FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1988465
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCCCTGATCTCAGACTGCTCACCTCCAAGTAGA95160.4785600953499307No Hit
GTGTACTCCCAAACTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGAGAAGT80640.405538945870307No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT69750.3507730837605892No Hit
GCTCAGCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGTTACAGGTGTCCAGTCCCAGGTCCA59150.29746563303854984No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56330.2832838395445733No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54760.2753883020319694No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA53250.2677945048064713No Hit
GGAGTACACCGTTCACTTTCGGCGGAGGGACCAAGGTCGAGATCAAACGA51350.25823939571478505No Hit
CTTCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCT51230.25763591514057327No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49410.2484831264316948No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45680.22972493858327905No Hit
GTCTGGGACAGACTTCTCTCTCACCATCAGCAGACTGGAGCCTGAAGATT43940.22097447025720843No Hit
CCATAGAAGAGGAGCCTGGGAGCCTGGCCACGTTGCTGCTGGTACCAGGCTAAGTAGTTGCTGTCAACACTCTGA43910.22082360011365548No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41050.20644064642827506No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40970.20603832604546723No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40230.20231686250449468No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT39300.19763988805435345No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCATGCAGGGCCAGTCAGAGTGTTGACAG37400.1880847789626672No Hit
CAGTAATACACGGCCGTGTCCCCTGATCTCAGACTGCTCACCTCCAAGTA35680.1794348907322985No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34080.17138848307614163No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34030.17113703283688675No Hit
GTTTGGGAGTACACCGTTCACTTTCGGCGGAGGGACCAAGGTCGAGATCAAACGAACTGTGGCTGCACCAT33930.17063413235837696No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCATGC33480.16837108020508282No Hit
GTGTATTACTGTGCGACAGATGGCCGGCGTGAGACCCGCCCGTACTGGGGCCAGGGAAGCCTGGTCACCGTCTC33340.16766701953516908No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32500.16344265551568674No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32330.16258772470222005No Hit
GTGCAGCCACAGTTCGTTTGATCTCGACCTTGGTCCCTCCGCCGAAAGTG32160.1617327938887534No Hit
CTCCCAAACTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGAGAAGTCTGTC31570.15876568106554553No Hit
GTATTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGC30830.15504421752457298No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30730.15454131704606316No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30720.1544910269982122No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30210.15192623455781218No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29760.14966318240451806No Hit
ATCTCAGACTGCTCACCTCCAAGTAGACTGTAGACGTGGACTTGTCCGCGGTAATCGTGACTCTGCCCTCGAAGT29700.14936144211741217No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29550.14860709139964745No Hit
CTCTCATGCAGGGCCAGTCAGAGTGTTGACAGCAACTACTTAGCCTGGTA29260.147148680011969No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29140.14654519943775726No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCATGCAGGGCCAGTCAGA29070.1461931691028004No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAACGGTGTACTCCCAAACTGCTGACAGTAATAAACTGCAAAATCTTCAGG28880.14523765819363177No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG28290.1422705453704239No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA28130.14146590460480823No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT27710.13935372259506706No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27490.13824734154234547No Hit
GTCCCAGGTCCACCTGGTGCAATCTGGGGCTGAGGTGAGGAAGCCTGGGTCCTCGGTGATGGTCTCCTGCAAGGC27420.13789531120738863No Hit
GTTTATTACTGTCAGCAGTTTGGGAGTACACCGTTCACTTTCGGCGGAGGGACCAAGGTCGAGATCAAACGAA27240.136990090346071No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAAGGATCATCCCTGTCCTTGGT27210.136839220202518No Hit
TCCCTGTCCTTGGTGCTACGACCTACGCACAGGACTTCGAGGGCAGAGTCACGATTACCGCGGACAAGTCCACGT26610.13382181733145918No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCGACCTTGGTCCCTCCGCCGAAAGTGAACGGTGTACT26360.1325645661351847No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACAC26200.131759925369569No Hit
CCTCCAAGTAGACTGTAGACGTGGACTTGTCCGCGGTAATCGTGACTCTG25540.12844078221140426No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC25070.12607714996240818No Hit
CTCATGCAGGGCCAGTCAGAGTGTTGACAGCAACTACTTAGCCTGGTACC24930.12537308929249447No Hit
GTAATAAACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGAGAAGTCTGTCCCAGACCCACTGCCA24570.12356264756985917No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC24450.12295916699564739No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24420.12280829685209446No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24250.12195336603862779No Hit
GACCAAGGTCGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT24150.12145046556011799No Hit
GGGCCAGTCAGAGTGTTGACAGCAACTACTTAGCCTGGTACCAGCAGCAA23610.11873480297616504No Hit
GTCCAGGGGCCTGTCGCACCCAGCTGATGGTCTCGCTGCTGAAGGTGCCTCCAGAGGCCTTGCAGGAGACCATCA23250.11692436125352974No Hit
GCATGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAG23170.11652204087072189No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22840.11486246929163953No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22670.11400753847817285No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22640.11385666833461992No Hit
CAGTTACAGGTGTCCAGTCCCAGGTCCACCTGGTGCAATCTGGGGCTGAG22360.11244854699479247No Hit
CTGCTACTCTGGCTCCCAGACACCACCGAGGAAACTGTATTGACGCAGTC22280.11204622661198463No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22200.11164390622917678No Hit
GGACAGGGATGATCCTTCCCATCCACTCAAGCCCTTGTCCAGGGGCCTGTCGCACCCAGCTGATGGTCTCGCTG21930.1102860749372003No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21920.11023578488934932No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21670.10897853369307481No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCACCGAGGAAACTGTATTGACGCAGTCTC21670.10897853369307481No Hit
ATCTGGGGCTGAGGTGAGGAAGCCTGGGTCCTCGGTGATGGTCTCCTGCAAGGCCTCTGGAGGCACCTTCAGCAG21620.10872708345381991No Hit
GACTTCTCTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTTTGGGAGTACA20500.10309459809451009No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20500.10309459809451009No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20460.10289343790310615No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19900.10007719522345125No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19900.10007719522345125No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)