FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences983412
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGACTGGCAGTAATATTCACCCTCATCCTCAGCCTGGAGGCCAG43730.4446762903035553No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC42300.4301350807189662No Hit
GTCCTGGGCCCAGTCTGTATTGACGCAGCCACCTTCAGTGTCTGGGGCCCCAGGACAGACGATTCTCATCTCCTG40030.4070521815881848No Hit
GCCTTGGGCTGACTGAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCATCCACTCACGGAACTGTCATAGGA37310.3793933773433718No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35770.36373361317535274No Hit
GTTCACTGGTACGTGCAGATTCCAGGGAAAGCCCCCAAAGTCCTCATCCG32510.33058372279370196No Hit
GTCCTATGACAGTTCCGTGAGTGGATGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTCAGTCAGCCCAAGG29710.3021114243063945No Hit
GTATTGACGCAGCCACCTTCAGTGTCTGGGGCCCCAGGACAGACGATTCT29130.29621359104830935No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25780.2621485196438522No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25720.26153839896198133No Hit
CTCCTGCACTGGGGCCACGTCCAACCTCGGGGCAGGTTGGCCTGTTCACTGGTACGTGCAGATTCCAGGGAAAGC25610.2604198443785514No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT23920.24323477850585512No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTGAGGACGGTCAGCCTGGTCCCTC23550.23947236763431806No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23510.23906562051307081No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22970.23357453437623296No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22610.2299138102850077No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22560.22940537638344866No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22170.22543959195128796No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20930.21283043119262324No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20870.21222031051075235No Hit
ACCCTCAGGAGCCCCTGACCGAATCTCTGGCTCCAAGTCTGGCACCTCAG20650.20998320134389248No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19440.19767910092616323No Hit
GCACAGGGTCCTGGGCCCAGTCTGTATTGACGCAGCCACCTTCAGTGTCT18840.19157789410745446No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18290.18598512119030478No Hit
GTCCTCATCCGTGATGGCGACGAGCGACCCTCAGGAGCCCCTGACCGAAT18140.1844598194856276No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18020.18323957812188585No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTGAGGACGGTCAGCCTGGTCCCTCCGCCGAACAC17780.18079909539440234No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17720.18018897471253145No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17250.1754096960378763No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTATTGACGCAGCCACCTTCAGTGTCTGGGGCCCCAGGACAGACGATTCT17060.17347764721195186No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16900.17185065872696287No Hit
CCTTCAGTGTCTGGGGCCCCAGGACAGACGATTCTCATCTCCTGCACTGG16180.1645292105445124No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15110.15364872505114846No Hit
GGATGAGGGTGAATATTACTGCCAGTCCTATGACAGTTCCGTGAGTGGATGGGTGTTCGGCGGAGGGACCAGG15050.15303860436927758No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14880.15130992910397678No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC14580.1482593256946224No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14460.14703908433088064No Hit
GCCTGGAGGCCAGTGATTTTCAGGGAGGCTGAGGTGCCAGACTTGGAGCC14130.14368342058059086No Hit
CCTGAGGGTCGCTCGTCGCCATCACGGATGAGGACTTTGGGGGCTTTCCC13830.14063281717123646No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13680.13910751546655928No Hit
GATGAGGACTTTGGGGGCTTTCCCTGGAATCTGCACGTACCAGTGAACAG13680.13910751546655928No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13470.1369720930800112No Hit
CCCCAGGACAGACGATTCTCATCTCCTGCACTGGGGCCACGTCCAACCTC13440.1366670327390758No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13410.13636197239814035No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13380.1360569120572049No Hit
