FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267769
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11680.43619687118374423No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10670.39847779242556086No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8560.31967852888123716No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8520.318184703979923No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8440.3151970541772946No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACAAATTAGAA8420.31445014172663754No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7640.28532055615101076No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7450.27822488786976834No Hit
GAGTTCACACTCACCATCAGGTACCTGCAGCCTGATGATTTTGCAACTTA7200.2688884822365546No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC7170.26776811356056895No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7120.26590083243392626No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6810.25432368944874123No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6760.25245640832209854No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6430.24013235288625642No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6400.23901198421027078No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT6260.23378359705567114No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6060.22631447254910014No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG5990.22370027897180034No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG5830.21772497936654356No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5590.2087620299586584No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT5530.2065212926066871No Hit
AATTTAGAGAGTGGGGTCCCACCAAGGTTCAGCGGCAGTGGGTCTGCGACAGAGTTCACACTCACCATCAGGTAC5390.20129290545208745No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5220.1949441496215021No Hit
GTCTGCATCTGTAGGAGACACAGTCACCCTCACTTGCCGGGCCAGTCAGA5100.19046267491755955No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACACAGTCACCCTCACTTGCCG5090.19008921869223097No Hit
GTGTGAACTCTGTCGCAGACCCACTGCCGCTGAACCTTGGTGGGACCCCA4960.18523428776295986No Hit
CCTTATAAATGAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACAAATTAGAAACACTCTGA4950.18486083153763133No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4690.17515096967908905No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT4630.17291023232711777No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG4590.17141640742580358No Hit
GTAATACGTGGCTGTGTCTACCGGCTCCATGTTGGTCATTGTAAGGACCA4580.17104295120047502No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4580.17104295120047502No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG4510.1684287576231752No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA4500.16805530139784666No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC4460.16656147649653244No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT4390.16394728291923263No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT4320.1613330893419328No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT4200.15685161463799022No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT4180.1561047021873331No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC4160.15535778973667602No Hit
CTCTAAATTAGACGCCTTATAAATGAGGAGTTTAGGGGCTTTCCCTGGTT4130.15423742106069036No Hit
CCCTAAGGAGACGCTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGAGCCCGGAATTCCAGTCACTGGAATAGCC4100.15311705238470472No Hit
ACCTTGGTGGGACCCCACTCTCTAAATTAGACGCCTTATAAATGAGGAGTTTAGGGGCTTTCCCTGGTTTCTG3980.14863557768076213No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC3910.14602138410346233No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA3870.14452755920214813No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT3850.14378064675149102No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC3840.1434071905261625No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG3810.14228682185017685No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC3770.14079299694886263No Hit
CAGTAATACGTGGCTGTGTCTACCGGCTCCATGTTGGTCATTGTAAGGAC3680.1374318909209057No Hit
TTTATAAGGCGTCTAATTTAGAGAGTGGGGTCCCACCAAGGTTCAGCGGCAGTGGGTCTGCGACAGAGTTCACA3650.13631152224492005No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC3630.13556460979426296No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC3620.1351911535689344No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT3600.1344442411182773No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG3590.13407078489294877No Hit
CCATCAGGTACCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAA3530.1318300475409775No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC3500.13070967886499182No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA3470.12958931018900618No Hit
GTAGGAGACACAGTCACCCTCACTTGCCGGGCCAGTCAGAGTGTTTCTAATTTGTTGGCCTGGTATCAGCAGAAA3470.12958931018900618No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA3450.1288423977383491No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG3430.12809548528769202No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT3430.12809548528769202No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT3420.12772202906236343No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG3420.12772202906236343No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC3410.1273485728370349No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG3230.12062636078112104No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG3190.11913253587980685No Hit
CCCCTAAACTCCTCATTTATAAGGCGTCTAATTTAGAGAGTGGGGTCCCACCAAGGTTCAGCGGCAGTGGGTCTG3140.1172652547531641No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT3140.1172652547531641No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG3080.11502451740119282No Hit
GTGCAGCCACAGTTCGTTCGATTTCCACCTTGGTCCCCTGGCCGAACGTC3060.11427760495053571No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA3030.11315723627455007No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC2950.1101695864719217No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA2940.10979613024659314No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC2910.10867576157060749No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT2860.10680848044396477No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACACAGTCACCCTCACTT2840.10606156799330767No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT2800.10456774309199347No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA2740.1023270057400222No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC2730.10195354951469363No Hit
CACCATCAGGTACCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAATATAGTAATTTTTTGACGTTCGG2710.10120663706403654No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA2710.10120663706403654No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG2700.10083318083870799No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA2700.10083318083870799No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG2700.10083318083870799No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT2690.10045972461337944No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAATTG200.005090594856.7892768
AGCGTAC200.007527115351.44445
TAACTCA200.007527115351.44444
GTATCAA4700.043.7824671
GCTCGGG903.710933E-641.5747670
GTTCTAG350.001385730839.1957361
GCAGCGT1502.3173925E-937.41728670
TATCAAC5450.037.1280672
ATCAACG5800.035.4788973
TCAACGC5900.034.877564
CAACGCA6000.034.296275
AATGGTA1655.8844307E-934.01571370
AACGCAG6100.033.734036
ACGCAGA6450.031.9035027
ACTGGTC450.004734783430.5083626
GAGCAAA4550.030.15312470
AGAGTAC6900.029.8228411
CGCAGAG6900.029.822848
CAGAGTA6900.029.8228410
GCAGAGT7100.028.9827619
Produced by FastQC (version 0.11.5)