FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245408
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGATAGTTCACCGATCACCTTCGGCCAAGGGA48660.3907153318430587No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37070.2976534597497366No Hit
GTATGATAGTTCACCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT36040.28938307767414373No Hit
GTAATAAAAGGACGTGTCCTCACTTCTCAGACTGTTCATTTGCAGATACA30030.24112579973791723No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCGTACCCACTGCCA29200.2344613170944783No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28120.22578946016084692No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27390.21992792723348492No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27160.21808114288650787No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25440.20427040776998381No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGT25100.20154037873532207No Hit
GTGTATTACTGTCAGCAGTATGATAGTTCACCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA24430.1961606156376063No Hit
CTATCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC21510.1727144839281585No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21000.16861944037616589No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20920.1679770806033043No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20900.16781649066008889No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20850.16741501580205043No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20550.16500616665381945No Hit
CTCCTGTGCAGCCTCTGGATTTAACTTTGATGATTATGCCATGCACTGGGTCCGGCAAGCTCCAGGGAAGGGC20450.16420321693774248No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20140.16171407281790384No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20040.16091112310182687No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19460.1562540147485804No Hit
CTCCAGGCCCTTCCCTGGAGCTTGCCGGACCCAGTGCATGGCATAATCAT18980.15239985611141088No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18430.1479836326729875No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTCGCAGCTACTTAGCCTGGCACCAGC18130.14557478352475653No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18050.14493242375189497No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTC17630.14156003494437164No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCGCAGCTACTTAGCCTGGCA17140.13762558133559444No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17110.13738469642077134No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17050.13690292659112516No Hit
CTTTTATTACTGTGTAAAAGATACGTCGGTGGGACCTAGGTCCCGTGGTT17050.13690292659112516No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16610.13336994784038644No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16590.13320935789717106No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCGCAG16400.13168375343662478No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC16210.13015814897607852No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16180.12991726406125542No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16110.12935519926000155No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCGTACCCACTGCCA16020.12863254451553227No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15960.12815077468588607No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15910.12774929982784758No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15750.12646458028212443No Hit
GTACGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT15720.1262236953673013No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC15650.12566163056604743No Hit
GTCTGAGAAGTGAGGACACGTCCTTTTATTACTGTGTAAAAGATACGTCG15630.12550104062283202No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGCCCCTTGGCCCCAGATATCAAAACCACGGGAC15610.12534045067961663No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15500.12445720599193197No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14890.11955921272386238No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14880.11947891775225469No Hit
CTTCTCAGACTGTTCATTTGCAGATACAGGGAGTTCTTGGCGTTGTCTCT14870.11939862278064699No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14840.1191577378658239No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14700.1180336082633161No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14610.11731095351884682No Hit
GGCCTGGAGTGGGTCTCAGATATTAGTTGGAATAGTGGTAGCATAGCCTA14590.11715036357563142No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC14500.11642770883116214No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14470.11618682391633907No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGATAGTTCA14060.11289473008042346No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14060.11289473008042346No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13920.11177060047791566No Hit
CTCCAGGGAAGGGCCTGGAGTGGGTCTCAGATATTAGTTGGAATAGTGGT13730.11024499601736942No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13660.10968293121611551No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG13600.10920116138646933No Hit
CCATTGCCCCTTGGCCCCAGATATCAAAACCACGGGACCTAGGTCCCACC13450.10799673681235386No Hit
GTCAGAGTGTTCGCAGCTACTTAGCCTGGCACCAGCAGAAACCTGGCCAGGCTCCCAGGCTTCTCATCTCTGGTG13260.10647113235180759No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC13090.10510611783447674No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13030.10462434800483054No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12980.10422287314679206No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTTCTCATCTCTGGTGCATCCAGCAGGGCCACTGCCATCCCAGACAGGTT12860.10325933348749967No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12690.10189431897016882No Hit
ACCTAGGTCCCACCGACGTATCTTTTACACAGTAATAAAAGGACGTGTCC12570.10093077931087643No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)