FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525207
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23170.44115939048794095No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT18010.34291241358169255No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12690.24161901878687833No Hit
ATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGTCCACCT12320.23457417741956982No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11190.21305885108157352No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT10720.2041099985339114No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9870.18792590350090535No Hit
GTATAATACTTACTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT9410.17916745207127857No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9140.1740266218843237No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC8330.15860413132345913No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8200.15612891678899937No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7970.15174969107418598No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7680.1462280586511604No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7590.1445144485888421No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7540.1435624429986653No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC7420.1412776295822409No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG7390.1407064262281348No Hit
GGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGTCCACCTCCGCC7290.13880241504778118No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA7280.1386120139297458No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7230.13766000833956896No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG6770.12890155690994218No Hit
CTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAGAAGACCATCGACCGCTTGGCGGGTAAACC6730.1281399524378007No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG6120.11652548423764343No Hit
GGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCAT5740.10929024175229958No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA5730.1090998406342642No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5570.10605342274569837No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT5560.10586302162766299No Hit
GGACAGGCGGGTGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAA5510.10491101603748616No Hit
CCTTCGCTGTGACCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAG5450.10376860932927398No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC5330.1014837959128496No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGT100.008566335136.0952870
CGGACTA150.001740356674.46451664
CCTAACG950.052.846842
CTAACGC850.051.179153
TAGACCG200.00817247250.3916825
GGTATCA12950.038.269271
GTATCAA29700.038.220951
GTGGTAT4400.036.529761
CCGAAAC551.3703584E-436.3386168
ACGCCAT1250.034.7951936
AACGCCA1250.034.7951935
GTACACG400.003003888833.485611
CCGTGTG400.003016537533.4569179
TGGTATC5000.032.800272
TATCAAC34900.032.2229422
ATCAACG35150.032.178073
TCAACGC35250.031.9888084
CGCTCAC3300.031.45617918
CAACGCA36200.031.238785
TCGGCGG3400.030.68010127
Produced by FastQC (version 0.11.5)