FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1192712
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGTAGTTCTCCGAGGACGTTCGGCCAAGGGACCACGGTGGAGATCATACGAACTGTGGCTGCACCAT41170.34517972486233056No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40130.3364601010134886No Hit
CTACCATACTGGTGACAGAAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC31480.2639363065014857No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29020.24331104239749413No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28620.2399573409171703No Hit
GTTAGGACCCAGAGGGAACCATGGAAAGCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28520.23911891554708933No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24310.20382120746668098No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23370.19594000898791997No Hit
CCTCTATGCTACATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCTATGGGTCTGGGACAGACTTCA23140.19401163063673377No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22410.18789112543514278No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21900.18361515604772988No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAGGGTAATCTGCA21580.1809321948634708No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20810.17447631951384743No Hit
ATGCTACATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCTATGGGTCTGGGACAGACTTCACTCTCA20640.1730509963847098No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20170.1691103971453293No Hit
CTCCTGCAGGGCCACTCAGAGTGTTAACGACGGCCAGGTAGCCTGGTACC19390.16257067925869784No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19040.15963619046341446No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18830.15787549718624447No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18020.15108425168858872No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17910.15016198378149964No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17890.14999429870748343No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17880.14991045617047535No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCACTCAGA17600.14756286513424868No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17440.14622138454211914No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTATGATCTCCACCGTGGTCC17080.14320305320982768No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17050.14295152559880342No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16910.14177773008069006No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16910.14177773008069006No Hit
GTGTATTTCTGTGCGAAGGAGGCGGGACGGGGAAACTGGGTCGCTGACTC16610.13926245397044718No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16540.1386755562113905No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCACTCAGAGTGTTAACGA16420.13766944576729334No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16340.13699870547122858No Hit
GTCTATTTCTGTCACCAGTATGGTAGTTCTCCGAGGACGTTCGGCCAAGGGACCACGGTGGAGATCATACGAA16330.1369148629342205No Hit
GTGTTAACGACGGCCAGGTAGCCTGGTACCAGCAGAGACCTGGCCAGCCTCCCAGGCTCCTCCTCTATGCTACAT16150.13540569726807478No Hit
GATCAACACTGACAATGGTGACACAGATTATTCATGGAAGTTCCAGGGCAGACTCACCTTTACCAGGGACACATC16140.13532185473106667No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTCTATTTCTGTCACCAGTATGGTAGTTCT15970.13389653160192905No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT15950.13372884652791286No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15780.13230352339877524No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC14700.12324852940190087No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14620.12257778910583611No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14510.12165552119874706No Hit
GCAGAGACCTGGCCAGCCTCCCAGGCTCCTCCTCTATGCTACATCCAGCAGGGCCACTGGCATCCCAGACAGGTT14350.12031404060661752No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14330.12014635553260133No Hit
GTCCCAGACCCATAGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT14110.11830181971842321No Hit
GGCTATGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTCTATTT13960.11704418166330179No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13900.11654112644125321No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13600.11402585033101033No Hit
GTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGC13590.11394200779400224No Hit
GAAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCATAGCCA13480.11301973988691319No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13340.11184594436879985No Hit
GTGCAGCCACAGTTCGTATGATCTCCACCGTGGTCCCTTGGCCGAACGTC13340.11184594436879985No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC13160.11033677870265413No Hit
CTCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCT12800.10731844737036267No Hit
GTGTTGATCCATCCCATCCACTCAAGGCTTTGTCCGGGGGCCTGGCGCAC12700.1064800220002817No Hit
GAGTGAAGTCTGTCCCAGACCCATAGCCACTGAACCTGTCTGGGATGCCA12680.10631233692626552No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12620.10580928170421694No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12570.10539006901917645No Hit
GAGTGTTAACGACGGCCAGGTAGCCTGGTACCAGCAGAGACCTGGCCAGC12530.10505469887114409No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12470.10455164364909551No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12450.10438395857507932No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAAGCCCAGCGCAGCTTCTCT12450.10438395857507932No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12340.10346169066799026No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAAGCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC12330.10337784813098216No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12280.10295863544594168No Hit
GACCTGGCCAGCCTCCCAGGCTCCTCCTCTATGCTACATCCAGCAGGGCC12230.10253942276090122No Hit
GAAATACACAGCCGTGTCTTCAGATGTCAGACTGGTGAGGTCCATGTAGG12190.10220405261286883No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12150.10186868246483644No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12140.10178483992782834No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12040.10094641455774737No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11970.1003595167986907No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAGCGACCCAGTTTC11960.10027567426168262No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11950.10019183172467452No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)