FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1192712
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGTAGTTCTCCGAGGACGTTCGGCCAAGGGACCACGGTGGAGATCATACGAACTGTGGCTGCACCAT42840.35918142854268253No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40900.34291597636311194No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34850.29219124147321396No Hit
CTACCATACTGGTGACAGAAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC34330.287831429548793No Hit
GTTAGGACCCAGAGGGAACCATGGAAAGCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA29880.25052150058019035No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28070.235346001381725No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26540.22251809321948637No Hit
CCTCTATGCTACATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCTATGGGTCTGGGACAGACTTCA24240.20323430970762432No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23290.1952692686918552No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22770.1909094567674342No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22580.1893164485642804No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22440.18814265304616706No Hit
ATGCTACATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCTATGGGTCTGGGACAGACTTCACTCTCA22150.18571121947293226No Hit
CTCCTGCAGGGCCACTCAGAGTGTTAACGACGGCCAGGTAGCCTGGTACC21080.17674006801306602No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20670.1733025239957341No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAGGGTAATCTGCA20380.17087109042249932No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20090.16843965684926454No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19830.16625975088705403No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19630.1645829001468921No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18780.15745628450120397No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18680.156617859131123No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18500.1551086934649773No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18490.1550248509279692No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18190.1525095748177263No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTATGATCTCCACCGTGGTCC18100.15175499198465348No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17330.1452991166350301No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTCTATTTCTGTCACCAGTATGGTAGTTCT17070.1431192106728196No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16750.14043624948856054No Hit
GTGTATTTCTGTGCGAAGGAGGCGGGACGGGGAAACTGGGTCGCTGACTC16650.13959782411847957No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCACTCAGAGTGTTAACGA16400.13750176069327716No Hit
GATCAACACTGACAATGGTGACACAGATTATTCATGGAAGTTCCAGGGCAGACTCACCTTTACCAGGGACACATC16400.13750176069327716No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCACTCAGA16110.13507032712004238No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT15860.13297426369484No Hit
GTCTATTTCTGTCACCAGTATGGTAGTTCTCCGAGGACGTTCGGCCAAGGGACCACGGTGGAGATCATACGAA15740.13196815325074285No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC15680.13146509802869427No Hit
GTGTTAACGACGGCCAGGTAGCCTGGTACCAGCAGAGACCTGGCCAGCCTCCCAGGCTCCTCCTCTATGCTACAT15610.1308782002696376No Hit
GTCCCAGACCCATAGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT15580.13062667265861333No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15230.12769218386332995No Hit
GCAGAGACCTGGCCAGCCTCCCAGGCTCCTCCTCTATGCTACATCCAGCAGGGCCACTGGCATCCCAGACAGGTT15160.1271052861042733No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15110.1266860734192328No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15110.1266860734192328No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14930.1251769077530871No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGTTCCTGCCGG14840.1244223249200142No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14840.1244223249200142No Hit
GAAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCATAGCCA14790.12400311223497375No Hit
GGCTATGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTCTATTT14730.12350005701292517No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14670.1229970017908766No Hit
GAGTGAAGTCTGTCCCAGACCCATAGCCACTGAACCTGTCTGGGATGCCA14430.1209847809026823No Hit
GAAATACACAGCCGTGTCTTCAGATGTCAGACTGGTGAGGTCCATGTAGG14080.11805029210739894No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14050.11779876449637464No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14020.11754723688535036No Hit
GTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGC13960.11704418166330179No Hit
GTGCAGCCACAGTTCGTATGATCTCCACCGTGGTCCCTTGGCCGAACGTC13950.1169603391262937No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13800.11570270107117224No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC13670.114612748090067No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13560.11369048018297795No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13510.11327126749793748No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAAGCCCAGCGCAGCTTCTCT13490.11310358242392128No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13370.11209747197982413No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAAGCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13280.11134288914675128No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13250.11109136153572698No Hit
CTACATCCCTCCTCAGAAGCCCCCAGAGCACAACGCCTCACCATGGACTG12890.10807303020343552No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12820.10748613244437885No Hit
CTCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCT12770.10706691975933837No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12760.10698307722233028No Hit
GACCTGGCCAGCCTCCCAGGCTCCTCCTCTATGCTACATCCAGCAGGGCC12670.10622849438925742No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAGCGACCCAGTTTC12480.1046354861861036No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12470.10455164364909551No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12390.10388090335303074No Hit
GAGTGTTAACGACGGCCAGGTAGCCTGGTACCAGCAGAGACCTGGCCAGC12280.10295863544594168No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12260.1027909503719255No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12230.10253942276090122No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12130.10170099739082025No Hit
CCCATAGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGTAG12100.10144946977979595No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11970.1003595167986907No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG350.005165820556.58961570
GGTATCA9250.042.1403431
GTATCAA21700.034.82311
TAACGGC400.002639922534.3831536
CTCTTAT1351.0913936E-1130.3936181
TATCAAC25150.030.3142762
ATCAACG25700.029.5324953
TCAACGC26150.029.0242884
CGTATGC1800.028.78615241
CAACGCA26750.028.2454685
TATACAC2300.028.242693
ATGCCGT1850.028.09145744
ACGAACC755.5815053E-528.00487552
AACGCAG27250.027.7272036
GCGGTAC500.00757708627.71690244
TTAACGG500.00788357127.49260735
GACTAAC500.00808004127.3542561
GCCGTCT2050.027.10422546
CGAGACG1900.026.98980523
ACAGGTA1405.18412E-1026.865791
Produced by FastQC (version 0.11.5)