FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187403
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT66790.5624880516555878No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47210.39759037159245847No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45370.3820943689716128No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45290.38142062972722823No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39530.33291140413153747No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC34320.2890341358409908No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33520.2822967433971449No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32860.276738394630972No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32660.2750540465200105No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32420.2730328287868567No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31690.26688495818184726No Hit
GTGCAGCCACAGTTCGTTTGAGCTCCACCTTGGTCCCTCCGCCGAAAGCG30730.25880008724923215No Hit
GACCAAGGTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT30180.25416812994408805No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29260.24642012863366525No Hit
GTATATTACTGTGCGAGAGCACAACAGGATCTTCTAAGGGAATATCGCTACTACTATGAATACGGGATGGACGT29110.24515686755044414No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28050.23622982256234826No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27620.23260847412378108No Hit
GGCTATCAGTTTCCCCCTCGCTTTCGGCGGAGGGACCAAGGTGGAGCTCAAACGAACTGTGGCTGCACCAT27510.23168208266275223No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27240.22940821271295425No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27160.22873447346856965No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG26820.22587108167993514No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26360.22199708102472368No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT26140.22014429810266606No Hit
ACCTTGGTCCCTCCGCCGAAAGCGAGGGGGAAACTGATAGCCCGTTGACAATAGTAAGTTGCAAAATCTTCAGG24850.2092802527869645No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24260.2043114258596281No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT24120.20313238218195506No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23970.20186912109873395No Hit
GTAATATACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTCCAGGTACA22790.19193146724406118No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCTCCACCTTGGTCCCTCCGCCGAAAGCGAGGGGGAAA22550.1899102495109074No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22390.18856277102213823No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22060.18578359663905178No Hit
GGATATTAGGAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTTTACTGCATC21860.1840992485280903No Hit
GTAGGAGACACCGTCACCATCACCTGTCGGGCGAGTCGGGATATTAGGAGCTGGTTAGCCTGGTATCAGCAGAAA21810.18367816150034993No Hit
CTGATAGCCCGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGTTGAGAGTGAATTCTGTC21670.1824991178226769No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21390.18014103046733082No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21120.1778671605175328No Hit
GAATTCACTCTCAACATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACGGGCTATCAGTTTC19410.1634659841688121No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19410.1634659841688121No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18940.15950776610805262No Hit
GGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATC18880.15900246167476417No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18880.15900246167476417No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18780.15816028761928344No Hit
CTGTAGGAGACACCGTCACCATCACCTGTCGGGCGAGTCGGGATATTAGG18680.1573181135638027No Hit
TCTTTACTGCATCCAATTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT18130.1526861562586586No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17870.15049650371440867No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17510.147464677114678No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17220.14502237235378385No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17000.14316958943172622No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16160.13609532736568797No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16080.13542158812130337No Hit
GATCTTTACTGCATCCAATTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA16070.13533737071575533No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC15730.1324739789271208No Hit
GTCTCCATCTTCCGTGTCTGCGTCTGTAGGAGACACCGTCACCATCACCTGTCGGGCGAGTCGGGATATTAGGAG15570.13112650043835158No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCAACATCAGCAGCCTGCAGCCTGAAGATTTTG15520.13070541341061123No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15500.13053697859951507No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15470.13028432638287085No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT15270.12859997827190936No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15240.12834732605526517No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15000.12632610832211136No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14930.12573658648327485No Hit
ACCTTGATGGGACCCCACTTTGTAAATTGGATGCAGTAAAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG14720.12396802096676528No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14670.12354693393902491No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14570.12270475988354418No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14450.12169415101696728No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14340.12076775955593846No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14260.12009402031155386No Hit
CCTAATATCCCGACTCGCCCGACAGGTGATGGTGACGGTGTCTCCTACAG14120.11891497663388084No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13990.11782015036175587No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13900.11706219371182319No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13770.11596736743969822No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13770.11596736743969822No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT13750.1157989326286021No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13710.11546206300640978No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC13650.11495675857312133No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13570.11428301932873675No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA13320.11217758419003489No Hit
CATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACGGGCTATCAGTTTCCCCCTCGCTTTCGG13160.11083010570126571No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12970.1092299749958523No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12960.10914575759030422No Hit
CAGTAAAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACCAGCTCCTAATATCCCGA12850.10821936612927541No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12760.10746140947934274No Hit
CAACATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACGGGCTATCAGTTTCCCCCTCGCTTT12710.10704032245160236No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGTAAATT12640.10645080061276585No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12590.10602971358502547No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12480.10510332212399665No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACCAGCTCCTA12460.10493488731290052No Hit
GTATCAACGCAGAGTACGGGGCAGGAGTCAGACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCT12440.10476645250180436No Hit
CCCCTAAGCTCCTGATCTTTACTGCATCCAATTTACAAAGTGGGGTCCCA12290.10350319141858323No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12160.10240836514645829No Hit
GAGTCAGACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTT12050.10148197368542947No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)