FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004687983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857257
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23030.2686475584334686No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20560.23983472867529806No Hit
GAGTTATGGTGTCTCCTTCACTTTCGGCCCTGGGACCACAGTGGATATCAAACGAACTGTGGCTGCACCAT20380.237735008288063No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17800.20763901607102653No Hit
GTCTGAGACAGACTTCGCTCTCACCATCACCAATCTACAACCTGAAGACT16430.19165781090151496No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16260.18967474164690404No Hit
GTCCAGGGAAGGGCCTGGAGTGGGTCTCAGGGATCAATTGGAATAGTGCT15780.18407548728094375No Hit
ACTTACTACTGTCAACAGAGTTATGGTGTCTCCTTCACTTTCGGCCCTGGGACCACAGTGGATATCAAACGAA14750.17206042062065402No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14580.17007735136604307No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14510.1692607934376739No Hit
CCATAACTCTGTTGACAGTAGTAAGTAGCAGAGTCTTCAGGTTGTAGATTGGTGATGGTGAGAGCGAAGT13820.161211865286606No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13480.15724572677738416No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13050.15222972807454474No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13020.1518797746766722No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12520.1460472180454636No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12030.1403313125468792No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11880.1385815455575166No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11600.13531531384403978No Hit
TCCCTGGACCTTGCCGGACCCAGTGCATGGCATAATCGTCAAAGGTGTAT11520.1343821047830464No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11320.13204908213056296No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10990.12819959475396525No Hit
CCTGAAGACTCTGCTACTTACTACTGTCAACAGAGTTATGGTGTCTCCTT10910.12726638569297188No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10690.1247000607752401No Hit
GTAGTAAGTAGCAGAGTCTTCAGGTTGTAGATTGGTGATGGTGAGAGCGAAGTCTGTCTCAGACCCACTGCCA10620.12388350284687089No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC10600.12365020058162256No Hit
GTAGGAGACAGAGTCACCATCACCTGCCGGGCAAGTCAGCCCATTAAAAGTTATATAAATTGGTTTCAGCACAAA10570.12330024718375003No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10270.11980071320502486No Hit
CTCCTGATCTATGATATATTCACTCTGCAAACTGGGGTCCCATCGAGGTTCAGTGGCAGTGGGTCTGAGACAGA10180.11875085301140732No Hit
GTTATGGTGTCTCCTTCACTTTCGGCCCTGGGACCACAGTGGATATCAAA10110.11793429508303811No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG10000.1166511326241722No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9970.1163011792262997No Hit
GTCTCAGACCCACTGCCACTGAACCTCGATGGGACCCCAGTTTGCAGAGT9940.11595122582842717No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9880.11525131903268215No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9830.11466806336956128No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9780.11408480770644042No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9690.11303494751282288No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9490.11070192486033943No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9410.10976871579934605No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTGTGGTCCCAGGGCCGAAAGTG9370.10930211126884938No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTGTGGTCC9350.10906880900360101No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9300.10848555334048017No Hit
CACCATCACCAATCTACAACCTGAAGACTCTGCTACTTACTACTGTCAACAGAGTTATGGTGTCTCCTTCACTTT9230.10766899541211096No Hit
ATGATATATTCACTCTGCAAACTGGGGTCCCATCGAGGTTCAGTGGCAGTGGGTCTGAGACAGACTTCGCTCTCA9040.10545262389225168No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9030.10533597275962751No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9010.10510267049437917No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8790.10253634557664737No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8790.10253634557664737No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8760.10218639217877486No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8680.10125318311778149No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8620.10055327632203645No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)