FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1096566
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42380.3864792452073108No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38510.35118725183892263No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34330.3130682512498108No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33850.3086909497467549No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30130.2747668630980716No Hit
GATCTATTCTGCATCCCGTTTACAAAGTGGGGTCCCAATAAGATTCAGTGGCACTGGATCTGGGACGGATTTCA26360.24038680754282005No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26050.23755980032209642No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24630.22461028337555605No Hit
GCGTTACAATACGCCTCGCACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT24290.22150969481089144No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24270.22132730724826413No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23760.21667642440126722No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23410.21348464205528894No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23140.21102240995982002No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23050.21020166592799705No Hit
ATTCTGCATCCCGTTTACAAAGTGGGGTCCCAATAAGATTCAGTGGCACTGGATCTGGGACGGATTTCACTCTCA22840.2082865965204101No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22720.2071922711446461No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22570.20582436442494112No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22120.20172064426582623No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21610.19706976141882931No Hit
CTCCTGTACAGCCTCTGGATTCACCTTCAGTAGTTACTGGATGCACTGGGTCCGCCAAGCTGCAGGGGAGGGG21020.1916893283213231No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19860.181110849688938No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19750.1801077180944877No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGCGTTACAATACG18630.16989401458735726No Hit
ACTTACTACTGTCAACAGCGTTACAATACGCCTCGCACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAA18390.16770536383582932No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18070.16478716283379202No Hit
GTACTAATGCTCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCAACAGATGCAGACAGGGAAGATGGAGA17960.16378403123934174No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17350.15822121057920818No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16570.15110809563674232No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16280.14846347597864606No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16230.14800750707207774No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16080.14663960035237278No Hit
CAATAAGATTCAGTGGCACTGGATCTGGGACGGATTTCACTCTCACCATCAGCAGCCTGCAACCTGAAGATTTTG16040.14627482522711813No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15970.14563646875792247No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG15740.14353901178770817No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15560.14189752372406222No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC15510.14144155481749388No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGTACTTATTTAAATTGGTATCAGCAAAAA15330.13980006675384793No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15240.13897932272202493No Hit
GAATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATT15120.13788499734626097No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTG14900.13587873415736035No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14890.13578754037604668No Hit
CTGCTACTCTGGCTCCGAGATGCCAGATGTGACATCCAGATGACCCAGTC14890.13578754037604668No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14670.13378127718714605No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14360.13095426996642245No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14200.12949516946540382No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14140.12894800677752183No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13850.12630338711942557No Hit
CACCATCAGCAGCCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGCGTTACAATACGCCTCGCACGTT13660.12457070527446593No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13640.1243883177118386No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13630.12429712393052493No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGATGCCAGATGTGACAT13330.12156131049111497No Hit
ACAATACGCCTCGCACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGA13300.12128772914717399No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13300.12128772914717399No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13280.12110534158454667No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13210.120466985115351No Hit
ATCTTATTGGGACCCCACTTTGTAAACGGGATGCAGAATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTTTG13080.11928146595827337No Hit
GCCCCTAACCTCCTGATCTATTCTGCATCCCGTTTACAAAGTGGGGTCCCAATAAGATTCAGTGGCACTGGAT12660.11545132714309947No Hit
GTCTCCAACAGATGCAGACAGGGAAGATGGAGACTGGGTCATCTGGATGT12650.1153601333617858No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12650.1153601333617858No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12640.11526893958047214No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12240.11162118832792554No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12160.11089163807741624No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12080.1101620878269069No Hit
GTATTATTGTGTAAGAGGAGCCTCAGACGGTGACGACGCTGAGGGGGGGTACTGGGGCCAGGGAACCCTGGTCAC11880.10833821220063361No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11880.10833821220063361No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11840.10797343707537896No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC11810.10769985573143795No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGGCTGCTGATGGTGAGAGTGAAATCCGTCCCAGATCCAGTGCCA11780.10742627438749697No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11750.10715269304355597No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11700.10669672413698766No Hit
TTGTAACGCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGGCTGCTGATGGTGAGAGTGAAATCCGTC11700.10669672413698766No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11700.10669672413698766No Hit
ACTGTCAACAGCGTTACAATACGCCTCGCACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTG11490.10478165472940071No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11400.10396091069757771No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11360.10359613557232306No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11320.1032313604470684No Hit
GGTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAATAAGTACTA11270.10277539154050007No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11240.10250181019655909No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11020.10049554700765845No Hit
GAGTGAAATCCGTCCCAGATCCAGTGCCACTGAATCTTATTGGGACCCCA10970.10003957810109014No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTA200.007799341350.99472812
TAGTACG200.007837418550.9319464
CGCAATT305.342665E-447.5813960
GTGGTAT2100.040.4313961
GTATCAA17700.039.7187041
TATCAAC19350.035.6232762
ATAGGAC400.002806619533.9561773
ATCAACG20300.033.6216353
TCAACGC20450.033.3734974
CAACGCA20600.033.298355
AACGCAG21000.032.6715476
AATGGTA5250.032.34954570
CGGTACC553.1389392E-431.56153545
AGAGTAC22200.030.78060211
ACGCAGA22350.030.7051017
CCGCGCA450.00465689430.62032338
CGCAGAG22550.030.2904078
TATTAGA803.125484E-629.7116572
GCAGAGT23250.029.529999
GAGTTTA4050.029.3501971
Produced by FastQC (version 0.11.5)