FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1298752
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGGT100400.7730498201350219No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT84140.6478527078302863No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC68660.5286613610604642No Hit
GTACAAACTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACG64210.4943976987138422No Hit
GAGTTTGTACAGATTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT52600.4050041886364757No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51220.39437860345932096No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC50900.39191469965012565No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG48250.37151049623022714No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT47600.3665056916177992No Hit
ATCCAATACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC46310.35657307938698074No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC43260.33308899620558813No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG42250.3253122998078155No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG40830.3143787266545114No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT39300.30259818656679643No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG37540.28904671561622236No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA37430.2881997486818115No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT36620.2819629921647859No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35820.2758032326417976No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC35740.27518725668949884No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA34930.2689505001724733No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34090.26248275267333565No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTATTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC33360.2568619721086089No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33100.2548600502636377No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG32940.25362809835904004No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32870.2530891194007786No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32370.24923926969891097No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT31490.2424635342236239No Hit
CTTCCTATACTATGCATTGGGTGCGCCAGGCCCCCGGACAAAGGCTTGAG31310.24107758833095158No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG31150.23984563642635393No Hit
GTCAGGGACCCCGGAGGCCCGAATAGACCCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA30040.23129897008820777No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29460.22683314443404132No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29270.2253702015473316No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGGTCTATTCGGGCC28770.22152035184546398No Hit
GCATAGTATTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC28730.22121236386931456No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC27800.21405164342384073No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA27410.21104876065638398No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC27230.20966281476371162No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG26830.20658293500221753No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA26650.20519698910954515No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC25560.1968043167594737No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG25400.19557236485487606No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25070.19303146405164343No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA25040.19280047306953135No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24730.19041356625437342No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24550.1890276203617011No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA24250.1867177105405805No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA24220.18648671955846843No Hit
GTTTATTACTGCATGCAATCTGTACAAACTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA24150.18594774060020697No Hit
CTCCTGCATAGTATTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT24040.1851007736657961No Hit
TTATTACTGCATGCAATCTGTACAAACTCCGATCACCTTCGGCCAAGGGA23750.18286786083871284No Hit
GTAATATATAGCCGTGTCCTCAGATCTCAGGCTGCTCAGGTCCATGTAGG23740.1827908638446755No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC23710.18255987286256345No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAATCTGTACAAACT23600.18171290592815256No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23490.18086593899374168No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23230.1788640171487705No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22580.17385921253634257No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT22330.17193428768540875No Hit
ATTCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCAC22300.1717032967032967No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC22210.17101032375696051No Hit
GTATAGGAAGTGAGGGTGTATCCAGAAGCCTTGCAGGAAATTTTCACTGAGGCCCCAGGCTTCTTCACCTCAGCC21760.16754545902527965No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC21690.16700648006701818No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC21490.16546654018627113No Hit
ATTTGGGGTCTATTCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGT20670.15915278667520819No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20210.15561092494948997No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20150.15514894298526585No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGAGTTTGTACAGATTGCATGCAGTAATAAACCCCAACATCCTCAGCCT19790.15237705119992115No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19700.151684078253585No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19700.151684078253585No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19620.15106810230128617No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG19520.15029813236091263No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAATCTGTACAAACTCCGATCACCTTCGGCCAAGGGA19260.14829621051594147No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19090.14698726161730646No Hit
ATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGA19030.14652527965308235No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18630.14344539989158822No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18140.13967254718375796No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGGTCTATTCGGGCCTCCGGGGTCCCTGA17910.1379016163208988No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCAATACTATGC17890.13774762233282412No Hit
CTCCACAGCTCCTGATCTATTTGGGGTCTATTCGGGCCTCCGGGGTCCCT17770.1368236584043759No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17730.1365156704282265No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAATCTGTACAAACTCCGATCACCTTCGGCCAAGGGACA17560.13520672152959148No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA17440.13428275760114325No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17260.1328968117084709No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17230.13266582072635882No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT17190.13235783275020943No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC17160.13212684176809739No Hit
AGTCAGAGCCTCCTGCATAGTATTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG17110.13174185679791062No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17110.13174185679791062No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT17110.13174185679791062No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16960.1305869018873503No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16950.13050990489331296No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16920.1302789139112009No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16700.12858498004237914No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16660.12827699206622972No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT16590.12773801310796828No Hit
ATACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTC16580.1276610161139309No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16260.12519711230473562No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGGTCTATTCGGGCCTCCGGGGTCCCTGACAGGTT16220.12488912432858622No Hit
GTCTCCACAGCTCCTGATCTATTTGGGGTCTATTCGGGCCTCCGGGGTCC16150.12435014537032474No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16050.12358017542995121No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16040.12350317843591385No Hit
ATATATTACTGTGCGAGAGATGATTGTAGTGGTGCCAGCTGCTTTCTCGG16020.12334918444783916No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15900.12242522051939093No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15880.12227122653131622No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGGTCTATT15740.12119326861479328No Hit
GGCCGAAGGTGATCGGAGTTTGTACAGATTGCATGCAGTAATAAACCCCA15720.12103927462671857No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG15720.12103927462671857No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAAATTTCCTGCAAGGCTTCTGGATACACCCTCACTTC15560.11980732272212093No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15410.11865236781156063No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15370.11834437983541121No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15330.11803639185926182No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15190.11695843394273887No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA15120.1164194549844774No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT15030.11572648203814125No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14960.11518750307987977No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT14920.11487951510373035No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC14680.11303158724683389No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14580.11226161730646035No Hit
GCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCC14430.11110666239590006No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC14170.10910474055092889No Hit
GGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAATCTGTA14130.10879675257477947No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14050.10818077662248067No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13970.10756480067018184No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13940.10733380968806977No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13850.1066408367417336No Hit
AGTCTCCACAGCTCCTGATCTATTTGGGGTCTATTCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGAT13520.10409993593850095No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT13260.10209801409352978No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATAGTATTGGATACAACTATTTGG13240.10194402010545507No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13190.1015590351352683No Hit
GTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCA13160.10132804415315627No Hit
GATTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGAT13120.10102005617700685No Hit
CAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGGTCTATTCGGGC13110.1009430591829695No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)