FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053415
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCCCGGCTCTCAGGTTGTTCATTTGAAGATACA39460.3745912104915916No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT36210.34373917212114885No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA33920.32200035123859067No Hit
CTCCCAGACAGAGCCCCCAAACTCATCATTTATGAGGTCAGTAATCGGCCCTCAGGGGTTTCTGATCGCTTCT33380.31687416640165555No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG31980.3035840575651571No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC30540.28991423133333016No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG28770.2731117365900429No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28340.26902977459026123No Hit
GATTATTACTGCAGCTCATATACAAGCAGCATGACGGGCGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT26620.25270192659113455No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC26240.24909461133551355No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC26230.2489996819866814No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC25330.24045604059178957No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACGCCCGTCATGCTGCTTGTATATGAGCTGCAGTAATAAT25030.23760816012682562No Hit
GTGTATTACTGTGCAAGAGAGGGGCATAGTAGCAACTGGAATAACTGGTACTTCGATCTCTGGGGCCGTGGCACC24360.23124789375507276No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT24110.22887466003426948No Hit
GTCTGGGAGTTGTTGGTACCAGGAGACATAGTTATAACCACCAACGTCAC24080.22858987198777309No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC22820.21662877403492453No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACGCCCGTCATGCTGCTTGTATATGAGCTG21060.19992120864046936No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20690.19640882273368043No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC20420.1938457303152129No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19990.18976376831543124No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT19750.18748546394346008No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAACTCCCAGACAGAGC19360.18378321933900696No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACGCCCGTCATGCTGC19350.1836882899901748No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT19090.1812201269205394No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAACT18400.1746700018511223No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG17570.1667908658980554No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACGCCCGTCATG17490.16603143110739832No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG16980.1611900343169596No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG16970.16109510496812748No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT15710.14913400701527887No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15430.1464759852479792No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG15320.14543176241082573No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14920.14163458845754048No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG14860.1410650123645477No Hit
GGTTATAACTATGTCTCCTGGTACCAACAACTCCCAGACAGAGCCCCCAAACTCATCATTTATGAGGTCAGTAAT14850.14097008301571556No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC14250.13527432208578766No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA13680.12986334920235615No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13490.12805969157454564No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC12820.12169942520279281No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12570.11932619148198953No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT12510.11875661538899673No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12210.1159087349240328No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC12200.11581380557520064No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC11890.11287099576140457No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC11630.11040283269176915No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA11510.10926368050578356No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG11430.10850424571512653No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11240.10670058808731603No Hit
GCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCC11210.10641580004081962No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC11170.1060360826454911No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG11110.1054665065524983No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10980.1042324250176806No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT10900.10347299022702354No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10880.10328313152935928No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG10640.10100482715738812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTTC200.006638473453.11582656
TCGTATG1600.051.74765440
TACGTAT652.0082696E-550.3221870
CGTATGC1800.046.0733341
TCTCGTA1700.044.49934438
GACGCTC2150.044.3368926
GAGACGC2150.044.30952524
CGAGACG2150.044.30531723
ATCTCCG2150.044.294810
CGCTCAT2250.043.9128328
GTACGTA602.8215036E-643.3002569
TCTCCGA2200.043.28810511
GGTATCA9150.043.1314281
CCCACGA2300.041.40797419
ATCTCGT1850.040.8364237
CACGAGA2450.040.26111221
GTATGCC2250.040.01942
AGGGGGG3150.039.4589870
AGACGCT2500.038.1116225
ATGCCGT2100.036.37413844
Produced by FastQC (version 0.11.5)