FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245408
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGATAGTTCACCGATCACCTTCGGCCAAGGGA52540.4218697808268455No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36880.2961278552891904No Hit
GTATGATAGTTCACCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT35510.2851274441789357No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33650.2701925794599039No Hit
GTAATAAAAGGACGTGTCCTCACTTCTCAGACTGTTCATTTGCAGATACA32070.25750597394588765No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCGTACCCACTGCCA29730.23871695058968628No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26230.21061371052699196No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26210.21045312058377655No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGT25460.20443099771319923No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA25280.20298568822426064No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24400.19591973072278324No Hit
GTGTATTACTGTCAGCAGTATGATAGTTCACCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA24260.19479560112027544No Hit
CTATCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC23550.18909465813612888No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23430.1881311184768365No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21540.17295536884298157No Hit
CTCCAGGCCCTTCCCTGGAGCTTGCCGGACCCAGTGCATGGCATAATCAT20960.1682982604897351No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20750.16661206608597343No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTC20350.1634002672216655No Hit
CTCCTGTGCAGCCTCTGGATTTAACTTTGATGATTATGCCATGCACTGGGTCCGGCAAGCTCCAGGGAAGGGC20200.16219584264755005No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20160.16187466276111925No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20050.16099141807343456No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20030.16083082813021918No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTCGCAGCTACTTAGCCTGGCACCAGC19650.15777961920912664No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19550.15697666949304967No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19160.15384516560034944No Hit
CTTTTATTACTGTGTAAAAGATACGTCGGTGGGACCTAGGTCCCGTGGTT19110.15344369074231096No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19010.15264074102623398No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19000.15256044605462626No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18860.1514363164521185No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18580.14918805724710296No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18340.14726097792851822No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18230.14637773324083353No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18200.14613684832601043No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18080.14517330866671807No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCGCAG17940.1440491790642103No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17400.1397132505973946No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16970.13626056681826357No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16930.13593938693183277No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16910.1357787969886174No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT16870.1354576171021866No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16770.13465466738610962No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC16680.13393201264164034No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCGTACCCACTGCCA16630.13353053778360185No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGCCCCTTGGCCCCAGATATCAAAACCACGGGAC16430.13192463835144788No Hit
GGCCTGGAGTGGGTCTCAGATATTAGTTGGAATAGTGGTAGCATAGCCTA16400.13168375343662478No Hit
GTACGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT16200.13007785400447083No Hit
GTCTGAGAAGTGAGGACACGTCCTTTTATTACTGTGTAAAAGATACGTCG15870.12742811994141678No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15370.12341337136103189No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15320.12301189650299339No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15270.1226104216449549No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15220.12220894678691642No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCGCAGCTACTTAGCCTGGCA15190.12196806187209333No Hit
CTTCTCAGACTGTTCATTTGCAGATACAGGGAGTTCTTGGCGTTGTCTCT15050.12084393226958555No Hit
CTCCAGGGAAGGGCCTGGAGTGGGTCTCAGATATTAGTTGGAATAGTGGT14890.11955921272386238No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC14810.1189168529510008No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC14730.11827449317813922No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14690.11795331329170843No Hit
CCATTGCCCCTTGGCCCCAGATATCAAAACCACGGGACCTAGGTCCCACC14570.11698977363241606No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14510.11650800380276986No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGATAGTTCA14220.11417944962614662No Hit
GTCAGAGTGTTCGCAGCTACTTAGCCTGGCACCAGCAGAAACCTGGCCAGGCTCCCAGGCTTCTCATCTCTGGTG14130.11345679488167734No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14130.11345679488167734No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13830.11104794573344638No Hit
AGCATAGCCTATGCGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCCCTGTATCTG13810.11088735579023098No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTTCTCATCTCTGGTGCATCCAGCAGGGCCACTGCCATCCCAGACAGGTT13680.10984352115933091No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13420.10775585189753077No Hit
ACCTAGGTCCCACCGACGTATCTTTTACACAGTAATAAAAGGACGTGTCC13320.10695290218145379No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC13280.10663172229502299No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT13250.1063908373801999No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGA13020.10454405303322285No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12950.10398198823196896No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12930.10382139828875357No Hit
GACCTAGGTCCCGTGGTTTTGATATCTGGGGCCAAGGGGCAATGGTCACCGTCTCTTCAGCATCCCCGACCAGCC12790.10269726868624579No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC12680.10181402399856111No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA12640.10149284411213032No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC12610.10125195919730723No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12560.10085048433926874No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG12480.10020812456640715No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGC252.670426E-454.7614941
TACCGCT200.00659202453.21082361
CGTATGC1950.046.0805641
GACCGTA2050.043.424226
CGTACTA2100.042.4294929
ACCGTAC2100.042.4022127
GCCGTCT2150.042.0257746
TCGTATG2150.041.74152440
GGTATCA9500.041.4313961
ACGAGAC2150.041.38451822
GTACTAG2200.040.51391230
ATCTCGT2050.040.31059637
GTATGCC2450.039.54252242
ATGCCGT2300.039.19864744
GTATCAA24400.037.311881
CACGAGA2450.036.3141121
CCGTCTT2500.036.17461447
CTAGATC2200.035.90566633
TACTAGA2400.035.729531
AGTATAG1101.164067E-535.22269470
Produced by FastQC (version 0.11.5)