FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1298752
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGGT92620.7131461587739615No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT85270.6565533681565072No Hit
GTACAAACTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACG59620.4590560784506973No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC59190.45574520770709115No Hit
GAGTTTGTACAGATTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT55500.4273333169073079No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC54340.418401665598975No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC52100.401154338934608No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT51360.3954565613758439No Hit
ATCCAATACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC43900.3380168038239787No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42900.3303171044202434No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42810.32962413147390723No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG40410.3111448529049426No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT39000.3002882767456758No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG38150.2937435322525009No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT37410.2880457546937368No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC37290.28712179076528854No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA36720.2827329621051594No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35800.27564923865372293No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT34530.26587062041097914No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34390.2647926624944562No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTATTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC33580.25855590597743067No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG31960.24608239294337947No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31820.24500443502685657No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA31740.24438845907455772No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT29950.2306059971418716No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29770.22922005124919922No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29170.22460023160695808No Hit
CTTCCTATACTATGCATTGGGTGCGCCAGGCCCCCGGACAAAGGCTTGAG28850.2221363277977628No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28610.22028839994086633No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28590.2201344059527916No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT28400.2186714630660819No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27530.2119727245848322No Hit
GTCAGGGACCCCGGAGGCCCGAATAGACCCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA27510.21181873059675752No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA27080.20850785985315132No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC26980.20773788991277783No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26760.20604395604395606No Hit
CTCCTGCATAGTATTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT25850.19903722958655692No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGGTCTATTCGGGCC25730.19811326565810872No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC25560.1968043167594737No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG24980.19233849110530724No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG24780.1907985512245602No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA24600.18941260533188786No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24510.18871963238555167No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC24220.18648671955846843No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24010.18486978268368404No Hit
GCATAGTATTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC23930.18425380673138522No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23580.18155891194007787No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC23460.1806349480116296No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA23050.17747807125609816No Hit
GTTTATTACTGCATGCAATCTGTACAAACTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA22900.17632311634553788No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAATCTGTACAAACT22350.17208828167348347No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA22330.17193428768540875No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA22230.17116431774503524No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA22140.17047134479869908No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT21890.16854641994776523No Hit
TTATTACTGCATGCAATCTGTACAAACTCCGATCACCTTCGGCCAAGGGA21840.1681614349775785No Hit
GTATAGGAAGTGAGGGTGTATCCAGAAGCCTTGCAGGAAATTTTCACTGAGGCCCCAGGCTTCTTCACCTCAGCC21170.16300263637707585No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC21130.16269464840092643No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21020.16184768146651554No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGAGTTTGTACAGATTGCATGCAGTAATAAACCCCAACATCCTCAGCCT20900.16092371753806733No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20680.15922978366924553No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20560.15830581974079733No Hit
ATTTGGGGTCTATTCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGT20530.15807482875868525No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC20230.1557649189375647No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC20210.15561092494948997No Hit
GTAATATATAGCCGTGTCCTCAGATCTCAGGCTGCTCAGGTCCATGTAGG20210.15561092494948997No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG19890.1531470211402947No Hit
ATTCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCAC19650.15129909328339822No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19450.14975915340265114No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAATCTGTACAAACTCCGATCACCTTCGGCCAAGGGACA19210.1479112255457547No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19130.14729524959345586No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAATCTGTACAAACTCCGATCACCTTCGGCCAAGGGA19110.14714125560538116No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18990.14621729167693293No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18760.14444636081407383No Hit
GGCCGAAGGTGATCGGAGTTTGTACAGATTGCATGCAGTAATAAACCCCA18430.14190546001084117No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18160.13982654117183263No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18150.1397495441777953No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17970.13836359828512296No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA17950.13820960429704823No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA17750.13666966441630118No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCAATACTATGC17440.13428275760114325No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17320.13335879367269501No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT17120.13181885379194797No Hit
ATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGA17110.13174185679791062No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17030.13112588084561178No Hit
CTCCACAGCTCCTGATCTATTTGGGGTCTATTCGGGCCTCCGGGGTCCCT16940.1304329078992756No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16620.1279690040900803No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16610.12789200709604295No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT16430.12650606120337063No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT16310.12558209727492237No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16170.12450413935839945No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT16140.12427314837628739No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15980.12304119647168973No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAAATTTCCTGCAAGGCTTCTGGATACACCCTCACTTC15760.12134726260286798No Hit
AGTCAGAGCCTCCTGCATAGTATTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG15710.1209622776326812No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15690.12080828364460651No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15460.1190373527817474No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15460.1190373527817474No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGGTCTATTCGGGCCTCCGGGGTCCCTGA15440.11888335879367269No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15430.11880636179963533No Hit
ATATATTACTGTGCGAGAGATGATTGTAGTGGTGCCAGCTGCTTTCTCGG15430.11880636179963533No Hit
GTCTCCACAGCTCCTGATCTATTTGGGGTCTATTCGGGCCTCCGGGGTCC15330.11803639185926182No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15240.11734341891292564No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15220.11718942492485093No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT15130.11649645197851476No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGGTCTATT15020.11564948504410388No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14970.11526450007391711No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG14810.11403254816931946No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGGTCTATTCGGGCCTCCGGGGTCCCTGACAGGTT14460.11133765337801212No Hit
ATACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTC14340.1104136894495639No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14340.1104136894495639No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14180.10918173754496624No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14080.10841176760459273No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14050.10818077662248067No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13990.10771879465825654No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13870.1067948307298083No Hit
GCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCC13750.10587086680136008No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13660.10517789385502391No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13630.10494690287291183No Hit
GGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGG13590.10463891489676243No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT13530.10417693293253831No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13510.1040229389444636No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13080.10071206820085744No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC13060.10055807421278273No Hit
GATTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGAT13020.10025008623663333No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCA252.4915402E-455.5356944
CGTGTAA200.00770565151.151410
CGGGTAT200.00771622751.13365617
TAGCGGT200.00772681351.115937
GGTATCA7950.049.2789961
TGGTATC1900.041.224452
GTATCAA21250.039.4373131
ACCCAGG7300.039.05872370
GCTTGTA350.001434254138.9334681
GTGGTAT2100.038.9334681
AATGGTA4100.036.42759770
TAGCGGC707.0851274E-735.93086264
TATCAAC23550.035.139532
ATGGCAA1953.45608E-1034.8142570
ATCAACG24250.034.4034163
TCAACGC24450.034.261274
CAACGCA24600.034.0562935
AACGCAG24850.033.5882646
ACGCAGA25450.032.6713457
CGCAGAG25850.032.3063358
Produced by FastQC (version 0.11.5)