FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1204028
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAGGCTGCTCATTTACTGGACTTCTACCCGGGTATCCGGGGTCCCT82440.6847016846784294No Hit
GCCTAGGAGGCTGTCCTGGTTTCTGCTGATACCAGGCTAAGTAGTTCTTA68230.5666811735275259No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60490.5023969542236559No Hit
CTTTTATACACCTCCAACAATAAGAACTACTTAGCCTGGTATCAGCAGAA53270.44243157135880556No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52160.43321251665243665No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43620.3622839335962287No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39920.3315537512416655No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35810.2974183324640291No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33190.2756580411751222No Hit
GTTTATTACTGTCAGCAATATTATTATCCTCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA33100.27491055025298416No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32540.27025949562634755No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31790.2640304046085307No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31420.26095738637307436No Hit
CACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATTATCCTCCACTCACTTT29900.24833309524363223No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29110.24177178603819846No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28170.23396465862920132No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28140.23371549498848865No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28030.2328018949725422No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27540.22873222217423517No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26850.22300145843784364No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG26600.220925094765238No Hit
AACCAGGACAGCCTCCTAGGCTGCTCATTTACTGGACTTCTACCCGGGTA25390.21087549458982682No Hit
GTATAAAAGATTGTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA25370.2107093854960184No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC24910.20688887633842404No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCACAATCTTTTATA24790.20589222177557331No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA24640.20464640357200997No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24150.20057673077370294No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC23500.19517818522492833No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATTATCCT22510.18695578508141006No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCACAATCTTTTATACACCTCCAA22050.18313527592381573No Hit
TTATTATCCTCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT22020.18288611228310306No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21730.18047753042288053No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21650.17981309404764673No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC21490.1784842212971791No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20780.17258734846697918No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20470.1700126575129482No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC20190.1676871301996299No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20160.1674379665589172No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20040.16644131199606654No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20010.16619214835535387No Hit
ATATTATTATCCTCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT19920.16544465743321585No Hit
GTCAGGGACCCCGGATACCCGGGTAGAAGTCCAGTAAATGAGCAGCCTAGGAGGCTGTCCTGGTTTCTGCTGATA19760.16411578468274823No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCACCAGCC19340.1606274937127708No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18770.15589338453922996No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18090.1502456753497427No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17830.14808625713023285No Hit
GTCCTGGTTTCTGCTGATACCAGGCTAAGTAGTTCTTATTGTTGGAGGTG17380.14434880251954274No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17200.1428538206752667No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16810.1396146933460019No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16800.1395316387990977No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16500.13704000239197095No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16410.13629251146983293No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCACAATCTTTTATACACCTCCAACAATAAGAACTA16400.1362094569229287No Hit
TCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATAT16340.13571112964150336No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT16140.13405003870341886No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16040.1332194932343766No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15930.13230589321843014No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15780.13106007501486677No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15410.12798705677941044No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15300.127073456763464No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15110.1254954203722837No Hit
ACTTAGCCTGGTATCAGCAGAAACCAGGACAGCCTCCTAGGCTGCTCATT15050.12499709309085835No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15000.12458182035633722No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14950.12416654762181611No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT14850.12333600215277385No Hit
GAACTACTTAGCCTGGTATCAGCAGAAACCAGGACAGCCTCCTAGGCTGC14780.12275462032444429No Hit
GGATAATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAAT14760.12258851123063584No Hit
GCCTCCTAGGCTGCTCATTTACTGGACTTCTACCCGGGTATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGT14750.12250545668373161No Hit
CCTCCAACAATAAGAACTACTTAGCCTGGTATCAGCAGAAACCAGGACAGCCTCCTAGGCTGCTCATTTACTGGA14710.12217323849611471No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14630.1215088021208809No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG14520.12059520210493443No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG14480.12026298391731753No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14460.12009687482350909No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14400.11959854754208374No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14350.1191832748075626No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14110.11718996568186121No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14110.11718996568186121No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13970.11602720202520207No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA13920.11561192929068094No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13910.11552887474377672No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATTATCCTCCAC13810.11469832927473447No Hit
TAATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC13740.11411694744640491No Hit
GTCTGGGACAGATTTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATG13590.11287112924284154No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13300.110462547382619No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13300.110462547382619No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13030.10822007461620495No Hit
TTATTACTGTCAGCAATATTATTATCCTCCACTCACTTTCGGCGGAGGGA12720.10564538366217398No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12700.10547927456836552No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12650.10506400183384439No Hit
CAATAAGAACTACTTAGCCTGGTATCAGCAGAAACCAGGACAGCCTCCTAGGCTGCTCATTTACTGGACTTCTAC12600.10464872909932328No Hit
CTACTTAGCCTGGTATCAGCAGAAACCAGGACAGCCTCCTAGGCTGCTCATTTACTGGACTTCTACCCGGGTATC12310.10224014723910074No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC200.00793839550.767573
TCGTATG1500.048.27322840
CGTATGC1750.045.3887841
GTGGTAT2550.042.470451
ACGAGAC1850.042.10385522
ATCTCGT1550.039.82158337
GATTGTG27650.039.6308870
CGACCGG1300.039.05359611
GTATCAA26550.038.113931
CCGTCTT1850.037.72839447
AGTATAC451.1212395E-437.6056063
ATGCCGT2100.036.3907444
ATCTCCG2050.034.67197410
TCTCCGA2050.034.67197411
CCCACGA2150.034.6393319
CACGAGA2200.033.85910821
TGCACCG1500.033.847866
GCCCACG2200.033.84785518
GGTCTGG34800.033.80648870
TATCAAC30500.033.7340772
Produced by FastQC (version 0.11.5)