FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004687993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817118
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAAGAGCCGTGTCCCCGGCTCTCAGGCCGCTCATTTGAAGGTATA44940.5499817651795701No Hit
CCATAGTCCCTTGGCCCCAGAAATCACAAGCCCACGACCCGCTTAGCACA24090.2948166605068056No Hit
GGACTATGGTGACCGTCTCTTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGC22150.2710746795444477No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20580.2518608083532611No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20210.24733269858208973No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19170.23460503868474322No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17870.21869546381306004No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17060.2087825748545498No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16370.200338262038041No Hit
CTTTATTACTGTGCAAGAGAGGTTCCCGATGTGCTAAGCGGGTCGTGGGC16240.1987473045508727No Hit
GTATTACTGTCAGAAGTCTGGTGGCTCACCTCCGGTCACCTTCGGCCAAGGGACACGACTGGAGACTAAACGAA15570.19054775442469754No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14630.17904390797901895No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14620.17892152663385216No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14580.17843200125318498No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14290.17488294224334797No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14050.17194578995934492No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13920.1703548324721766No Hit
CTCCTGTGCAGCCTCTGGCTTCACCCTCACTGGGAACGACATGCACTGGGTCCGCCAACCTGCAGGAAAAGGT13780.1686414936398415No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA13710.16778482422367394No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13170.1611762315846671No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12560.15371096952949267No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12490.15285430011332513No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12300.15052905455515606No Hit
GGACTGAACACACAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC12260.15003952917448887No Hit
CATCTATGGTGCATCGAACAGGGCCACTGACATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA12060.14759190227115299No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12050.1474695209259862No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11630.14232950442898087No Hit
GATCAGGACTGAACACACAGGACTCACCATGGAGTTGGGGCTGAGCTGGG11600.1419623603934805No Hit
GCATAGTATGTGTCACCACCACGACCAATACCTGAGACCCACTCCAGACC11510.14086092828697938No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11410.13963711483531144No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11400.13951473349014462No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11120.13608805582547442No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11100.13584329313514082No Hit
GTATGTGTCACCACCACGACCAATACCTGAGACCCACTCCAGACCTTTTC11090.13572091178997403No Hit
CTCTTGCACAGTAATAAAGAGCCGTGTCCCCGGCTCTCAGGCCGCTCATTTGAAGGTATAAGGAATTCTTGAAAT11040.13510900506414006No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10990.1344970983383061No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10900.13339566623180493No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT10880.13315090354147138No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC10840.1326613781608042No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10810.1322942341253038No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10800.13217185278013702No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT10730.13131518336396947No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTCACCATAGTCCCTTGGCCCCAGAAATCACAAGCCCACGACC10600.12972422587680116No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATATTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT10570.12935708184130076No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10340.1265423109024645No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10200.12482897207012941No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGGACCCTGAAGACTTTGGAGTGTATTACTGTCAGAAGT10150.12421706534429544No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC10100.12360515861846147No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10030.1227484892022939No Hit
CTCCTGCAGGGCCAGCCAGAGTGTTAGCAGCACCTACTTAGCCTGGTACC9900.12115753171512561No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC9870.12079038767962522No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT9790.11981133691829086No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9720.11895466750212333No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9450.11565037118261988No Hit
GTGTATTACTGTCAGAAGTCTGGTGGCTCACCTCCGGTCACCTTCGGCCA9350.11442655773095196No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT9270.11344750696961761No Hit
GTCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGCTTCACCCTCACTGGGAACGACATG9240.11308036293411722No Hit
GCCTTGGGATTCCCAAGTGTTTGTATTCAGTGATCAGGACTGAACACACA9150.11197893082761608No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTCACCATAGTCCCTTGG9120.11161178679211571No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG8990.11002082930494739No Hit
GTGTTTGTATTCAGTGATCAGGACTGAACACACAGGACTCACCATGGAGT8980.1098984479597806No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT8980.1098984479597806No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGCCAGAGTGTTAGCAG8910.10904177854361304No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT8760.10720605836611113No Hit
CAGTAATACACTCCAAAGTCTTCAGGGTCCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA8670.10610462625961No Hit
GACACATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACGATTTCAAGAATTCCTTATACCTT8670.10610462625961No Hit
GTGTTAGCAGCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT8660.10598224491444319No Hit
GTAATACACTCCAAAGTCTTCAGGGTCCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA8650.1058598635692764No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG8650.1058598635692764No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8640.10573748222410961No Hit
GCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGTTGAGGGAACTGGGCAGGGCACAGT8410.10292271128527336No Hit
GAACTGGGCAGGGCACAGTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGG8390.10267794859493977No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGTCAGTGGCCCTGTT8380.10255556724977298No Hit
GCTTAGCACATCGGGAACCTCTCTTGCACAGTAATAAAGAGCCGTGTCCC8350.10218842321427261No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8340.1020660418691058No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA8320.10182127917877223No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)