FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1042313
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGTCTGAATGGTGTGGTGTTCGG71800.6888525807506959No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC55500.5324696132543679No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT47090.45178367726393126No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC44830.43010113085032997No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG43900.4211786670606622No Hit
GCCTTGGGCTGACCTAGGACGGCCAGCTTGGTCCCTCCGCCGAACACCACACCATTCAGACTGTCATCCCATG36670.3518137066313094No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33820.3244706724371662No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGCCAGCTTGGTCCCTC33450.3209208750154704No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32480.31161464934237604No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG31890.30595416156183414No Hit
GTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT31770.30480287591155436No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT31680.30393941167384464No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG31530.302500304610995No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT31490.30211654272756844No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG30430.29194685281676425No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28310.2716074729951559No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG27910.2677698541608902No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27510.2639322353266245No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC27490.2637403543849112No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26100.2504046289358379No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24790.23783642725361767No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC24470.23476633218620513No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23610.22651545169253381No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23570.22613168980910725No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23450.2249804041588275No Hit
CCCCTGAGGGCCGCTGATTATTATTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGATGCTGGTACCAGTTTA23170.22229407097484155No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT23100.22162248767884501No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22770.21845645214057582No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT22240.21337160718517376No Hit
GGATGACAGTCTGAATGGTGTGGTGTTCGGCGGAGGGACCAAGCTGGCCGTCCTAGGTCAGCCCAAGGCTGCCCC22190.21289190483089054No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22150.21250814294746395No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGTCTGAATGGTGTGGTGTTCGGCGGAGGGAC21930.2103974525886178No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG21800.20915022646748144No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21460.2058882504583556No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA21420.205504488574929No Hit
GTGTGGTGTTCGGCGGAGGGACCAAGCTGGCCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC20530.1969657866686878No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20420.19591044148926473No Hit
GAAGTAATACTGTAAACTGGTACCAGCATCTCCCAGGAACGGCCCCCAAA20380.19552667960583817No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC20350.19523885819326825No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20070.19255252500928222No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19600.18804332287902004No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT19340.18554887063674733No Hit
GACCTAGGACGGCCAGCTTGGTCCCTCCGCCGAACACCACACCATTCAGA19060.18286253745276132No Hit
GTCTGAATGGTGTGGTGTTCGGCGGAGGGACCAAGCTGGCCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA19020.18247877556933473No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC18280.17537918072594316No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC18220.1748035379008033No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18190.17451571648823339No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17380.1667445383488453No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG17230.16530543128599567No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG17010.16319474092714953No Hit
GTTTGGGGGCCGTTCCTGGGAGATGCTGGTACCAGTTTACAGTATTACTT16970.16281097904372296No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG16940.16252315763115302No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA16690.16012464585973696No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16500.15830177691346073No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC16380.15715049126318104No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT16360.15695861032146774No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTAAACTGGTACCAGCATCTCCCAGGAACGGCCCC16230.15571138420033137No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT16010.15360069384148523No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15670.1503387178323594No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC15630.14995495594893282No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15410.14784426559008665No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG15340.14717268229409017No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15170.14554169428952723No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15100.14487011099353073No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC14980.14371882534325103No Hit
GTGCAGCATGGGATGACAGTCTGAATGGTGTGGTGTTCGGCGGAGGGACC14670.14074467074669508No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC14510.1392096232129888No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGCCAGCTTGGTCCCTCCGCCGAACACCACAC14350.1376745756792825No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14300.1371948733249993No Hit
GCATGGGATGACAGTCTGAATGGTGTGGTGTTCGGCGGAGGGACCAAGCTGGCCGTCCTAGGTCAGCCCAAGG14160.1358517067330063No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14020.1345085401410133No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13820.13258973072388044No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13710.13153438554445737No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT13060.12529825493877558No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG12940.12414696928849586No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC12910.12385914787592595No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12820.12299568363821616No Hit
TGGGATGACAGTCTGAATGGTGTGGTGTTCGGCGGAGGGACCAAGCTGGCCGTCCTAGGTCAGCCCAAGGCTGCC12690.1217484575170798No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC12690.1217484575170798No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATAATAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG12660.12146063610450988No Hit
ATAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG12650.12136469563365324No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12600.12088499327937002No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGACCTCAGCTTCAGGGAGA12460.11954182668737702No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC12380.11877430292052388No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC12240.11743113632853086No Hit
ACACCACACCATTCAGACTGTCATCCCATGCTGCACAGTAATAATCAGCC12220.11723925538681759No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG12190.11695143397424765No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12070.11580014832396794No Hit
GTTCCTGGGAGATGCTGGTACCAGTTTACAGTATTACTTCCGATGTTGGA12070.11580014832396794No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC11960.11474480314454488No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG11950.11464886267368822No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG11900.11416916031940501No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT11880.11397727937769173No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC11850.1136894579651218No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGCCAGCTTGGTC11790.11311381513998194No Hit
CCATTCAGACTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTC11710.1123462913731288No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC11450.10985183913085608No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG11370.10908431536400295No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA11300.10841273206800643No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG11230.10774114877200995No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACT11230.10774114877200995No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA11150.1069736250051568No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC11030.10582233935487709No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11030.10582233935487709No Hit
GTTTACAGTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGA11010.10563045841316379No Hit
GAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACGCTGAGGGTGG10900.10457511323374072No Hit
GATTATTATTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGATGCTGG10860.10419135135031415No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG10810.10371164899603093No Hit
GGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGTCTGAATGGTGTGGTGTTCGGCGGAGGGACCAAG10750.10313600617089108No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC10710.1027522442874645No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC10650.10217660146232466No Hit
GTGTTCGGCGGAGGGACCAAGCTGGCCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC10640.10208066099146801No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC10620.10188878004975474No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAATT100.0068474635146.6961470
GGTCGTA200.00781568250.96755619
GTCGTAA200.007818649550.96266620
TATTGAT6200.048.50436470
GGTATCA10050.044.3051031
GTGGTAT3150.041.0035631
TGGTATC3150.040.989782
GTATCAA26000.040.0033721
TAAGTCA1553.126661E-837.85706370
ATATTCA800.001922619936.67403470
GGATGGG4900.035.92558370
TATCAAC29000.035.3843732
ATCAACG30200.034.079433
ATAACCT400.002804830433.9604387
CATCGAG502.0424859E-433.95880513
CTATTGA5600.033.8908569
TCAACGC30200.033.849614
CAACGCA30750.033.2425735
AACGCAG31000.033.0840386
ACGCAGA32350.031.7034077
Produced by FastQC (version 0.11.5)