FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817118
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAAGAGCCGTGTCCCCGGCTCTCAGGCCGCTCATTTGAAGGTATA41330.5058020995743577No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24860.30424002408464873No Hit
GGACTATGGTGACCGTCTCTTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGC21640.2648332309409412No Hit
CTTTATTACTGTGCAAGAGAGGTTCCCGATGTGCTAAGCGGGTCGTGGGC21570.26397656152477367No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19790.2421926820850844No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16820.20584542257054672No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16730.20474399046404557No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16510.20205160087037613No Hit
CTCCTGTGCAGCCTCTGGCTTCACCCTCACTGGGAACGACATGCACTGGGTCCGCCAACCTGCAGGAAAAGGT16360.20021588069287422No Hit
GTATTACTGTCAGAAGTCTGGTGGCTCACCTCCGGTCACCTTCGGCCAAGGGACACGACTGGAGACTAAACGAA16040.1962996776475368No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15730.19250585594736624No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14820.18136915353718802No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14360.17573961165951552No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13930.17047721381734338No Hit
GGACTGAACACACAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC13140.1608090875491667No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13020.15934051140716518No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA13020.15934051140716518No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATATTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT12990.15897336737166481No Hit
GTCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGCTTCACCCTCACTGGGAACGACATG12470.15260953742299155No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12350.15114096128099No Hit
GATCAGGACTGAACACACAGGACTCACCATGGAGTTGGGGCTGAGCTGGG12300.15052905455515606No Hit
GACACATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACGATTTCAAGAATTCCTTATACCTT12160.14881571572282093No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11920.1458785634388179No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11890.14551141940331752No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11820.14465474998714994No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11810.14453236864198316No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11730.14355331788064882No Hit
CTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACGATTTCAAGAATTCCTTATACCTTCAAATGAG11660.14269664846448127No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11540.14122807232247975No Hit
GTATGTGTCACCACCACGACCAATACCTGAGACCCACTCCAGACCTTTTC11280.1380461573481431No Hit
CATCTATGGTGCATCGAACAGGGCCACTGACATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA11200.13706710658680876No Hit
GTGTTTGTATTCAGTGATCAGGACTGAACACACAGGACTCACCATGGAGT11160.13657758120614158No Hit
GGTCTGGAGTGGGTCTCAGGTATTGGTCGTGGTGGTGACACATACTATGC11110.1359656744803076No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11010.1347418610286397No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10910.13351804757697175No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10730.13131518336396947No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG10650.1303361326026351No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10360.12678707359279812No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10330.12641992955729772No Hit
GCCTTGGGATTCCCAAGTGTTTGTATTCAGTGATCAGGACTGAACACACA10240.12531849745079657No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10210.12495135341529619No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10130.12397230265396185No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGGACCCTGAAGACTTTGGAGTGTATTACTGTCAGAAGT10130.12397230265396185No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10120.12384992130879506No Hit
GCATAGTATGTGTCACCACCACGACCAATACCTGAGACCCACTCCAGACC10030.1227484892022939No Hit
CTCCTGCAGGGCCAGCCAGAGTGTTAGCAGCACCTACTTAGCCTGGTACC9970.12201420113129315No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9850.12054562498929164No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC9800.11993371826345767No Hit
CTCTTGCACAGTAATAAAGAGCCGTGTCCCCGGCTCTCAGGCCGCTCATTTGAAGGTATAAGGAATTCTTGAAAT9740.11919943019245689No Hit
GGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATATTAGAAGGTGTCCAGTGTGAGGTGCAG9710.11883228615695651No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC9680.11846514212145613No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9640.11797561674078898No Hit
GTCTGGGGGAGTCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTG9560.11699656597945463No Hit
GTGTTAGCAGCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT9410.11516084580195272No Hit
CTTTATTACTGTGCAAGAGAGGTTCCCGATGTGCTAAGCGGGTCGTGGGCTTGTGATTTCTGGGGCCAAGGGA9340.11430417638578516No Hit
ATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACGATT9260.11332512562445081No Hit
GTGTATTACTGTCAGAAGTCTGGTGGCTCACCTCCGGTCACCTTCGGCCA9230.11295798158895043No Hit
GTGCAAGAGAGGTTCCCGATGTGCTAAGCGGGTCGTGGGCTTGTGATTTC9220.11283560024378364No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGTCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC9190.11246845620828327No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9160.11210131217278287No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9110.11148940544694892No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG9030.11051035468561457No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGTGGAGCCCCAGCCTTGGGATTCCCAAGTGTTTGTATTCAGTGAT8830.1080627277822787No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGCTTCACCCTCACTGGGAACGAC8750.10708367702094435No Hit
CAGTAATACACTCCAAAGTCTTCAGGGTCCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA8600.10524795684344243No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8540.10451366877244168No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT8510.1041465247369413No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8490.10390176204660771No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8450.10341223666594054No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT8370.10243318590460618No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGCCAGAGTGTTAGCAG8200.1003527030367707No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGA200.007913968550.8056952
TGTCGAT200.00791588350.80258615
TACCTTA306.8577914E-445.2298426
GGTATCA7300.044.0887951
GTATCAA18300.035.9151841
ACGGGAT1052.0008883E-1135.48985716
TCGTCTC10000.034.43538365
AATCGTC10050.034.35560263
GAAATCG9900.034.29052761
AACGGCC400.002683606534.2669437
TAACGGC400.002702403834.21819736
TTAACGG400.002716364634.1822635
ATCGTCT10100.033.94832664
CTCTGGA12150.033.94776569
AAATCGT10250.033.5793762
GGGACTA10650.032.5649170
TCGGTTT850.003749826732.00150370
TATCAAC21000.030.9672812
AATGGTA2001.664921E-830.60143770
AACTGTA900.00495995630.22364270
Produced by FastQC (version 0.11.5)