FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857257
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21040.24543398304125835No Hit
GAGTTATGGTGTCTCCTTCACTTTCGGCCCTGGGACCACAGTGGATATCAAACGAACTGTGGCTGCACCAT19350.22571994162777326No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18000.20997203872350997No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17350.2023897151029388No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15230.17765967498661428No Hit
GTCTGAGACAGACTTCGCTCTCACCATCACCAATCTACAACCTGAAGACT14540.16961074683554642No Hit
GTCCAGGGAAGGGCCTGGAGTGGGTCTCAGGGATCAATTGGAATAGTGCT14450.16856088664192884No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13700.15981205169511595No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13670.1594620982972434No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13570.15829558697100168No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13190.15386284393128316No Hit
CCATAACTCTGTTGACAGTAGTAAGTAGCAGAGTCTTCAGGTTGTAGATTGGTGATGGTGAGAGCGAAGT12750.14873019409581958No Hit
ACTTACTACTGTCAACAGAGTTATGGTGTCTCCTTCACTTTCGGCCCTGGGACCACAGTGGATATCAAACGAA12540.14628052031071195No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11710.13659847630290567No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11530.13449875591567056No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11280.13158247760006625No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11000.12831624588658944No Hit
GTAGGAGACAGAGTCACCATCACCTGCCGGGCAAGTCAGCCCATTAAAAGTTATATAAATTGGTTTCAGCACAAA10170.11863420187878315No Hit
TCCCTGGACCTTGCCGGACCCAGTGCATGGCATAATCGTCAAAGGTGTAT10050.11723438828729307No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10020.11688443488942057No Hit
CTCCTGATCTATGATATATTCACTCTGCAAACTGGGGTCCCATCGAGGTTCAGTGGCAGTGGGTCTGAGACAGA9850.11490136563480963No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9760.11385150544119207No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9750.11373485430856792No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9690.11303494751282288No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9660.11268499411495037No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9650.11256834298232618No Hit
GTTATGGTGTCTCCTTCACTTTCGGCCCTGGGACCACAGTGGATATCAAA9510.11093522712558779No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9450.11023532032984275No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9390.10953541353409771No Hit
GTAGTAAGTAGCAGAGTCTTCAGGTTGTAGATTGGTGATGGTGAGAGCGAAGTCTGTCTCAGACCCACTGCCA9390.10953541353409771No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTGTGGTCC9360.10918546013622518No Hit
CCTGAAGACTCTGCTACTTACTACTGTCAACAGAGTTATGGTGTCTCCTT9360.10918546013622518No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9330.10883550673835268No Hit
GTCTCAGACCCACTGCCACTGAACCTCGATGGGACCCCAGTTTGCAGAGT9270.10813559994260764No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9260.10801894880998347No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9220.10755234427948678No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC9160.10685243748374176No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9100.10615253068799672No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9060.10568592615750003No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTGTGGTCCCAGGGCCGAAAGTG8960.1045194148312583No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8930.10416946143338578No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCG200.00770905951.1441731
GCTTATA200.00782407550.952941
GTGGTAT2550.042.6272961
TGGTATC2700.041.4905472
GGTATCA7400.039.9360921
TTTAGCG350.001452373638.8326326
GTATAGA350.001454460638.8212851
GTATCAA18000.035.1009141
CCGTATT400.002818934233.92502614
TCCGTAT400.002823775633.9131513
ATCAACG20450.030.3565653
AACGCAG20800.030.3297216
ACTCGCA450.00499656530.18199221
TCAACGC20600.030.1320064
ATCAATC802.9371422E-629.9482536
CAACGCA20800.029.8405325
CGCAGAG21450.029.4106398
ACGCAGA21500.029.3422417
TATCAAC21650.029.0076682
AGAGTAC22200.028.4153811
Produced by FastQC (version 0.11.5)