CCCTCATCCTCAGCCTGGAGGCCAGTGATTTTCAGGGAGGCTGAGGTGCC13350.1357518517162695No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13310.13534510459502222No Hit
TCCTCAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12970.1318877540644206No Hit
GTAATAAACGGCCGAGTCTGCGGCTGTCATAGAAGTCAGTTTGAGGAAGA12890.1310742598219261No Hit
GCCTGGTCCCTCCGCCGAACACCCATCCACTCACGGAACTGTCATAGGACTGGCAGTAATATTCACCCTCATCCT12790.13005739201880798No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12760.12975233167787256No Hit
GTCTGGCACCTCAGCCTCCCTGAAAATCACTGGCCTCCAGGCTGAGGATG12670.12883715065506623No Hit
GACTGAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCATCCACTCACG12660.12873546387475443No Hit
CCAGTGAACAGGCCAACCTGCCCCGAGGTTGGACGTGGCCCCAGTGCAGGAGATGAGAATCGTCTGTCCTGGGGC12600.12812534319288355No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12460.12670172826851817No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC12410.1261932943669591No Hit
GTGCAGGAGATGAGAATCGTCTGTCCTGGGGCCCCAGACACTGAAGGTGG12350.12558317368508823No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTGAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCATCCACTCA11880.12080389501043305No Hit
GGGTCCTGGGCCCAGTCTGTATTGACGCAGCCACCTTCAGTGTCTGGGGCCCCAGGACAGACGATTCTCATCTC11630.11826172550263776No Hit
GTCTGTATTGACGCAGCCACCTTCAGTGTCTGGGGCCCCAGGACAGACGA11590.11785497838139052No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11500.11693979735858419No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT11320.11510943531297156No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC11320.11510943531297156No Hit
GGACCAGGCTGACCGTCCTCAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG11110.1129740129264235No Hit
CCTCCAGGCTGAGGATGAGGGTGAATATTACTGCCAGTCCTATGACAGTT10860.11043184341862819No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTGAGGACGGTCAGCCTGGTCCCTCCGCCGAACACC10810.10992340951706914No Hit
GGACAGACGATTCTCATCTCCTGCACTGGGGCCACGTCCAACCTCGGGGCAGGTTGGCCTGTTCACTGGTACGTG10810.10992340951706914No Hit
CCTCAGCTGTGGGCCCAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC10800.10982172273675735No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA10700.1088048549336392No Hit
CCCATCCACTCACGGAACTGTCATAGGACTGGCAGTAATATTCACCCTCA10660.10839810781239195No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10650.10829642103208015No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT10490.10666943254709114No Hit
GTGCCAGACTTGGAGCCAGAGATTCGGTCAGGGGCTCCTGAGGGTCGCTC10400.10575425152428485No Hit
GTCTGGGGCCCCAGGACAGACGATTCTCATCTCCTGCACTGGGGCCACGT10240.10412726303929583No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG10220.10392388947867222No Hit
ATTCTCATCTCCTGCACTGGGGCCACGTCCAACCTCGGGGCAGGTTGGCC10180.10351714235742496No Hit
GGGTGAATATTACTGCCAGTCCTATGACAGTTCCGTGAGTGGATGGGTGTTCGGCGGAGGGACCAGGCTGACCGT10060.10229690099368323No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10060.10229690099368323No Hit
GTCTCTGGTGACTCCCTTAAAAGAAACACTTATTACTGGAGTTGGGTGCG10040.10209352743305959No Hit
GTTTATTACTGTGCGAGAGGACTCGTCAAGAGAGGGTGGTTCGCCTCTTT9990.10158509353150054No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9980.10148340675118872No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC9970.1013817199708769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9050.046.3929441
TTGTGAG4500.044.37278470
GTGGTAT1850.038.7474371
GTATCAA24150.037.0322071
AATGGTA4050.036.15560570
ATAGCGG1101.8189894E-1135.8070463
TATCAAC26400.033.219422
ATCAACG27400.032.0005233
TCAACGC27500.031.8874024
GGTGCCA21750.031.5199870
TGGTATC2200.031.022032
AACGCAG28250.030.9200526
CAACGCA28250.030.918485
ATGGCAA3700.030.58124470
TGACTAG450.004880358530.32802410
GTACGAC450.004881572430.326483
CGTGCAA703.261889E-530.29880357
CTTGTGA4800.029.52599569
ACGCAGA30000.029.1163837
TAGCGGC1252.7193892E-928.71459664
Produced by FastQC (version 0.11.5)