FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799256
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTACAGTGCCCCTTTAATCACTTTCGGCCCTGGGACCAAAGTGCATATGAAACGAACTGTGGCTGCACCAT38150.4773189065831223No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28160.3523276647281972No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27270.34119230884722795No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23790.29765181618905584No Hit
CTGTAACCCTGTTGACAGTAATAAGTTGCAATGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAATTCTGTC21700.2715024973225099No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20990.26261923588937713No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20630.25811504699370413No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19050.2383466623960283No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16630.20806850370844887No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16510.2065671074098912No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14770.18479686108080515No Hit
GATCTATGGTGCATCCCGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCA14280.17866615952836137No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14250.17829081045372194No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13940.17441220334911467No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13580.1699080144534417No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13560.16965778173701543No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATTAGTAGCTATTTAATTTGGTATCAGCAGAAA13510.16903219994594973No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13410.16778103636381833No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13310.16652987278168696No Hit
GTAATATACGGCCGTGTCCTCGGCTGTCAGGCTGCTCATTTGCAAGTACA12970.16227591660244026No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12970.16227591660244026No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12320.15414335331858628No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11440.1431331137958301No Hit
GAATTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACATTGCAACTTATTACTGTCAACAGGGTTACAGTGCC11340.14188195021369873No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11300.1413814847808462No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11120.1391293903330097No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10660.13337403785520532No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10660.13337403785520532No Hit
GTCTCCATCCTCCCTGTCTGCCTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATTAGTAG10640.13312380513877908No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10350.12949543075059805No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9530.11923588937712071No Hit
CCATAGATCAGGAGCTTAGGGCCTTTCCCTGGTTTCTGCTGATACCAAAT9460.11836007486962877No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9370.1172340276457105No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9300.11635821313821855No Hit
GTATATTACTGTGCGAGAGAGTTTAACTATGGTGACTTCCAGTTTGACTA9250.11573263134715284No Hit
GTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGAC8850.11072797701862733No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT8670.10847588257079084No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATATGCACTTTGGTCC8660.10835076621257769No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8620.10785030077972516No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8510.10647402083938064No Hit
GTGCAGCCACAGTTCGTTTCATATGCACTTTGGTCCCAGGGCCGAAAGTG8480.1060986717647412No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8470.10597355540652806No Hit
CCTGAAGACATTGCAACTTATTACTGTCAACAGGGTTACAGTGCCCCTTT8450.1057233226901018No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATTAGT8400.10509774089903612No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8370.10472239182439669No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8270.10347122824226532No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC8250.10322099552583902No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8150.10196983194370765No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8040.10059355200336313No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)