FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences983412
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGACTGGCAGTAATATTCACCCTCATCCTCAGCCTGGAGGCCAG47910.487181364473893No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC42280.4299317071583426No Hit
GCCTTGGGCTGACTGAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCATCCACTCACGGAACTGTCATAGGA38320.3896637421548649No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34030.34604011340109736No Hit
GTTCACTGGTACGTGCAGATTCCAGGGAAAGCCCCCAAAGTCCTCATCCG33280.3384136048777115No Hit
GTCCTGGGCCCAGTCTGTATTGACGCAGCCACCTTCAGTGTCTGGGGCCCCAGGACAGACGATTCTCATCTCCTG31380.3190931166184671No Hit
GTCCTATGACAGTTCCGTGAGTGGATGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTCAGTCAGCCCAAGG29520.30017937548047No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28620.2910275652524069No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTGAGGACGGTCAGCCTGGTCCCTC27480.2794352722968603No Hit
CTCCTGCACTGGGGCCACGTCCAACCTCGGGGCAGGTTGGCCTGTTCACTGGTACGTGCAGATTCCAGGGAAAGC27190.27648635566781776No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA25940.2637755081288412No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT25060.2548270714614017No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23480.23876056017213537No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT23210.23601501710371645No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22560.22940537638344866No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT21790.22157549429943907No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG21750.22116874717819185No Hit
ACCCTCAGGAGCCCCTGACCGAATCTCTGGCTCCAAGTCTGGCACCTCAG21670.22035525293569733No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21460.21821983054914929No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19390.19717066702460415No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG19180.19503524463805608No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18940.19259476191057257No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18580.18893403781934734No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18310.1861884947509284No Hit
GTCCTCATCCGTGATGGCGACGAGCGACCCTCAGGAGCCCCTGACCGAAT18050.1835446384628213No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17850.18151090285658503No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17590.1788670465684779No Hit
CCTGAGGGTCGCTCGTCGCCATCACGGATGAGGACTTTGGGGGCTTTCCC16800.17083379092384474No Hit
GACTGAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCATCCACTCACG16640.16920680243885575No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16310.16585113868856594No Hit
CCTTCAGTGTCTGGGGCCCCAGGACAGACGATTCTCATCTCCTGCACTGG16120.1639190898626415No Hit
GCACAGGGTCCTGGGCCCAGTCTGTATTGACGCAGCCACCTTCAGTGTCT15690.15954655830923356No Hit
GGATGAGGGTGAATATTACTGCCAGTCCTATGACAGTTCCGTGAGTGGATGGGTGTTCGGCGGAGGGACCAGG15590.15852969050611543No Hit
GATGAGGACTTTGGGGGCTTTCCCTGGAATCTGCACGTACCAGTGAACAG15520.15781788304393274No Hit
CCCTCATCCTCAGCCTGGAGGCCAGTGATTTTCAGGGAGGCTGAGGTGCC15500.15761450948330913No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15420.15680101524081463No Hit
GTATTGACGCAGCCACCTTCAGTGTCTGGGGCCCCAGGACAGACGATTCT15240.154970653195202No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14920.15171667622522403No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTATTGACGCAGCCACCTTCAGTGTCTGGGGCCCCAGGACAGACGATTCT14880.15130992910397678No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14850.15100486876304134No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTGAGGACGGTCAGCCTGGTCCCTCCGCCGAACAC14770.15019137452054684No Hit
TCCTCAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14740.1498863141796114No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14480.14724245789150428No Hit
GCCTGGAGGCCAGTGATTTTCAGGGAGGCTGAGGTGCCAGACTTGGAGCC14250.1449036619443326No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14170.14409016770183808No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14130.14368342058059086No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC14020.14256486599716092No Hit
