FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187403
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65180.5489290493623479No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56780.4781864287019656No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43580.36701945337850755No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42180.35522901660177714No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37810.3184260103772687No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG37420.3151415315608938No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC35990.3030984425675192No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35690.30057192040107694No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35430.29838226785682703No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33660.2834757870748179No Hit
GACCAAGGTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT31780.26764291483177993No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30670.2582947828159437No Hit
GTATATTACTGTGCGAGAGCACAACAGGATCTTCTAAGGGAATATCGCTACTACTATGAATACGGGATGGACGT30550.2572841739493668No Hit
GTGCAGCCACAGTTCGTTTGAGCTCCACCTTGGTCCCTCCGCCGAAAGCG30340.25551560843285726No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG30170.25408391253854No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29790.25088365112771316No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28460.2396827361898193No Hit
GGCTATCAGTTTCCCCCTCGCTTTCGGCGGAGGGACCAAGGTGGAGCTCAAACGAACTGTGGCTGCACCAT28270.2380826054844059No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28240.23782995326776168No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27620.23260847412378108No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27100.22822916903528118No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT26690.22477625540781015No Hit
ACCTTGGTCCCTCCGCCGAAAGCGAGGGGGAAACTGATAGCCCGTTGACAATAGTAAGTTGCAAAATCTTCAGG26420.22250238545801215No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25910.21820729777506037No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25890.21803886296396421No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24820.20902760057032024No Hit
GGATATTAGGAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTTTACTGCATC24400.20549046953730116No Hit
GTAGGAGACACCGTCACCATCACCTGTCGGGCGAGTCGGGATATTAGGAGCTGGTTAGCCTGGTATCAGCAGAAA24090.20287972996531084No Hit
GTAATATACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTCCAGGTACA23220.1955528156826284No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22490.18940494507761896No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCTCCACCTTGGTCCCTCCGCCGAAAGCGAGGGGGAAA22030.18553094442240756No Hit
CTGATAGCCCGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGTTGAGAGTGAATTCTGTC21480.18089898711726346No Hit
GGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATC21130.17795137792308088No Hit
CTGTAGGAGACACCGTCACCATCACCTGTCGGGCGAGTCGGGATATTAGG21050.17727763867869628No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20810.1752564209455425No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20740.174666899106706No Hit
TCTTTACTGCATCCAATTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT19970.16818215887950427No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19910.16767685444621583No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19900.16759263704066774No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19530.164476593035389No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19320.16270802751887944No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19290.16245537530223522No Hit
GAATTCACTCTCAACATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACGGGCTATCAGTTTC18980.1598446357302449No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18080.15226506923091823No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18060.15209663441982207No Hit
GTCTCCATCTTCCGTGTCTGCGTCTGTAGGAGACACCGTCACCATCACCTGTCGGGCGAGTCGGGATATTAGGAG17400.14653828565364918No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17320.1458645464092646No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC17210.14493815494823575No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17170.14460128532604347No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17110.14409598089275502No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16680.14047463245418784No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16490.13887450174877444No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16480.13879028434322635No Hit
GATCTTTACTGCATCCAATTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA16460.1386218495321302No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16270.1370217188267168No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCAACATCAGCAGCCTGCAGCCTGAAGATTTTG16220.1366006317989764No Hit
ACCTTGATGGGACCCCACTTTGTAAATTGGATGCAGTAAAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG15790.13297928336040923No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15730.1324739789271208No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15700.13222132671047657No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15450.1301158915717747No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15400.12969480454403434No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15280.12868419567745745No Hit
CATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACGGGCTATCAGTTTCCCCCTCGCTTTCGG15160.12767358681088056No Hit
CCTAATATCCCGACTCGCCCGACAGGTGATGGTGACGGTGTCTCCTACAG15140.1275051519997844No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14980.12615767351101523No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14820.12481019502224604No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14790.1245575428056018No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14640.1232942817223807No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC14430.12152571620587113No Hit
CAACATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACGGGCTATCAGTTTCCCCCTCGCTTT14420.12144149880032305No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14360.12093619436703461No Hit
ATTAGGAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA14250.12000980290600578No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14180.11942028106716929No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14120.11891497663388084No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14060.1184096722005924No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13660.11504097597866941No Hit
CAGTAAAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACCAGCTCCTAATATCCCGA13650.11495675857312133No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC13500.11369349748990022No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13410.11293554083996757No Hit
CCCCTAAGCTCCTGATCTTTACTGCATCCAATTTACAAAGTGGGGTCCCA13350.11243023640667912No Hit
GAGTCAGACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTT13180.11099854051236184No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13170.11091432310681379No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12940.10897732277920806No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12890.1085562357514677No Hit
ATCTGGGACAGAATTCACTCTCAACATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACGGG12880.10847201834591962No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12880.10847201834591962No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12820.10796671391263117No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACCAGCTCCTA12520.10544019174618896No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12420.1045980176907082No Hit
TTACTATTGTCAACGGGCTATCAGTTTCCCCCTCGCTTTCGGCGGAGGGA12260.10325053920193902No Hit
GGACCAAGGTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12150.10232414774091021No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTTTACTGCATCCAATTTACAAAGTGGGGTCCCAT12050.10148197368542947No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12040.1013977562798814No Hit
GTATCAACGCAGAGTACGGGGCAGGAGTCAGACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCT12010.10114510406323717No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11940.10055558222440064No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT250.00136956679.10123470
CGTCGAA252.8001977E-454.23767514
GTGGTAT1550.045.9212651
CGTATGC1700.044.74302741
AATGGTA5400.043.94513370
TCGTATG1750.043.3956940
ATCTCGT1100.040.57108737
GCCGTCT1850.039.5624246
GTAATAC451.11168E-437.6602943
GTCGTAT557.4752334E-637.57684339
GTATCAA18650.037.438171
TCTGGAG8100.036.6209470
ACGAGAC2050.036.39425722
ATGCCGT2100.036.38150844
CTTATAC2350.036.057731
GAGCGAT1800.035.98487532
ATCTCCG2000.035.59047710
TTATACA2400.035.3065262
CACGAGA2300.033.9099721
TGTATGC502.0656115E-433.8942645
Produced by FastQC (version 0.11.5)