FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069464
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51590.48239117913272445No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40270.37654376397896516No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38310.3582168263728372No Hit
GTAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT35680.3336250682584921No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35460.33156796301698793No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32050.29968283177367355No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30260.2829454754905261No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGGTACCTAGAGCCTGAAGATT29980.28032734154679356No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATAA28340.2649925570192171No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28150.26321596612882714No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27320.2554550690813342No Hit
GTTTACTACTGTCAGTCTCGTTACGACTGGCCGACGTTCGGCCAAGGGAC27240.2547070308116963No Hit
GTGTTGACACAGTCTCCAGCCACCATGTCTTTGTCTCCAGGGGACACAGC27100.25339796383983004No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC27010.25255642078648743No Hit
GTTCCTTTACTTTCCACCTTGGTCCCTTGGCCGAACGTCGGCCAGTCGTA26660.24928375335682174No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25260.23619308363815894No Hit
GTCGTAACGAGACTGACAGTAGTAAACTGCAAAATCTTCAGGCTCTAGGTACCTGATGGTGAGAGTGAAGT25140.23507102623370213No Hit
CTCCTGCAGGGCCAGTCGGAGTATTGGCAACTACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGACT25070.234416492747769No Hit
CGTTACGACTGGCCGACGTTCGGCCAAGGGACCAAGGTGGAAAGTAAAGG24950.23329443534331218No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24930.2331074257759027No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23410.21889469865278305No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23310.2179596508157357No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22570.21104029682158537No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22360.20907669636378595No Hit
GACTTCACTCTCACCATCAGGTACCTAGAGCCTGAAGATTTTGCAGTTTACTACTGTCAGTCTCGTTACGACTGG21870.20449496196225397No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21660.20253136150445455No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21450.20056776104665514No Hit
GTAGTAAACTGCAAAATCTTCAGGCTCTAGGTACCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20780.19430294053843794No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20610.19271335921545746No Hit
GTCCCAGACCCACTGCCACTGAACCTGGTTGGGACGCCAGCGGCCCTGTT20490.19159130181100065No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20380.19056274919024857No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTACTTTCCACCTTGGTCC20050.18747709132799234No Hit
CTACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGACTCGTCGTCTATGATGCATCGAACAGGGCCG19530.18261484257534613No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17040.1593321514328673No Hit
GAACAGGGCCGCTGGCGTCCCAACCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGGTA16370.1530673309246501No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16340.1527868165735359No Hit
CTCCCAGACTCGTCGTCTATGATGCATCGAACAGGGCCGCTGGCGTCCCA16320.15259980700612644No Hit
CAGCCACCCTCTCCTGCAGGGCCAGTCGGAGTATTGGCAACTACTTAGCCTGGTACCAACAAAAACCTGGCCAGG16070.15026218741350808No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16070.15026218741350808No Hit
GAGTATTGGCAACTACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTC15960.149233634792756No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGTTGGGACGCCAGCGGCCCTGTTCGATGCATCATAGA15870.1483920917394134No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15820.14792456782088972No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15640.1462414817142045No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT15020.14044418512451098No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15010.14035068034080625No Hit
GTCTCCAGCCACCATGTCTTTGTCTCCAGGGGACACAGCCACCCTCTCCTGCAGGGCCAGTCGGAGTATTGGCAA14990.14016367077339678No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14710.13754553682966422No Hit
GATATAACCGGAGACATTGTGTTGACACAGTCTCCAGCCACCATGTCTTTGTCTCCAGGGGACACAGCCACCCT14350.13417936461629376No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14240.13315081199554168No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14240.13315081199554168No Hit
GGTACCTAGAGCCTGAAGATTTTGCAGTTTACTACTGTCAGTCTCGTTACGACTGGCCGACGTTCGGCCAAGGGA14130.1321222593747896No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14090.13174824023997067No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14080.13165473545626594No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13810.1291301062962381No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13380.12510940059693454No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13360.12492239102952507No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13300.12436136232729667No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13250.123893838408773No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13150.12295879057172565No Hit
GCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGACTCGTCGTCTATGATGCATCGAACAGGGCCGCTGGCGTC13150.12295879057172565No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13040.12193023795097357No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12950.12108869489763097No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12950.12108869489763097No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG12940.12099519011392623No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12910.12071467576281203No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12810.11977962792576469No Hit
CTGCTACTCTGGCTCCCAGATATAACCGGAGACATTGTGTTGACACAGTC12670.11847056095389841No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12340.11538490309164218No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA12330.11529139830793744No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12260.1146368648220043No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12240.11444985525459483No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12180.11388882655236643No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12060.11276676914790962No Hit
GGACCAAGGTGGAAAGTAAAGGAACTGTGGCTGCACCATCTGTCTTCATC12050.11267326436420488No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11750.10986812085306286No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11640.10883956823231075No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11580.10827853953008235No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11570.10818503474637761No Hit
GTGCAGCCACAGTTCCTTTACTTTCCACCTTGGTCCCTTGGCCGAACGTC11570.10818503474637761No Hit
ATGATGCATCGAACAGGGCCGCTGGCGTCCCAACCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCA11400.10659545342339713No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11360.10622143428857819No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11050.10332278599373143No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG11040.1032292812100267No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10810.10107867118481781No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGT350.00500115357.05382570
TCGATTG250.00775691651.06417569
GTGGTAT3900.047.1516041
GGTATCA15350.042.151451
TGGTATC4650.039.5298582
GTATCAA37150.036.5747531
GTCTTAC502.0092302E-434.0539361
GACAGGT2500.032.6917761
TATCAAC42150.032.141782
GTGAATA450.00410879631.41393356
ATCAACG43250.031.2353673
TCAACGC43500.031.2093894
TTAACGG1001.493936E-830.95420335
TTATGCG553.5324896E-430.9320684
CAACGCA44550.030.4752375
AACGCAG44900.030.2376826
ACGCAGA48000.028.2848327
CGCAGAG47900.028.130778
ATAATAC1104.186404E-827.8414633
CGAACTA1005.4044904E-727.36609629
Produced by FastQC (version 0.11.5)