CCCCAGGACAGACGATTCTCATCTCCTGCACTGGGGCCACGTCCAACCTC13640.13870076834531203No Hit
CCCATCCACTCACGGAACTGTCATAGGACTGGCAGTAATATTCACCCTCA13620.13849739478468842No Hit
GCCTGGTCCCTCCGCCGAACACCCATCCACTCACGGAACTGTCATAGGACTGGCAGTAATATTCACCCTCATCCT13530.1375822137618821No Hit
CCAGTGAACAGGCCAACCTGCCCCGAGGTTGGACGTGGCCCCAGTGCAGGAGATGAGAATCGTCTGTCCTGGGGC13530.1375822137618821No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13460.13687040629969943No Hit
GTAATAAACGGCCGAGTCTGCGGCTGTCATAGAAGTCAGTTTGAGGAAGA13160.13381980289034504No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12750.12965064489756073No Hit
GTGCAGGAGATGAGAATCGTCTGTCCTGGGGCCCCAGACACTGAAGGTGG12700.12914221099600168No Hit
GTCTGGCACCTCAGCCTCCCTGAAAATCACTGGCCTCCAGGCTGAGGATG12610.12822702997319538No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12350.12558317368508823No Hit
CCTCCAGGCTGAGGATGAGGGTGAATATTACTGCCAGTCCTATGACAGTT12180.12385449841978743No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTGAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCATCCACTCA11930.12131232891199213No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11620.11816003872232594No Hit
GGACCAGGCTGACCGTCCTCAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG11530.11724485769951964No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11510.11704148413889601No Hit
CGTCCAACCTCGGGGCAGGTTGGCCTGTTCACTGGTACGTGCAGATTCCA11380.11571955599484245No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT11320.11510943531297156No Hit
CCTCAGCTGTGGGCCCAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC11260.11449931463110069No Hit
GGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTATTGACGCAGCCACCTT11260.11449931463110069No Hit
GGACAGACGATTCTCATCTCCTGCACTGGGGCCACGTCCAACCTCGGGGCAGGTTGGCCTGTTCACTGGTACGTG11170.11358413360829439No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCA11100.1128723261461117No Hit
GTGCCAGACTTGGAGCCAGAGATTCGGTCAGGGGCTCCTGAGGGTCGCTC11070.11256726580517627No Hit
GTAATATTCACCCTCATCCTCAGCCTGGAGGCCAGTGATTTTCAGGGAGG11050.11236389224455264No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10970.11155039800205815No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT10940.1112453376611227No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10910.11094027732018727No Hit
CCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTC10900.11083859053987546No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10840.11022846985800458No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG10810.10992340951706914No Hit
ATTCTCATCTCCTGCACTGGGGCCACGTCCAACCTCGGGGCAGGTTGGCC10620.10799136069114472No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA10540.10717786644865021No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC10450.10626268542584391No Hit
TCCTCAGCCTGGAGGCCAGTGATTTTCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGATTCGGTCAGGGGCT10400.10575425152428485No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10120.1029070216755541No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT10060.10229690099368323No Hit
GCCTCAGCTGTGGGCCCAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT10040.10209352743305959No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9910.10077159928900603No Hit
GTCTGGGGCCCCAGGACAGACGATTCTCATCTCCTGCACTGGGGCCACGT9890.10056822572838242No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC9880.1004665389480706No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9880.1004665389480706No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAA350.005701191755.1956370
TACGTAT503.6087225E-451.51592370
TCTCGTA1450.049.90757438
CGTATGC1500.048.41284641
CACGAGA1700.044.20978521
ATCTCGT1650.043.79078737
AGGGGGG2550.042.92993570
CCGGAGT4650.042.9299370
GGTATCA9650.042.490021
ATGCCGT1800.042.40925644
TTAACGG651.1819793E-842.2800135
AATCTCG1650.041.65644536
TCGTATG1800.040.31084440
GCCGTCT1900.040.26036546
TCTCCGA1900.039.55612611
TATAGCC350.001391692239.1718831
CCCACGA1950.038.53794519
GGCAATC2000.037.73951333
ACGAGAC2000.037.5783222
AACCGTG753.3971446E-837.50959454
Produced by FastQC (version 0.11.5)