FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525207
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT20950.39889034228408987No Hit
ATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGTCCACCT16130.3071170033910439No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12810.24390383220330272No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT11830.2252445226358369No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11800.2246733192817308No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11050.21039323542907845No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT11030.21001243319300772No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC10640.2025867895896285No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10530.20049237729123948No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG10280.19573234934035533No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10280.19573234934035533No Hit
GTATAATACTTACTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT9910.18868750797304681No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8840.16831458834326274No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8540.16260255480220182No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8380.15955613691363596No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA8130.15479610896275184No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG8030.15289209778239818No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC7920.15079768548400915No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7750.14756086647740796No Hit
GGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGTCCACCTCCGCC6780.12909195802797754No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6500.12376072672298731No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6340.12071430883442147No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG6310.12014310548031537No Hit
GGACAGGCGGGTGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAA6250.11900069877210319No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG6160.11728708870978491No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6070.11557347864746663No Hit
CCTTCGCTGTGACCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAG5940.11309826411300687No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT5750.10948064287033493No Hit
GCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCCCTGC5720.10890943951622883No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT5610.10681502721783982No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5500.10472061491945081No Hit
GGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCAT5430.10338780709320326No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG5270.10034138920463742No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAAT200.007198541452.03520242
CGGTATC307.2449836E-444.7253762
TCGTATG2500.040.0394940
GTATCAA30850.039.57871
CGTATGC2450.039.54851541
ACATGCG2400.039.2918327
GTATGCC2550.038.09112542
GTGGTAT3800.037.9577261
ATGCCGT2500.037.63208844
CGAGACA2600.037.46086523
GCGCAGA2550.037.19029631
ATCTCGT2400.037.08798237
GGTATCA13350.036.936131
ACGAGAC2650.036.74705522
CTCGTAT2550.036.4472239
TGCGCAG2600.036.41558530
CATGCGC2700.034.9661828
CCGTCTT2750.034.4494947
ATGCGCA2800.033.7658629
CTAACGC502.1938854E-433.5440333
Produced by FastQC (version 0.11.5)