FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131845
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57180.5051928488441438No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37790.3338796389965057No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37760.3336145850359369No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA37180.32849020846494No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT37140.3281368031841816No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35350.31232191687024285No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33340.29456330151213284No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33090.2923545185073928No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG30100.2659374737707018No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAAC27990.24729534521069582No Hit
GTCCCAGACCCACTGCCACTGAACCTGCCTGGGATGCCAGTGGCCCTGTT26700.23589802490623715No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTA25420.2245890559219681No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTTCGGCCAAGGGACACGACTGGA25310.22361719139988248No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24950.2204365438730568No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT24840.21946467935097122No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24550.2169024910654727No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24420.2157539239030079No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24300.2146937080607327No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24140.21328008693769906No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23730.2096576828099254No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23600.20850911564746058No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22740.2009109021111548No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22700.2005574968303964No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTA22680.20038079419001717No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22410.19799530854489794No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20770.1835056920338032No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20610.1820920709107696No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20490.18103185506849437No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGCCTGGGATGCCA20480.18094350374830476No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19670.173787046812947No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGTTGCTACGCTGCTGACAGTAATAAACTG18980.16769080571986447No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC18460.16309653707000515No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18350.16212467254791954No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT17760.15691194465673303No Hit
GTCGTGTCCCTTGGCCGAAGTTGCTACGCTGCTGACAGTAATAAACTGCA17290.15275943260782174No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC16980.1500205416819441No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16590.1465748401945496No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16320.14418935454943035No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16270.14374759794848235No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16190.14304078738696552No Hit
GTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG16130.14251067946582793No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15900.14047859910146707No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15890.14039024778127746No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT15690.13862322137748545No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15510.1370328976140726No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15470.1366794923333142No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15330.13544257385065978No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC15240.13464741196895336No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC15060.13305708820554052No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15010.1326153316045925No Hit
GTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC14920.13182016972288607No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14870.13137841312193807No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14410.1273142523932164No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA14380.12704919843264756No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13780.12174811922127146No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13640.12051120073861704No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13520.1194509848963418No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13490.11918593093577301No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA13420.11856747169444579No Hit
TTATTACTGTCAGCAGCGTAGCAACTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT13270.11724220189160177No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGGCAGGTT13250.11706549925122256No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13050.11529847284743053No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12950.11441495964553451No Hit
GAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC12750.11264793324174247No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12750.11264793324174247No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12600.11132266343889845No Hit
CAACAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG12560.11096925815814003No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12420.10973233967548561No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC12320.10884882647358958No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC12320.10884882647358958No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12270.10840706987264156No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG12210.10787696195150395No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12110.10699344874960794No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12000.10602158422752234No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11910.10522642234581592No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGGCTGG11820.1044312604641095No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT11750.10381280122278226No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11730.10363609858240308No Hit
GCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGCCTGGGATGCCAGTGG11680.10319434198145505No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11530.10186907217861103No Hit
TCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGC11500.10160401821804221No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11450.10116226161709421No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAAGTAGCTGCTA11450.10116226161709421No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11390.10063215369595659No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCGC207.3729534E-571.0341461
GGTATCA12100.043.951691
AATGGTA7950.040.5128170
TGCGGTA350.001367253739.31381236
GTATCAA32200.038.6432231
ATCAACG35550.034.9708063
TATCAAC35600.034.5466162
TCAACGC35800.034.530214
CAACGCA36400.034.053085
AACGCAG36900.033.776236
ACGCAGA39000.031.8701927
CGCAGAG39200.031.707598
AAAACCG1951.20926416E-831.6278470
AGAGTAC39900.031.14993911
GCAGAGT40100.030.9959459
GTGCTAG2700.027.7776361
CAGAGTA45900.027.0780510
AGTACGG33100.025.7198513
GAGTACG33250.025.6026912
CTCGCTA1101.278564E-624.79435723
Produced by FastQC (version 0.11.5)