FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267769
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10560.3943697739469468No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10030.37457659400453375No Hit
GTGTGAACTCTGTCGCAGACCCACTGCCGCTGAACCTTGGTGGGACCCCA9630.35963834499139186No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8560.31967852888123716No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8120.3032464549667811No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8070.30137917384013835No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7790.290922399530939No Hit
GAGTTCACACTCACCATCAGGTACCTGCAGCCTGATGATTTTGCAACTTA7630.2849470999256822No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7410.2767310629684541No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACAAATTAGAA7080.26440700753261204No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7050.2632866388566264No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6770.25282986454742706No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6480.24199963401289915No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC6350.23714470308362806No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG6340.2367712468582995No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT6160.23004903480238564No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6150.22967557857705706No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5670.21174967976128675No Hit
CCCTAAGGAGACGCTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGAGCCCGGAATTCCAGTCACTGGAATAGCC5580.20838857373332983No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG5550.2072682050573442No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG5390.20129290545208745No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5380.2009194492267589No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG5300.19793179942413053No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5200.19419723717084503No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA5020.18747502511493117No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT4880.18224663796033147No Hit
AATTTAGAGAGTGGGGTCCCACCAAGGTTCAGCGGCAGTGGGTCTGCGACAGAGTTCACACTCACCATCAGGTAC4790.17888553193237455No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC4660.1740306010031034No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA4640.17328368855244633No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4610.17216331987646066No Hit
GTCTGCATCTGTAGGAGACACAGTCACCCTCACTTGCCGGGCCAGTCAGA4510.1684287576231752No Hit
CCTTATAAATGAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACAAATTAGAAACACTCTGA4480.16730838894718955No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA4400.16432073914456116No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT4360.16282691424324697No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG4290.16021272066594716No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACACAGTCACCCTCACTTGCCG4190.15647815841266166No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC4150.15498433351134747No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC4020.15012940258207635No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC3990.14900903390609072No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT3970.1482621214554336No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG3860.14415410297681958No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3810.14228682185017685No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT3790.14153990939951974No Hit
TTTATAAGGCGTCTAATTTAGAGAGTGGGGTCCCACCAAGGTTCAGCGGCAGTGGGTCTGCGACAGAGTTCACA3750.14004608449820555No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC3740.139672628272877No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT3630.13556460979426296No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA3620.1351911535689344No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG3610.13481769734360588No Hit
GTAATACGTGGCTGTGTCTACCGGCTCCATGTTGGTCATTGTAAGGACCA3580.13369732866762024No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT3570.13332387244229166No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC3550.13257695999163457No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC3470.12958931018900618No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC3380.12622820416104927No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC3350.12510783548506363No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC3310.12361401058374943No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGTACCTGATGGTGAGTGTGAACTCTGTCGCAGACCCACTGCCG3310.12361401058374943No Hit
CTCTAAATTAGACGCCTTATAAATGAGGAGTTTAGGGGCTTTCCCTGGTT3160.11801216720382121No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG3130.11689179852783557No Hit
CCATCAGGTACCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAA3120.116518342302507No Hit
GTGCAGCCACAGTTCGTTCGATTTCCACCTTGGTCCCCTGGCCGAACGTC3110.11614488607717846No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT3080.11502451740119282No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA3060.11427760495053571No Hit
GTCGCAGACCCACTGCCGCTGAACCTTGGTGGGACCCCACTCTCTAAATT3050.11390414872520717No Hit
ACCTTGGTGGGACCCCACTCTCTAAATTAGACGCCTTATAAATGAGGAGTTTAGGGGCTTTCCCTGGTTTCTG3050.11390414872520717No Hit
CCCCTAAACTCCTCATTTATAAGGCGTCTAATTTAGAGAGTGGGGTCCCACCAAGGTTCAGCGGCAGTGGGTCTG3030.11315723627455007No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT2990.11166341137323589No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA2950.1101695864719217No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT2860.10680848044396477No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG2850.10643502421863621No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC2850.10643502421863621No Hit
CAGTAATACGTGGCTGTGTCTACCGGCTCCATGTTGGTCATTGTAAGGAC2830.10568811176797913No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC2810.10494119931732201No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC2770.10344737441600783No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT2760.10307391819067926No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC2700.10083318083870799No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)