FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004687991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1988465
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCCCTGATCTCAGACTGCTCACCTCCAAGTAGA98240.4940494300880327No Hit
GTGTACTCCCAAACTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGAGAAGT79250.3985486292190207No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT68000.3419723253866676No Hit
GCTCAGCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGTTACAGGTGTCCAGTCCCAGGTCCA65660.33020445418953814No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC62370.31365902844656557No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA56250.28288151916176546No Hit
CTTCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCT54940.276293522893287No Hit
GGAGTACACCGTTCACTTTCGGCGGAGGGACCAAGGTCGAGATCAAACGA53470.2689008858591929No Hit
CCATAGAAGAGGAGCCTGGGAGCCTGGCCACGTTGCTGCTGGTACCAGGCTAAGTAGTTGCTGTCAACACTCTGA50810.2555237331308321No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC47090.23681583533026732No Hit
GTCTGGGACAGACTTCTCTCTCACCATCAGCAGACTGGAGCCTGAAGATT46170.23218915092797712No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45510.2288700077698124No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44270.2226340418362908No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT43300.2177559071947457No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39410.19819307858071428No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39300.19763988805435345No Hit
CAGTAATACACGGCCGTGTCCCCTGATCTCAGACTGCTCACCTCCAAGTA38960.19593002642742016No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38250.19235943303000053No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCATGCAGGGCCAGTCAGAGTGTTGACAG37760.18989522068530248No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA36870.18541940642656524No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35090.17646777790909068No Hit
GTGCAGCCACAGTTCGTTTGATCTCGACCTTGGTCCCTCCGCCGAAAGTG34300.17249486412886322No Hit
GTTTGGGAGTACACCGTTCACTTTCGGCGGAGGGACCAAGGTCGAGATCAAACGAACTGTGGCTGCACCAT33990.17093587264548282No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCATGC33970.17083529254978086No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33520.16857224039648674No Hit
GTGTATTACTGTGCGACAGATGGCCGGCGTGAGACCCGCCCGTACTGGGGCCAGGGAAGCCTGGTCACCGTCTC33000.16595715790823576No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32650.16419700623345143No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG32370.16278888489362398No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32320.1625374346543691No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32180.16183337398445535No Hit
GTATTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGC32060.16122989341024357No Hit
CTCCCAAACTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGAGAAGTCTGTC31030.15605001848159258No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG31020.1559997284337416No Hit
ATCTCAGACTGCTCACCTCCAAGTAGACTGTAGACGTGGACTTGTCCGCGGTAATCGTGACTCTGCCCTCGAAGT31010.15594943838589062No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30150.1516244942707063No Hit
CTCTCATGCAGGGCCAGTCAGAGTGTTGACAGCAACTACTTAGCCTGGTA29730.14951231226096512No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAAGGATCATCCCTGTCCTTGGT29690.1493111520695612No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA29380.14775216058618082No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29310.14740013025122395No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCGACCTTGGTCCCTCCGCCGAAAGTGAACGGTGTACT29190.14679664967701217No Hit
GTCCCAGGTCCACCTGGTGCAATCTGGGGCTGAGGTGAGGAAGCCTGGGTCCTCGGTGATGGTCTCCTGCAAGGC29070.1461931691028004No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAACGGTGTACTCCCAAACTGCTGACAGTAATAAACTGCAAAATCTTCAGG28970.1456902686242906No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCATGCAGGGCCAGTCAGA28850.14508678805007885No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACAC28640.14403069704520824No Hit
GTTTATTACTGTCAGCAGTTTGGGAGTACACCGTTCACTTTCGGCGGAGGGACCAAGGTCGAGATCAAACGAA28560.1436283766624004No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28360.14262257570538078No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT27770.13965546288217293No Hit
TCCCTGTCCTTGGTGCTACGACCTACGCACAGGACTTCGAGGGCAGAGTCACGATTACCGCGGACAAGTCCACGT27610.13885082211655725No Hit
GACCAAGGTCGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT27060.13608486948475332No Hit
GTAATAAACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGAGAAGTCTGTCCCAGACCCACTGCCA26910.13533051876698862No Hit
CCTCCAAGTAGACTGTAGACGTGGACTTGTCCGCGGTAATCGTGACTCTG26360.1325645661351847No Hit
CAGTTACAGGTGTCCAGTCCCAGGTCCACCTGGTGCAATCTGGGGCTGAG26180.13165934527386702No Hit
CTCATGCAGGGCCAGTCAGAGTGTTGACAGCAACTACTTAGCCTGGTACC26160.13155876517816506No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC25600.12874252249851015No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25100.12622802010596115No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA25080.12612744001025916No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23960.12049495465094935No Hit
GCATGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAG23940.1203943745552474No Hit
ATCTGGGGCTGAGGTGAGGAAGCCTGGGTCCTCGGTGATGGTCTCCTGCAAGGCCTCTGGAGGCACCTTCAGCAG23640.11888567311971797No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23590.11863422288046307No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23540.11838277264120817No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23400.11767871197129443No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG23260.11697465130138071No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCACCGAGGAAACTGTATTGACGCAGTCTC22790.11461101905238463No Hit
CATAACAACCAGATTCCTCCAGATTCCTCCTCTTAAGAAGCCCCTGGGAGCACAGCTCAGCACCATGGACTGGAC22650.1139069583824709No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22460.11295144747330227No Hit
GTCCAGGGGCCTGTCGCACCCAGCTGATGGTCTCGCTGCTGAAGGTGCCTCCAGAGGCCTTGCAGGAGACCATCA22450.11290115742545129No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22260.11194564651628267No Hit
CTGCTACTCTGGCTCCCAGACACCACCGAGGAAACTGTATTGACGCAGTC22180.11154332613347483No Hit
GGACAGGGATGATCCTTCCCATCCACTCAAGCCCTTGTCCAGGGGCCTGTCGCACCCAGCTGATGGTCTCGCTG21950.11038665503290226No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21950.11038665503290226No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21870.10998433465009441No Hit
GGGCCAGTCAGAGTGTTGACAGCAACTACTTAGCCTGGTACCAGCAGCAA21440.10782186259250225No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG20630.10374836871657282No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20280.10198821704178851No Hit
GACTTCTCTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTTTGGGAGTACA20000.10058009570196105No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)