450.040.749421
TGCCGTC1300.040.0685145
CCGTCTT1400.039.796147
CTAGCGC1300.039.30014428
Produced by FastQC (version 0.11.5)