FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1096566
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45670.4164819992595065No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41910.3821931374855686No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35400.32282598585037287No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31770.2897226432335126No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29070.2651003222788232No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28620.26099660211970827No Hit
GATCTATTCTGCATCCCGTTTACAAAGTGGGGTCCCAATAAGATTCAGTGGCACTGGATCTGGGACGGATTTCA27280.2487766354236772No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26100.23801576922866477No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25660.2340032428508635No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25520.23272652991247222No Hit
GCGTTACAATACGCCTCGCACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT24910.22716370925233864No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24810.22625177143920203No Hit
ATTCTGCATCCCGTTTACAAAGTGGGGTCCCAATAAGATTCAGTGGCACTGGATCTGGGACGGATTTCACTCTCA23670.21585568036944425No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23520.21448777364973928No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23380.21321106071134796No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22500.2051860079557455No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22210.2025413882976492No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22040.2009910940153169No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21780.19862005570116162No Hit
ACTTACTACTGTCAACAGCGTTACAATACGCCTCGCACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAA20770.1894094837884815No Hit
CTCCTGTACAGCCTCTGGATTCACCTTCAGTAGTTACTGGATGCACTGGGTCCGCCAAGCTGCAGGGGAGGGG19550.1782838424682144No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGCGTTACAATACG19490.17773667978033242No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18940.17272102180808088No Hit
CAATAAGATTCAGTGGCACTGGATCTGGGACGGATTTCACTCTCACCATCAGCAGCCTGCAACCTGAAGATTTTG18820.17162669643231687No Hit
GTACTAATGCTCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCAACAGATGCAGACAGGGAAGATGGAGA18560.1692556581181616No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18280.16670223224137898No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18020.16433119392722373No Hit
GAATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATT17470.15931553595497217No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17400.1586771794857765No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17380.15849479192314916No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC16730.15256719613776099No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16560.15101690185542868No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG16340.14901063866652806No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16190.14764273194682306No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15960.1455452749766088No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15930.14527169363266781No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15730.1434478180063945No Hit
CTGCTACTCTGGCTCCGAGATGCCAGATGTGACATCCAGATGACCCAGTC14980.13660828440786965No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTG14790.13487560256291004No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14730.13432843987502804No Hit
CACCATCAGCAGCCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGCGTTACAATACGCCTCGCACGTT14540.1325957580300684No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14450.13177501399824543No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14440.13168382021693176No Hit
ACAATACGCCTCGCACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGA14320.1305894948411678No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14240.12985994459065847No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14060.1282184565270125No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13710.12502667418103425No Hit
ATCTTATTGGGACCCCACTTTGTAAACGGGATGCAGAATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTTTG13690.12484428661840691No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13610.1241147363678976No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGTACTTATTTAAATTGGTATCAGCAAAAA13540.12347637989870194No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGATGCCAGATGTGACAT13530.12338518611738829No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13510.12320279855476096No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13360.12183489183505598No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13360.12183489183505598No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13230.12064937267797834No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13160.1200110162087827No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13010.11864310948907772No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC12970.11827833436382307No Hit
GCCCCTAACCTCCTGATCTATTCTGCATCCCGTTTACAAAGTGGGGTCCCAATAAGATTCAGTGGCACTGGAT12970.11827833436382307No Hit
GTATTATTGTGTAAGAGGAGCCTCAGACGGTGACGACGCTGAGGGGGGGTACTGGGGCCAGGGAACCCTGGTCAC12890.11754878411331375No Hit
TTGTAACGCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGGCTGCTGATGGTGAGAGTGAAATCCGTC12880.11745759033200008No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12870.11736639655068641No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12870.11736639655068641No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12820.11691042764411809No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12810.11681923386280442No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12770.11645445873754977No Hit
ATAATACACAGCCGTGTCGTCGGCTCTGAGACTGTTCATTTGCAGATACA12770.11645445873754977No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12670.11554252092441313No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12670.11554252092441313No Hit
ACTGTCAACAGCGTTACAATACGCCTCGCACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTG12540.11435700176733547No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG12500.11399222664208082No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12490.11390103286076717No Hit
CAATAATACACAGCCGTGTCGTCGGCTCTGAGACTGTTCATTTGCAGATA12480.1138098390794535No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12420.1132626763915715No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12300.11216835101580754No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12280.11198596345318021No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12240.11162118832792554No Hit
GTCTCCAACAGATGCAGACAGGGAAGATGGAGACTGGGTCATCTGGATGT12060.10997970026427957No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12020.10961492513902492No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11980.10925015001377025No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGGCTGCTGATGGTGAGAGTGAAATCCGTCCCAGATCCAGTGCCA11910.10861179354457459No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11900.10852059976326095No Hit
ATGCAGAATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAATAAGTACTAATGCT11800.1076086619501243No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11750.10715269304355597No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11730.10697030548092865No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11710.10678791791830132No Hit
TTACAATACGCCTCGCACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT11610.10587598010516468No Hit
GGTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAATAAGTACTA11580.10560239876122367No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11500.10487284851071435No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11370.10368732935363671No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11210.10222822885261808No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11160.10177225994604976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1950.051.33020840
CGTATGC2050.050.56605541
CGAGACG2500.050.43428423
ATGCCGT1950.047.99652544
AATCTCG2050.046.8911936
ACGAGAC2700.046.683522
CGCTCAT2900.044.73457728
CTTATAC2950.043.8600541
GCCGTCT2200.042.6433446
GTATCAA16350.042.2752151
ATCTCCG3100.041.7377910
CATGAAT2800.041.57110632
GAGACGC3050.041.34335324
CACGAGA3050.041.3244921
CCCACGA3050.041.31318319
TACACAT3150.041.0752875
GTATGCC2650.040.4641142
TATACAC3300.040.240023
ACACATC3200.039.3694466
ACGGGTT350.001437790338.9134255
Produced by FastQC (version 0.11.5)