FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069464
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51880.48510281786016174No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51230.479025006919354No Hit
GTAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT37120.3470897571119738No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36400.34035741268523295No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32220.30127241309665403No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31240.2921089442935901No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC29780.2784572458726989No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29700.277709207603061No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGGTACCTAGAGCCTGAAGATT29640.2771481789008326No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATAA29590.2766806549823089No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29420.27509107365932844No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29000.2711638727437296No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28760.268919757934816No Hit
GTGTTGACACAGTCTCCAGCCACCATGTCTTTGTCTCCAGGGGACACAGC28430.2658341000725597No Hit
GTTTACTACTGTCAGTCTCGTTACGACTGGCCGACGTTCGGCCAAGGGAC26830.2508733346798022No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26570.24844221030347913No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25820.24142935152562406No Hit
CGTTACGACTGGCCGACGTTCGGCCAAGGGACCAAGGTGGAAAGTAAAGG25530.23871771279818677No Hit
GTTCCTTTACTTTCCACCTTGGTCCCTTGGCCGAACGTCGGCCAGTCGTA25520.23862420801448203No Hit
GTCGTAACGAGACTGACAGTAGTAAACTGCAAAATCTTCAGGCTCTAGGTACCTGATGGTGAGAGTGAAGT24910.23292041620849324No Hit
CTCCTGCAGGGCCAGTCGGAGTATTGGCAACTACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGACT24170.2260010622143429No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24130.22562704307952397No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22910.21421945946754634No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22660.21188183987492798No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTACTTTCCACCTTGGTCC21580.20178332323481668No Hit
CTACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGACTCGTCGTCTATGATGCATCGAACAGGGCCG21310.19925869407478886No Hit
GACTTCACTCTCACCATCAGGTACCTAGAGCCTGAAGATTTTGCAGTTTACTACTGTCAGTCTCGTTACGACTGG21070.19701457926587523No Hit
GTAGTAAACTGCAAAATCTTCAGGCTCTAGGTACCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20660.19318088313398113No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20510.19177831137841012No Hit
GTCCCAGACCCACTGCCACTGAACCTGGTTGGGACGCCAGCGGCCCTGTT20430.19103027310877224No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20150.18841213916503968No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18910.1768175459856526No Hit
GAACAGGGCCGCTGGCGTCCCAACCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGGTA18400.17204880201671116No Hit
CAGCCACCCTCTCCTGCAGGGCCAGTCGGAGTATTGGCAACTACTTAGCCTGGTACCAACAAAAACCTGGCCAGG17930.16765407718258865No Hit
CTCCCAGACTCGTCGTCTATGATGCATCGAACAGGGCCGCTGGCGTCCCA17770.1661580006433129No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17610.16466192410403716No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17440.16307234278105667No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16960.15858411316322943No Hit
GAGTATTGGCAACTACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTC16650.15568546486838267No Hit
GTCTCCAGCCACCATGTCTTTGTCTCCAGGGGACACAGCCACCCTCTCCTGCAGGGCCAGTCGGAGTATTGGCAA16190.1513842448179649No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16130.15082321611573649No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGTTGGGACGCCAGCGGCCCTGTTCGATGCATCATAGA15850.14820508217200393No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15560.14549344344456663No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15450.14446489082381456No Hit
GATATAACCGGAGACATTGTGTTGACACAGTCTCCAGCCACCATGTCTTTGTCTCCAGGGGACACAGCCACCCT15360.14362334777047195No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15080.1410052138267394No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14730.1377325463970737No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14650.13698450812743582No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14590.1364234794252074No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14580.13632997464150268No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT14570.13623646985779794No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14470.1353014220207506No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14380.134459878967408No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14360.1342728693999985No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14030.13118721153774227No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13930.13025216370069492No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13720.1282885632428955No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13380.12510940059693454No Hit
GGTACCTAGAGCCTGAAGATTTTGCAGTTTACTACTGTCAGTCTCGTTACGACTGGCCGACGTTCGGCCAAGGGA13230.12370682884136353No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13130.12277178100431618No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13070.12221075230208778No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12970.12127570446504043No Hit
GGACCAAGGTGGAAAGTAAAGGAACTGTGGCTGCACCATCTGTCTTCATC12960.1211821996813357No Hit
CTGCTACTCTGGCTCCCAGATATAACCGGAGACATTGTGTTGACACAGTC12940.12099519011392623No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12880.12043416141169783No Hit
GTGCAGCCACAGTTCCTTTACTTTCCACCTTGGTCCCTTGGCCGAACGTC12870.12034065662799309No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12870.12034065662799309No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12810.11977962792576469No Hit
GCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGACTCGTCGTCTATGATGCATCGAACAGGGCCGCTGGCGTC12760.11931210400724102No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12620.11800303703537474No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG12380.11575892222646111No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12130.11342130263384276No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12010.11229924522938595No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11860.11089667347381493No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA11830.11061615912270073No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11740.1097746160693581No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11600.10846554909749181No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11450.1070629773419208No Hit
ATGATGCATCGAACAGGGCCGCTGGCGTCCCAACCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCA11360.10622143428857819No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC11310.10575391037005452No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC11280.10547339601894032No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11280.10547339601894032No Hit
CTAGGTACCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGTTGGGACGCCAGCGGCC11210.10481886253300718No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11200.10472535774930244No Hit
ACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGACTCGTCGTC11130.1040708242633693No Hit
ACAAAAACCTGGCCAGGCTCCCAGACTCGTCGTCTATGATGCATCGAACAGGGCCGCTGGCGTCCCAACCAGGTT11070.1035097955611409No Hit
CCTTGGTCCCTTGGCCGAACGTCGGCCAGTCGTAACGAGACTGACAGTAG11020.10304227164261723No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG10920.10210722380556989No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10850.10145269031963675No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCGG252.349093E-456.20044353
TCCCACG200.006265918753.8971663
GTCGTAT800.051.75840839
GTACTAG200.007692618351.172693
TCGTATG1950.049.60516440
GGTATCA14700.046.1917081
TGTCGTA900.045.93794638
CCGTCTT2500.040.39909747
CGTATGC2400.040.35579341
CACGAGA3000.039.81401421
GCCGTCT2550.039.57295646
TGCCGTC2550.039.51076545
ATGCCGT2550.039.4731744
GTTTGCG350.001356743439.3753838
GCGATGT1150.038.81175234
CGAGACA3350.038.71586223
CGAGCCC3000.038.66019415
ATGTCGT1100.037.54835537
GAGCGAT2850.037.2977932
CGGGTAT557.985411E-637.21650717
Produced by FastQC (version 0.11.5)