FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1418499
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54180.381953036272849No Hit
ATATTACGGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGCTGGAAATCAGACGAACTGTGGCTGCACCAT54010.380754586362063No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGCTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49800.3510753268067161No Hit
CCGTAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTC46950.3309836665376571No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40000.2819882143025832No Hit
CCCTGGTTGTGTCCCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAGC39100.2756434794807751No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35680.2515334871579042No Hit
CTCCTAAAGTGCTCTTTTATTGGGCGTCTACCCGGGAGTCCGGAGTCCCT35410.24963006671136181No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34070.24018346153222525No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33760.2379980528713802No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32860.23165331804957212No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32640.2301023828709079No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32520.22925641822800014No Hit
CTTTAGGAGGCTGTCCGGGCTTCTGCTGGAACCAACTTAAGAAGTGCTTA31710.22354615688837284No Hit
GTTTATTACTGTCAGCAATATTACGGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGCTGGAAATCAGACGAA30890.2177653984951699No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGCTATCCACCTTCCACTGTACTTTGGCCT29710.20944674617324371No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28750.2026790290299817No Hit
GTCCCTGACCGATTCAGTGCCAGCGCGTCTGGGACAGATTTCACTCTCAC27650.19492435313666065No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACGCGCTGGCA27350.19280944152939128No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27070.1908355240292732No Hit
GTACAGTGGAAGGTGGATAGCGCCCTCCAATCGGGTAACTCCCAGGAGAG26980.19020105054709238No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26380.18597122733255364No Hit
GTCTCTGGTGCCTCCATGACAAACTTCTTCTGGATGTGGATTCGGCAGCC23660.16679602875997798No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGAGTTCACCGTCAGGAAGAGTTGATTCTTGGACGCGTCAATGGA23140.16313018197404439No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCAGCTTGGTCC23090.16277769670616618No Hit
CTTTTATTGGGCGTCTACCCGGGAGTCCGGAGTCCCTGACCGATTCAGTGCCAGCGCGTCTGGGACAGATTTCA22950.16179073795610713No Hit
CTTCTTAAGTTGGTTCCAGCAGAAGCCCGGACAGCCTCCTAAAGTGCTCTTTTATTGGGCGTCTACCCGGGAGTC22880.1612972585810776No Hit
ATTCAGTGCCAGCGCGTCTGGGACAGATTTCACTCTCACCATCAACAGCC22420.15805439411659788No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22350.1575609147415684No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22340.15749041768799274No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22160.1562214707236311No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22140.1560804766164798No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21880.15424755322351302No Hit
CCCCAACACTAATAAGCACTTCTTAAGTTGGTTCCAGCAGAAGCCCGGAC21640.15255562393769753No Hit
GATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTACGGTGCT21240.1497357417946717No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21180.14931275947321782No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21120.14888977715176394No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC20790.14656337438376765No Hit
CAGTAATAGACGGCCGTGTCCGCGGCGGTCACAGAGTTCACCGTCAGGAA20780.14649287733019198No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20030.14120559831201854No Hit
GCTTAAGCCTTCGGAAACCCTGTCTCTCACTTGCACTGTCTCTGGTGCCT19790.13951366902620305No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGCTATCCA19480.13732826036535803No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18980.13380340768657573No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG18950.1335919165258488No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAGCGCCCTCCAATCG18950.1335919165258488No Hit
GTGCAGCCACAGTTCGTCTGATTTCCAGCTTGGTCCCTTGGCCGAACGTC18950.1335919165258488No Hit
CTCCCTGGTTGTGTCCCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAGCCAGAGTATTTTTTATAGTTCCTC18650.13147700491857944No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18430.12992606973991522No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18410.12978507563276392No Hit
GCCAAGGGACCAAGCTGGAAATCAGACGAACTGTGGCTGCACCATCTGTC18200.12830463750767537No Hit
GTGCCAGCGCGTCTGGGACAGATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTA18190.12823414045409973No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT17950.12654221116828424No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17880.1260487317932547No Hit
GTCAGGGACTCCGGACTCCCGGGTAGACGCCCAATAAAAGAGCACTTTAGGAGGCTGTCCGGGCTTCTGCTGGAA17870.12597823473967903No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17770.1252732642039226No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT17670.12456829366816614No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17510.12344034081095581No Hit
CTATTACTGTGCTCGCTGGCCCACACAGGTGGGCCTGGGAAAGATTGTGTGGGGCCAGGGACTACTGGTCACCGT17400.1226648732216237No Hit
CACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTACGGTGCTCCGTGGACGTT17320.12210089679301854No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17190.12118443509653513No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT17130.12076145277508127No Hit
ACCCGGGAGTCCGGAGTCCCTGACCGATTCAGTGCCAGCGCGTCTGGGACAGATTTCACTCTCACCATCAACAGC17120.12069095572150562No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16870.11892852938211447No Hit
GTGCAAGTCCAGCCAGAGTATTTTTTATAGTTCCTCCATCAGTTCGAACC16820.11857604411423625No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16750.11808256473920672No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16690.11765958241775284No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16470.11610864723908863No Hit
CACTTGCACTGTCTCTGGTGCCTCCATGACAAACTTCTTCTGGATGTGGA16460.116038150185513No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16240.11448721500684879No Hit
CTAATAAGCACTTCTTAAGTTGGTTCCAGCAGAAGCCCGGACAGCCTCCTAAAGTGCTCTTTTATTGGGCGTCTA16170.11399373563181928No Hit
GAGTGAAATCTGTCCCAGACGCGCTGGCACTGAATCGGTCAGGGACTCCG16120.11364125036394104No Hit
GTCTATTACTGTGCTCGCTGGCCCACACAGGTGGGCCTGGGAAAGATTGT16020.11293627982818459No Hit
GCCTCCTAAAGTGCTCTTTTATTGGGCGTCTACCCGGGAGTCCGGAGTCCCTGACCGATTCAGTGCCAGCGCGT15930.11230180634600377No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG15920.11223130929242814No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15900.11209031518527683No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15590.10990490652443181No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15560.10969341536370487No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15520.1094114271494023No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAGCGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15510.10934093009582664No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15510.10934093009582664No Hit
TCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACGCGCTGGCACTGAATCGGTCAGGGACT15440.10884745072079713No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15280.1077194978635868No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15280.1077194978635868No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15270.10764900081001115No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15130.1066620420599521No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACGCGCTGGCA15080.10630955679207386No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15050.10609806563134692No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15020.10588657447061998No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14910.1051111068812879No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC14810.10440613634553145No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT14600.10292569822044288No Hit
AATAAGCACTTCTTAAGTTGGTTCCAGCAGAAGCCCGGACAGCCTCCTAAAGTGCTCTTTTATTGGGCGTCTACC14430.10172724830965689No Hit
GCCACAGTTCGTCTGATTTCCAGCTTGGTCCCTTGGCCGAACGTCCACGG14260.10052879839887092No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14240.10038780429171962No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC200.00617126754.105963
TACGTAT400.00876663449.50669570
GGTATCA13900.045.0405461
TGGTATC3450.039.4425472
CGTATGC2850.038.77113741
AATCTCG2950.038.36377736
TCGTATG2900.038.0495640
ATGCCGT2950.037.599544
GCTAATC3100.037.5217333
GTATCAA34100.037.4177361
GTGGTAT3550.037.379791
ATCTCGT3000.036.62808637
CGAGACT3450.036.49593723
ATCTCCG3600.035.9040910
GACAGGT3150.035.6454471
TACACAT3950.035.3110665
ACGAGAC3600.034.9691122
CACGAGA3650.034.4888621
TCTCCGA3650.034.48156411
CCGTCTT3350.034.3049147
Produced by FastQC (version 0.11.5)